L-Lactate Assay Kit (Colorimetric) ab65331 from Abcam

Product Specs
ItemL-Lactate Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65331
MethodFuncS
Quantity100 Test
Sample TypeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate
Assay Time0h 30m
SensitivityEqual to 0.02 mM
Detection Range0.02 - 20 mM
Research AreaMetabolism

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(1) Overexpression of endoplasmic reticulum acetyl-CoA transporter AT-1 alters neuronal metabolism, growth, and synaptic activityiScienceNovember 20, 2026Eric R. McGregor, Cassandra J. McGill, Nicholas L. Arp, Josef P. Clark, Dominique A. Baldwin, Yusi Cui, Alexander Nassar, Di Kuang, Gonzalo Fernandez-Fuente, Yun Hwa Choi, et al.levels Intracellular and extracellular lactate were measured using a colorimetric L-Lactate assay kit (ab65331; Abcam). Media was collected and assessed following the manufacturer’s instructions. Intracellular levels

(2) Non-Canonical HIF2 Accumulation in Breast Cancer CellsInt J Mol SciOctober 20, 2026Karolina Kozal, Aleksandra Opitz, Monika Jarosiewicz, Michał Juszczak, Anna Krześlakconducting assays, samples were diluted, and the Glucose Assay Kit ab65333 and L-Lactate Assay Kit ab65331 (Abcam, Cambridge, UK) were performed according to the manufacturer’s instructions. Glucose consumption

(3) Lactylation-Mediated HER2 Stabilization Promotes Trastuzumab Resistance in Gastric CancerAdv Sci (Weinh)October 11, 2026Meng Qian, Tongguan Tian, Zhenxiang Wang, Huihui Sun, Ming Zong, Ying Chen, Jie Xiong, Ye Chen, Zhiqian Hu, Tianming Li, et al.supernatants were collected. Then, lactate levels were measured using an L‐Lactate Assay Kit (Abcam ab65331). 4.4 Enzyme Linked Immunosorbent Assay (ELISA) The concentration of HER2 was measured using a commercial

(4) Context-specific hypermetabolism in humans with mitochondrial defectsCell Press BlueOctober 7, 2026Evan D Shaulson, Alexander J Sercel, Shannon Rausser, Samantha Leonard, Kathryn Whyte, Faris Zuraikat, Heather Seid, Qiuhan Huang, Alex Junker, Darshana Kapri, et al.lactate was quantified in MiSBIE samples using a colorimetric enzymatic L-Lactate Assay Kit (Abcam, ab65331). Plasma samples were thawed on ice and 16 μL aliquots were diluted with 184 μL of Lactate Assay Buffer

(5) Indole-3-Propionic Acid Disrupts Glycolytic Adaptation and Promotes Oxidative Stress-Associated Injury in Oral Squamous Cell CarcinomaInt J Mol SciOctober 5, 2026Negar Taghavi Pourianazarquantified in conditioned medium using colorimetric assay kits (Abcam, Cat. No. ab65333, Abcam, Cat. No. ab65331, Abcam, Cat. No. ab83355). Intracellular adenosine triphosphate content was assessed using a commercial

(6) AARS2-SIRT5-Driven DLD K445 Lactylation Promotes Cuproptosis Resistance in Glioblastoma Stem CellsAdv Sci (Weinh)September 29, 2026Mingtian Ding, Haibiao Xu, Cen Liu, Yangyin Ding, Shuai Wang, Jun Yin, Tao Fu, Xiao Jiang, Yuning Chen, Hui Luo, et al.Levels Intracellular lactate levels were measured using a lactate colorimetric/fluorometric assay kit (ab65331, Abcam) according to the manufacturer's instructions. Briefly, cells were collected and lysed to obtain

(7) PLOD1 Catalytic Activity Stabilizes ENO1 by Limiting FBXW7-dependent Degradation to Promote Glycolysis and TMZ Resistance in GlioblastomaAdv Sci (Weinh)September 27, 2026Fen Xue, Dingkong Liang, Xin Chen, Fei Fan, Xiayun Heand ATP levels were quantified using a Glucose Assay Kit (Abcam, ab65333), Lactate Assay Kit (Abcam, ab65331), and ATP Assay Kit (Colorimetric/Fluorometric; Abcam, ab83355), respectively, according to the manufacturers'

(8) ADCK1 Regulates Mitochondrial Bioenergetics in Hepatocellular Carcinoma In VitroInt J Mol SciSeptember 8, 2026Noel Jacquet, Yunfeng Zhaothe accompanying software. 4.4. Cellular Lactate Assay The Abcam L-Lactate Colorimetric Assay Kit (ab65331) (Novi, MI, USA) was used to measure the intracellular lactate levels. Cells were cultured overnight

(9) The inhibition of lactate/Gpr81 signalling driven by moderate-intensity continuous training protects against osteoarthritisExperimental & Molecular MedicineSeptember 3, 2026Peng Sun, Yilan Guo, Aochuan Xue, Jingting Ni, Yichen Ren, Yan Sun, Xingyu Qian, Xiaoliu Li, Yu You, Ying Zhao, et al.muscle-derived lactate is minimal. Blood lactate levels were measured using an L-lactate assay kit (Abcam, ab65331) in strict accordance with the manufacturer’s instructions. Cell culture Primary articular chondrocytes

(10) Exosomal circ-ZNF652 Mediates TGF-β1-Induced Epithelial-Mesenchymal Transition and Glycolytic Reprogramming to Promote Hepatocellular Carcinoma ProgressionJournal of Microbiology and BiotechnologySeptember 2, 2026Xianli Niu, Xiaomin Zhang, Guangxia Liang, Nina Wei, Haibo Peng, Xiangyang LiDetection Kit (Beyotime, China); Glucose Uptake Assay Kit (ab136955, Abcam, UK); Lactate Assay Kit (ab65331, Abcam, UK); S-P immunohistochemistry kit (Beijing Zhongshan Biotechnology Co., Ltd., China); MTT reagent,

(11) Ubiquitination of parkin influences its tumor suppressive function in cervical cancerCellular and Molecular Life SciencesAugust 26, 2026Yuanyuan Mai, Hui Hua, Shicong Liang, Guiqin Ye, Sangni Qian, Zixuan Li, Wenjie Zeng, Xiaoyan Chen, Qinghui Zheng, Huafeng Shou, et al.levels in HeLa cells expressing Parkin WT or Parkin mutant were measured with lactate and pyruvate kits (ab65331, Abcam), respectively, and their relative levels were calculated according to the instructions for user.

(12) Glycolytic enolase 1-induced H4K12 lactylation activates quiescent breast cancer stem-like cells in triple-negative breast cancerSignal Transduct Target TherAugust 21, 2026Shuai Liu, Yunfeng Ma, Yujie Zeng, Dekang Lv, Zhijie Hou, Junxiu Huang, Yaqi Gong, Ruijun Feng, Zihan Wang, Ran Sui, et al.lactate concentration was measured using cell lysates with a Lactate Colorimetric Assay Kit (Abcam, Cat #ab65331) according to the manufacturer’s instructions. Intracellular glucose was measured using cell lysates

(13) Oxygen-dependent osteocyte transcriptomic adaptations occur without modulation of endothelial gene expression via osteocyte paracrine signalingJBMR PlusAugust 19, 2026Léa Gellée, Frédéric Martins, Alexandre Brochard, Katharina Jähn-Rickert, Delphine B Maurel(Varioskan Lux, Thermoscientific). Lactate levels were quantified using an L-lactate assay kit (Abcam, ab65331), samples were diluted 1:30 and incubated with the reaction mix for 30 min at room temperature prior

(14) Focused Ultrasound‐Induced Mechanical Ablation Affects the Carbohydrate Metabolism of Residual/Peri‐Focally Localized Glioblastoma CellsInternational Journal of CancerAugust 15, 2026Frieda Bayler, Jonna Holler, Jacqueline Clüver, Levi Johanning, Dana Hellmold, Nils Oliver Schröder, Jessica Nojszewski, Hajrullah Ahmeti, Carolin Kubelt‐Kwamin, Michael Synowitz, et al.lactate assay was performed according to the manufacturer's instructions using the L‐Lactate assay kit (ab65331, Abcam, Rozenburg, The Netherlands). Specifically, after homogenizing 0.3 × 106 mFUS (un)treated GSCs

(15) Phosphorylation-Facilitated CKB Lactylation At K11 By GCN5 Enhances Creatine Kinase Activity and Mitigates Neuronal Damage After Cerebral Ischemia-ReperfusionAdv Sci (Weinh)July 17, 2026Chao Duan, Ruolin Zhang, Shihui Ding, Ying Feng, Tao Shen, Ziwei Wang, Wendai Bao, Ke Shui, Jun Qin, Jun Chen, et al.min at 4°C, lactate levels in the supernatant were quantified using a Lactate Colorimetric Assay Kit (ab65331, Abcam) according to the manufacturer's protocol. 4.4 Proteomics Protein digestion and peptide preparation:

(16) Radiation induces an EGFR-dependent lactate-epigenetic program underlying synergy with EGFR inhibitionCell Oncol (Dordr)July 4, 2026Hongxia Cheng, Lu Meng, Shilan Luo, Chenhui Zhao, Xiesong Luo, Jingdan Pang, Qingyu Jiang, Yanghai Xiong, Peiwen Zhu, Kun Guo, et al.glucose uptake. Lactate levels were measured using the L-Lactate Assay Kit (Colorimetric) (Abcam, #ab65331) according to the manufacturer’s instructions. For intracellular lactate measurement, cells were collected

(17) Targeting TKT-associated immunometabolic remodeling attenuates experimental lupus nephritis and NET-related inflammationFront Cell Dev BiolJuly 1, 2026Litong Zhu, Taoyan Lin, Lai Yee Cheong, An Ying Christy Chui, Tak Mao Chan, Desmond Y. H. Yapto assess proteinuria. Lactate measurement Mouse Serum Lactate Assay Kit was purchased from Abcam (ab65331, United Kingdom). Serum lactate was measured according to the procedures specified by the manufacturer. Elisa Mouse

(18) Both L-lactyl and D-lactyl enantiomers modify histones in mouse testisMolecular & Cellular ProteomicsJune 12, 2026Julie Manessier, Hassan Hijazi, Lisa Vizzini, Sabine Brugière, Marie Courçon, Christophe Masselon, Alberto de la Iglesia, Julie Cocquet, Delphine Pfliegerthree-month-old mice were used to estimate the abundance of both enantiomers, L- and D-lactate. Kit ab65331 for dosing L-lactate and kit ab83429 for dosing D-lactate were purchased from Abcam. Each testis was

(19) TRIM47 drives metabolic reprogramming and tumor progression in Nasopharyngeal Carcinoma via K48-linked ubiquitination and degradation of SDHBCellular and Molecular Life SciencesJune 6, 2026Jieqing Yu, Le Ding, Yong Yang, Tao Xie, Junbo Peng, Qing Luo, Xuan Huang, Yong Li, Jing Yelactate production were measured using a glucose assay kit (ab65333, Abcam, USA) and L‐lactate assay kit (ab65331, Abcam, USA) according to the manufacturers’ instructions, respectively. Statistical analysis Data

(20) M6A-modified lncRNA HULC promotes osteosarcoma progression by stabilizing GLUT1 mRNA through interaction with TAF15Cell Oncol (Dordr)May 25, 2026Bo Liu, Yang Yue, Chaoqun Cen, Yong Liproduction, and ATP levels were quantified using the Glucose Assay Kit (ab65333, Abcam), L-Lactate Assay Kit (ab65331, Abcam), and ATP Assay Kit (ab83355, Abcam), respectively, following the manufacturers’ instructions. Western blot (WB

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L-Lactate Assay Kit (Colorimetric) from Abcam

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L-Lactate Assay Kit (Colorimetric) from Abcam

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