L-Lactate Assay Kit (Colorimetric) ab65331 from Abcam

Product Specs
ItemL-Lactate Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65331
MethodFuncS
Quantity100 Test
Sample TypeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate
Assay Time0h 30m
SensitivityEqual to 0.02 mM
Detection Range0.02 - 20 mM
Research AreaMetabolism

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Focused Ultrasound‐Induced Mechanical Ablation Affects the Carbohydrate Metabolism of Residual/Peri‐Focally Localized Glioblastoma CellsInternational Journal of CancerAugust 15, 2026Frieda Bayler, Jonna Holler, Jacqueline Clüver, Levi Johanning, Dana Hellmold, Nils Oliver Schröder, Jessica Nojszewski, Hajrullah Ahmeti, Carolin Kubelt‐Kwamin, Michael Synowitz, et al.lactate assay was performed according to the manufacturer's instructions using the L‐Lactate assay kit (ab65331, Abcam, Rozenburg, The Netherlands). Specifically, after homogenizing 0.3 × 106 mFUS (un)treated GSCs

(2) Phosphorylation-Facilitated CKB Lactylation At K11 By GCN5 Enhances Creatine Kinase Activity and Mitigates Neuronal Damage After Cerebral Ischemia-ReperfusionAdv Sci (Weinh)July 17, 2026Chao Duan, Ruolin Zhang, Shihui Ding, Ying Feng, Tao Shen, Ziwei Wang, Wendai Bao, Ke Shui, Jun Qin, Jun Chen, et al.min at 4°C, lactate levels in the supernatant were quantified using a Lactate Colorimetric Assay Kit (ab65331, Abcam) according to the manufacturer's protocol. 4.4 Proteomics Protein digestion and peptide preparation:

(3) Targeting TKT-associated immunometabolic remodeling attenuates experimental lupus nephritis and NET-related inflammationFront Cell Dev BiolJuly 1, 2026Litong Zhu, Taoyan Lin, Lai Yee Cheong, An Ying Christy Chui, Tak Mao Chan, Desmond Y. H. Yapto assess proteinuria. Lactate measurement Mouse Serum Lactate Assay Kit was purchased from Abcam (ab65331, United Kingdom). Serum lactate was measured according to the procedures specified by the manufacturer. Elisa Mouse

(4) Both L-lactyl and D-lactyl enantiomers modify histones in mouse testisMolecular & Cellular ProteomicsJune 12, 2026Julie Manessier, Hassan Hijazi, Lisa Vizzini, Sabine Brugière, Marie Courçon, Christophe Masselon, Alberto de la Iglesia, Julie Cocquet, Delphine Pfliegerthree-month-old mice were used to estimate the abundance of both enantiomers, L- and D-lactate. Kit ab65331 for dosing L-lactate and kit ab83429 for dosing D-lactate were purchased from Abcam. Each testis was

(5) FOSL2 drives transcriptional activation of super‑enhancer-regulated DYNC1H1 to promote hypoxia‑induced oral squamous cell carcinoma progressionMolecular Medicine ReportsMay 25, 2026Linyu Jin, Jing Zhang, Wenjing Wang, Nan Zhang, Yang Liu, Alin Tian, Weiwei Yin, Yongle Qiu(cat. no. ab136955; Abcam). Lactate production was measured using an L-Lactate Assay Kit (cat. no. ab65331; Abcam). Statistical analysis Quantitative data are presented as mean ± standard deviation from at

(6) M6A-modified lncRNA HULC promotes osteosarcoma progression by stabilizing GLUT1 mRNA through interaction with TAF15Cell Oncol (Dordr)May 25, 2026Bo Liu, Yang Yue, Chaoqun Cen, Yong Liproduction, and ATP levels were quantified using the Glucose Assay Kit (ab65333, Abcam), L-Lactate Assay Kit (ab65331, Abcam), and ATP Assay Kit (ab83355, Abcam), respectively, following the manufacturers’ instructions. Western blot (WB

(7) Oncogenic PIK3CA mutations shape an immunoregulatory microenvironment in mosaic overgrowth disordersPNAS NexusMay 13, 2026Ilaria Galasso, Charles Bayard, Camille Blériot, Sophia Ladraa, Wan Ting Kong, Rubina Cassaca, Clément Hoguin, Sanela Protic, Jérôme Megret, Nicolas Goudin, et al.Diagnostics). Lactate concentration To calculate lactate concentration, we used L-Lactate Assay Kit (Colorimetric) AB65331 from Abcam following manufacture’s protocol. Lactate was calculated for AdipoCreER mice plasma and primary

(8) PARP1-BCAT2 axis upregulates ABCG1 via histone lactylation to drive acquired PARP inhibitor resistance in prostate cancerJournal of Experimental & Clinical Cancer ResearchMay 11, 2026Wangli Mei, Hang Zhou, Weiyi Li, Yongqiang Liu, Chaozhi Tang, Mengyu Wei, Zhen Zhou, Bowen Ye, Hanwen Niu, Weitian Wang, et al.listed in Supplementary Table 2. Lactate assay L-lactate was quantified using an L-Lactate Assay Kit (ab65331; Abcam, UK) per the manufacturer’s protocol. Standard curves were generated on 96-well plates using

(9) PKM2 mediates the glycolytic response to matrix stiffness in lung fibroblastsFront Cell Dev BiolMay 1, 2026Yuan Wang, Ruihan Hu, Xu Zhang, George Cai, Qiang Ding, Pulin Checontrol group. Lactate production assay Lactate levels were measured using the L-lactate assay kit (Abcam, ab65331) according to the manufacturer’s instructions and as described previously (Che et al., 2021). Briefly,

(10) Hypertension Drives Protein Lactylation and Vascular Dysfunction in Skeletal MuscleHypertensionApril 22, 2026Milene T. Fontes, Paul Townsend, Landon Butler, Juliana M. Parente, Fênix A. Araujo, Tiago J. Costa, Gisele F. Bomfim, Laena Pernomian, Wenbin Tan, Brandon N. VanderVeen, et al.were collected, and lactate concentration was measured using a quantitative lactate assay kit (Abcam; #ab65331 for muscle; #ab65330 for plasma). Muscle samples were deproteinized using the Deproteinizing Sample Preparation Kit-TCA

(11) Hypertension Drives Protein Lactylation and Vascular Dysfunction in Skeletal MuscleHypertensionApril 22, 2026Milene T. Fontes, Paul Townsend, Landon Butler, Juliana M. Parente, Fênix A. Araujo, Tiago J. Costa, Gisele F. Bomfim, Laena Pernomian, Wenbin Tan, Brandon N. VanderVeen, et al.were collected, and lactate concentration was measured using a quantitative lactate assay kit (Abcam; #ab65331 for muscle; #ab65330 for plasma). Muscle samples were deproteinized using the Deproteinizing Sample Preparation Kit-TCA

(12) ASO-based PKM splice-switching therapy increases anti-CTLA-4 antibody efficacy in pancreatic ductal adenocarcinomaCell DiscovApril 21, 2026Lijie Han, Lina Gan, Balázs Schäfer, Dillon M. Voss, Mathias Danielsen, Ondrej Kostov, Jia Liu, Ting Wang, Marvin H. Caruthers, Hao Chen, et al.kit (ab83432; Abcam), and lactic acid production was measured with a L-Lactate Assay Kit (ab65330 and ab65331; Abcam) in accordance with the manufacturer’s instructions. Cells were either transfected with 50 nM

(13) Glycaemic variability underlies myocyte dysfunction and myocardial injury risk in diabetesNature CommunicationsApril 18, 2026Yuanzhao Cao, Meredith A. Redd, Jennifer E. Outhwaite, Dalia Mizikovsky, Woo Jun Shim, Chen Fang, Courtney Vedelago, Clarissa Tan, Zhixuan Wu, Dara Daygon, et al.exposed to control, cG, vG conditions were measured using a colorimetric L-Lactate Assay Kit (Abcam, Cat.#ab65331) according to the manufacturer’s instructions. In brief, supernatant samples were prepared (1:300 dilution)

(14) Icariin improves metabolic response to exercise by promoting TFEB-dependent mitochondrial clearance and metabolic reprogramming in C57BL/6 mice and C2C12 myotubesFront NutrApril 13, 2026Zhengyuan Liu, He Hu, Liang Zhenglactate concentration in the cell culture medium was measured using a commercial Lactate Assay Kit (AB65331, Abcam) according to the manufacturer's instructions. 2.5.2 LDH activity assay Intracellular LDH activity

(15) Protein phosphatase 2A orchestrates hematopoietic fate determination via modulation of lactate metabolismHaematologicaApril 1, 2026Can Liu, Yao Meng, Heng Chen, Siqi Bi, Ye Tian, Zhihua Yin, Guanhua Li, Wutao Chen, Li Wu, You Wang, et al.software. The levels of L-lactate in the cell lysate were quantified using the L-Lactate Assay kit (ab65331, Abcam). Quantification the enzymatic activities of histone deacetylases Overexpressed HDAC1-Flag

(16) MEF2A activates the ZDHHC20/NF-κB pathway to inhibit doxorubicin sensitivity and promote the malignant progression of acute myeloid leukemiaBiology DirectMarch 26, 2026Xi Ming, Wanying Liu, Jiaying Wu, Sijia Yan, Shuai Su, Yu Wang, Rubing Zheng, Yi Xiaoconsumption, lactate production, and ATP levels using Glucose Assay Kit (ab136955, Abcam), Lactate Assay Kit (ab65331; Abcam), and ATP detection kit (S0026, Beyotime), respectively. Quantitative real-time polymerase chain reaction

(17) BDNF facilitates ectopic endometrial stromal cell growth, invasion, migration and glycolysis in endometriosis via upregulating GLUT1CytojournalMarch 21, 2026Yapeng Han, Yang Yu, Yaguang Han, Wei Guan, Jieming Li, Boyi Feng, Chang Sun, Hong Ying Kuangratio were evaluated in ectopic ESCs using the glucose assay kit (ab65333, Abcam), L-Lactate assay kit (ab65331, Abcam), and ADP/ATP ratio assay kit (ab65313, Abcam), respectively. Co-immunoprecipitation (Co-IP)

(18) BDNF facilitates ectopic endometrial stromal cell growth, invasion, migration and glycolysis in endometriosis via upregulating GLUT1CytojournalMarch 21, 2026Yapeng Han, Yang Yu, Yaguang Han, Wei Guan, Jieming Li, Boyi Feng, Chang Sun, Hong Ying Kuangratio were evaluated in ectopic ESCs using the glucose assay kit (ab65333, Abcam), L-Lactate assay kit (ab65331, Abcam), and ADP/ATP ratio assay kit (ab65313, Abcam), respectively. Co-immunoprecipitation (Co-IP)

(19) MALAT1 Regulates Human Macrophage Metabolism by Interacting with HADHBiScienceMarch 20, 2026Yuxiang Liu, Yukiteru Nakayama, Junichi Sugita, Tsukasa Oshima, Kunihito Kani, Atsushi Kobayashi, Naoto Setoguchi, Yoshiko Iwai, Ichiro Manabe, Katsuhito Fujiu37612 NEBNext Ultra II RNA Library Prep Kit,NEB,Cat# E7770L L-lactate Assay Kit (Colorimetric),Abcam,Cat# ab65331 Deproteinizing Sample Preparation Kit-TCA,Abcam,Cat# ab204708 MycoFluor Mycoplasma Detection Kit,Thermo Fisher

(20) ATAD2 drives immunotherapy resistance by promoting lactic acid-mediated CD8+ T cell dysfunction in lung adenocarcinomaFrontiers in ImmunologyMarch 19, 2026Wanfeng Gao, Jialei Xu, Yue Li, Jingchang Zhang, Chenghao Ma, Junfeng Chen, Jiajing Chencells, as well as in their corresponding tumor tissues, were measured with a Lactate Assay Kit (Abcam, ab65331) according to the manufacturer’s protocol. In vivo animal studies C57BL/6 mice were purchased from

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L-Lactate Assay Kit (Colorimetric) from Abcam

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L-Lactate Assay Kit (Colorimetric) from Abcam

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