Nitric Oxide Assay Kit (Colorimetric) ab65328 from Abcam

Product Specs
ItemNitric Oxide Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65328
MethodFuncS
Quantity2 x 96 Tests
Sample TypeUrine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
Assay Time1h 30m
SensitivityEqual to 1 nmol/well
Detection Range1 - 10 nmol/well

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) From Insight to Impact: Spotlight on the Potential Implications of the Neuronal Nitric Oxide Synthase Isoenzyme (nNOS) in Experimental Chronic ToxoplasmosisDiseasesJuly 3, 2026Marwa Omartested mice using the colourimetric nitric oxide assay kit (Abcam Inc., Waltham, MA, USA, Cat. No. ab65328) following the directions of the manufacturer. A microtiter plate was filled with 85 μL of each serum

(2) Bilobalide increases SKCa current via 5-HT2BR-PLC–PKC pathway, causing hyperpolarization and enhanced EET production in human coronary artery endothelial cellsBMC Complementary Medicine and TherapiesMay 28, 2026Katesirin Ruamyod, Patcharaporn Taokum, Neennara Wongsatan, Sompol Tapechum, Wattana B. Watanapaby measuring its products, nitrate and nitrite, in the supernatant using a commercial NO assay kit (ab65328, Abcam). Briefly, nitrate was first converted to nitrite with nitrate reductase, and then nitrite reacted

(3) Neuroprotective effects of rutin, sodium selenite, and rutin-conjugated selenium nanoparticles in a social isolation modelFront PharmacolApril 15, 2026Khaled M. Alam-ElDein, Abanoub A. S. Shaker, Manal F. El-Khadragy, Ibrahim K. M. Alabbadi, Ahmed E. Abdel Moneim, Alzahraa A. Elhemiely, Nievin Ahmed Mahran, Triveena M. Ramsis, Shaimaa H. El-Fayoumi, Mohamed A. Ali, et al.2017), MDA (Cat. No. ab118970, Abcam, Cambridge, UK) (Misra and Fridovich, 1972), and NO (Cat. No. ab65328, Abcam, Cambridge, UK) (Green et al., 1982), with absorbance readings recorded at 540 nm. The antioxidant

(4) Novel Pyrazole-3-Cyano-2-Pyridinone Hybrids as Multitarget Anti-Inflammatory Agents: Synthesis, Computational Modeling, and Biological EvaluationDrug Des Devel TherApril 1, 2026Bahgat R M Hussein, Ibrahim M Salem, Hossameldin A Aziz, Omar Alshazly, Heba A Hofny, Aliaa M Mohassab, Zuhier A Awan, Tarek S Ibrahim, Elsayed M Mahmoud, Stefan Bräse, et al.12.98. Biological Evaluation Inhibitory Activity of NO Over-Production NO colorimetric assay kit (ab65328, Abcam Co., Waltham, MA, USA) was used to analyze the content of NO in brain tissue homogenate, following

(5) Novel Pyrazole-3-Cyano-2-Pyridinone Hybrids as Multitarget Anti-Inflammatory Agents: Synthesis, Computational Modeling, and Biological EvaluationDrug Des Devel TherApril 1, 2026Bahgat R M Hussein, Ibrahim M Salem, Hossameldin A Aziz, Omar Alshazly, Heba A Hofny, Aliaa M Mohassab, Zuhier A Awan, Tarek S Ibrahim, Elsayed M Mahmoud, Stefan Bräse, et al.12.98. Biological Evaluation Inhibitory Activity of NO Over-Production NO colorimetric assay kit (ab65328, Abcam Co., Waltham, MA, USA) was used to analyze the content of NO in brain tissue homogenate, following

(6) Varying Perfusate Hemoglobin During Normothermic Ex Situ Heart Perfusion: Elevated Hemoglobin Exacerbates Functional Decline Through HemolysisTransplant DirectMarch 20, 2026Mitchell J. Wagner, Guilherme Mainardi Aguiar da Silva, Parham Hassanzadeh, Sanaz Hatami, Xiuhua Wang, Jayan Nagendran, Darren H. Freedof biochemical parameters in the perfusate were accomplished via commercial kits: Total NOx (Abcam, ab65328), Total iron binding capacity (TIBC) and serum iron (Abcam ab239715), and cardiac troponin-I (Life Diagnostics,

(7) Varying Perfusate Hemoglobin During Normothermic Ex Situ Heart Perfusion: Elevated Hemoglobin Exacerbates Functional Decline Through HemolysisTransplant DirectMarch 20, 2026Mitchell J. Wagner, Guilherme Mainardi Aguiar da Silva, Parham Hassanzadeh, Sanaz Hatami, Xiuhua Wang, Jayan Nagendran, Darren H. Freedof biochemical parameters in the perfusate were accomplished via commercial kits: Total NOx (Abcam, ab65328), Total iron binding capacity (TIBC) and serum iron (Abcam ab239715), and cardiac troponin-I (Life Diagnostics,

(8) Fibrinogen exacerbates α-synuclein aggregation and mitochondrial dysfunction via alpha5beta3 integrin in Parkinson’s diseaseJournal of Advanced ResearchMarch 1, 2026Zifeng Huang, Jialing Zheng, Feilan Yuan, Hui Zhong, Ruoyang Yu, Yuqi Luo, Muwei Zhang, Shuhan Chen, Bibiao Shen, Zhenchao Xie, et al.manufacturer’s instructions, NO levels in SH-SY5Y cells were determined by an NO assay kit (Abcam, ab65328, MA). Mitochondrial complex I kits (Solarbio, Cat# BC0515) were used to detect mitochondrial complex

(9) Potentiating anti-inflammatory and antioxidant effects in vitro: the combined action of zofenoprilat and nebivololPharmacological ReportsFebruary 3, 2026Eleonora Maceroni, Annamaria Cimini, Michele d’Angelo, Marta Sofia Scenna, Suada Meto, Simone Baldini, Paolo Fabrizzi, Giovambattista Desideri, Vanessa Castelliwas determined by assessing the concentration of NO metabolite in fresh samples using Abcam assay (#ab65328) supplemented by a 10 kDa spin column (#ab93349, Abcam). Briefly, the samples and standards were added

(10) Intraoral applicability of plasma-activated water: antibacterial activity, cytotoxicity, and effects on tooth structureBMC Oral HealthDecember 5, 2025Ha-yun Noh, Sung Un Kang, Hee-Kyung Kim, Sun Park, Chul-Ho Kim, Joo-Hee Kim, Seung-Yoon Limnitric oxide, and hydrogen peroxide (H₂O₂) were quantified using a commercial Nitric Oxide Assay Kit (ab65328, Abcam, Cambridge, UK) and a Peroxide (H₂O₂) Assay Kit (MAK311, Sigma-Aldrich, USA) according to the

(11) Cellular fibronectin exacerbates α-synuclein aggregation via integrin alpha4beta1 mediated PARP1 and SCD elevationNeurotherapeuticsOctober 1, 2025Zifeng Huang, Hui Zhong, Yingqiong Lu, Ruoyang Yu, Muwei Zhang, Jialing Zheng, Bin Xiao, Zhidong Zhou, Yinghua Yu, Chao Deng, et al.fatty acid and triglyceride assay NO levels in SH-SY5Y cells were measured via an NO assay kit (Abcam ab65328 MA) according to the manufacturer's instructions. Mitochondrial complex I kits (Solarbio, China) were

(12) Targeting the Epigenetic Remodeler GCN5 Prevents Vascular Oxidative Stress and Endothelial Dysfunction in Obesity: Insights in Patients with Cardiometabolic DiseaseHigh Blood Pressure & Cardiovascular PreventionSeptember 8, 2025Sarah Costantino, Shafeeq Mohammed, Alessandro Mengozzi, Emiliano Duranti, Valentina Delfine, Martin A. Geiger, Nazha Hamdani, Stefano Taddei, Stefano Masi, Agostino Virdis, et al.oxide levels The levels of nitric oxide (NO) were detected by using a colorimetric activity assay (ab65328, Abcam) in vascular homogenates and cell lysates. Mitochondrial swelling assay Forty µg of isolated

(13) Klotho protein alleviates heart ischemia/reperfusion injury and oxidative stress through regulation of the NOS/MMP pathwayScientific reportsSeptember 2, 2025Olejnik A, Krzywonos-Zawadzka A, Sambor I, Mysiak A, Bil-Lula I.heart tissue served as a measure of NO production3,16. A commercially available Nitric Oxide Assay Kit (ab65328, Abcam, Cambridge, MA, USA) according to the manufacturer’s instructions for measurement of (NOx)– level

(14) Macula densa nitric oxide synthase 1β restoration by kidney alkalization enhances renal graft outcomesAmerican journal of physiology. Renal physiologySeptember 1, 2025Parris CL, Liu C, Rani A, Tran MH, Li M, Esquivel C, Oropeza A, Wang L.homogenized tissue according to the manufacturer’s instructions (Abcam, Nitric Oxide Assay Kit, colorimetric, ab65328) (34, 35). Immunoblotting The levels of the interested proteins were measured with immunoblotting

(15) Insects as Sentinels of Oxidative Stress Induced by Environmental Contaminants: Biomarkers and Analytical ApproachesToxicsAugust 20, 2025Marcello Messi, Roberta Giorgione, Maria Luisa Astolfitissues,Al2O3 NPs (0.03 mg/g body weight),Single dose injection,Increased(*),"Nitric oxide assay kit (ab65328, Abcam Co., Berlin, Germany).",-,[13] Locusta migratoria,Testicular tissues,Single dose injection,"Nitric oxide assay kit

(16) Antihypertensive Effects of Lotus Seed (Nelumbo nucifera Gaertn.) Extract via eNOS Upregulation and Oxidative Stress Reduction in L-NAME-Induced Hypertensive RatsPharmaceuticals (Basel)August 4, 2025Anjaree Inchan, Tippaporn Bualeong, Worasak Kaewkong, Nitra Nuengchamnong, Phapada Apaikawee, Pakaporn Sa-Nguanpong, Wiriyaporn Sumsakul, Natthawut Charoenphon, Usana Chatturong, Watcharakorn Deetud, et al.used as indirect markers of NO presence. Plasma NO levels were measured using the colorimetric NO Kit (ab65328; Abcam Cambridge, MA, USA) following the manufacturer’s protocol. The method involves a two-step process:

(17) Ginsenoside Re increases human coronary artery endothelial SKCa current and nitric oxide release via glucocorticoid receptor-PI3K-Akt/PKB pathwayJournal of Ginseng ResearchApril 29, 2025Kitinat Rodthongdee, Wattana B. Watanapa, Katesirin Ruamyod, Namoiy Semprasert, Pimchanok Nambundit, Suwattanee Kooptiwut, Luecha Boontaveekulmeasured in triplicate using an indirect quantitative colorimetric method based on the Griess reaction (AB65328, Abcam, Cambridge, UK). The optical densities (OD) were read at 540 nm with a microplate reader (Bio-Rad

(18) The Interplay of Nitric Oxide and Nitrosative Modifications in Maize: Implications for Aphid Herbivory and Drought StressInternational Journal of Molecular SciencesOctober 20, 2024Hubert Sytykiewicz, Paweł Czerniewicz, Magdalena Ruszczyńska, Katarzyna Kmiećin the maize samples were measured with the application of the Nitric Oxide Assay Kit (catalogue no. ab65328; Abcam, Cambridge, UK) and the Peroxynitrite Assay Kit (catalogue no. ab233469; Abcam, Cambridge, UK),

(19) Non-thermal atmospheric pressure plasma induces selective cancer cell apoptosis by modulating redox homeostasisCell Communication and SignalingSeptember 26, 2024Ju Hyun Yun, Yoon Hee Yang, Chang Hak Han, Sung Un Kang, Chul-Ho Kimwith excitation set at 540 nm. NO3˗ level was determined using a Colorimetric Nitric Oxide Assay Kit (ab65328, Abcam) and detected using an EPOCH microplate reader (BioTek). Cell viability Cell viability assays

(20) Toloese Generates Nitric Oxide through Natural Radiation of Far Infrared Rays, Reducing Serum Glucose, Cholesterol, and TriglyceridesHealthcare (Basel)June 20, 2024Min-Ho Yeo, Young-Hyeon Lee, Mi-Jin Ryu, Yong-Hak Choi, Hye-Sook Kim, Kyung-Soo Changthe manufacturer’s instructions. Serum NO was measured using a nitric oxide colorimetric assay kit (ab65328, Abcam, Cambridge, MA, USA). Serum collected via SST tubes was mixed with nitrate reductase to convert

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Nitric Oxide Assay Kit (Colorimetric) from Abcam

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Nitric Oxide Assay Kit (Colorimetric) from Abcam

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