Urea Assay Kit ab83362 from Abcam

Product Specs
ItemUrea Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab83362
MethodFuncS
Quantity100 test
Assay Time1h 0m
Sensitivity0.5 nmol/well
ApplicationsCellular Assay
TypeQuantitative
Detection Range0.5 - 5 nmol/well
Detection TargetUrea
Sample TypeUrine, Serum, Plasma, Tissue Extracts, Cell Lysate, Cell culture media

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Rat liver extracellular matrix and perfusion bioreactor culture promote human amnion epithelial cell differentiation towards hepatocyte-like cellsJ Tissue EngDecember 22, 2023Sara Campinoti, Bruna Almeida, Negin Goudarzi, Stefan Bencina, Fabio Grundland Freile, Claire McQuitty, Dipa Natarajan, I Jane Cox, Adrien Le Guennec, Vamakshi Khati, et al.(E88-129, Bethyl Laboratories, FORTIS Life Sciences, Waltham, Massachusetts, USA); Urea Assay Kit (ab83362 Abcam, Cambridge, UK), following manufacturer’s instructions. The concentration (ng/mL) for each sample

(2) Antidiabetic Effect of Fermented Mesembryanthemum crystallinum L. in db/db Mice Involves Regulation of PI3K-Akt PathwayCurr Issues Mol BiolAugust 3, 2023Hye-Lin Kim, Yunu Jung, Hyo In Kim, Nak-Yun Sung, Min-Jee Kim, In-Jun Han, Geon Kim, Eun Yeong Nho, Sang-Yun Park, Yohan Han, et al.(Cheongju, Chungcheongbuk-do, Republic of Korea). Creatinine (ab65340) and blood urea nitrogen (BUN) (ab83362) were measured using kits purchased from Abcam Inc. (Cambridge, MA, USA). 2.6. Histological Analysis

(3) The effects of a comparatively higher dose of 1000 mg/kg/d of oral L- or D-arginine on the L-arginine metabolic pathways in male Sprague-Dawley ratsPLoS OneAugust 1, 2023Dain (Raina) Kim, Sarah Martin, Kaushik DesaiFinland). Urea and hydroxyproline assays Urea levels were measured with a colorimetric Urea Assay Kit (Cat. # ab83362, Abcam Inc., Toronto, ON, Canada), according to kit instructions. The optical density was read at 570

(4) In vitro construction of liver organoids with biomimetic lobule structure by a multicellular 3D bioprinting strategyCell ProliferationMay 17, 2023Honglei Jian, Xin Li, Qianqian Dong, Shaonan Tian, Shuo Baisecretions in the supernatant were determined using the ELISA kit (ab179887, Abcam) and urea assay kit (ab83362, Abcam), respectively. ALB and urea contents were assayed by absorbance at 450 nm and 462 nm on a microplate

(5) Parthenolide Phytosomes Attenuated Gentamicin-Induced Nephrotoxicity in Rats via Activation of Sirt-1, Nrf2, OH-1, and NQO1 AxisMoleculesMarch 17, 2023Rawan S. Albalawi, Lenah S. Binmahfouz, Rawan H. Hareeri, Rasheed A. Shaik, Amina M. Baghermeasure serum levels of creatinine (Cat. No. E4370-100, BioVision, Milpitas, CA, USA), urea (Cat. No. ab83362, Abcam, Cambridge, UK), and cystatin C (Cat. No. CSB-E08385r, CUSABIO, Fannin, TX, USA) were used. 4.7.

(6) Modulatory Role of Autophagy in Metformin Therapeutic Activity toward Doxorubicin-Induced NephrotoxicityToxicsMarch 16, 2023Antar SA, Abd-Elsalam M, Abdo W, Abdeen A, Abdo M, Fericean L, Raslan NA, Ibrahim SF, Sharif AF, Elalfy A, et al.creatinine and urea were assessed in accordance with their manufacturer’s instructions (ab204537 and ab83362, respectively; Abcam, Cambridge, UK). 4.4. Assessment of NGAL Assay of NGAL was performed using the

(7) Myrrh Essential Oil Mitigates Renal Ischemia/Reperfusion-Induced InjuryCurrent Issues in Molecular BiologyFebruary 1, 2023Nancy S. Youniscreatinine (Cr, Cat. No. ab700460), uric acid (Cat. No. ab65344), blood urea nitrogen (BUN, Cat. No. ab83362), and lactate dehydrogenase (LDH, Cat. No. ab102526), using the specified colorimetric or ELISA kit

(8) Targeting endogenous kidney regeneration using anti-IL11 therapy in acute and chronic models of kidney diseaseNature communicationsDecember 5, 2022Widjaja AA, Viswanathan S, Shekeran SG, Adami E, Lim WW, Chothani S, Tan J, Goh JWT, Chen HM, Lim SY, et al.Biosciences). The levels of blood urea nitrogen (BUN) in mouse serum were measured using Urea Assay Kit (ab83362, Abcam). Albumin and creatinine levels were measured using Mouse Albumin ELISA kit (ab108792, Abcam)

(9) Salmonella pathogenicity island 1 knockdown confers protection against myocardial fibrosis and inflammation in uremic cardiomyopathy via down-regulation of S100 Calcium Binding Protein A8/A9 transcriptionRenal failureDecember 1, 2022Cai X, Hong L, Liu Y, Huang X, Lai H, Shao L.employed to measure blood creatinine (ab65340, Abcam, Cambridge, UK) and blood urea nitrogen (BUN; ab83362, Abcam), respectively. Inflammatory cytokines, interleukin-6 [(IL-6), ab234570], tumor necrosis factor-alpha

(10) Inhibition of glutaminolysis restores mitochondrial function in senescent stem cellsCell reportsNovember 29, 2022Choudhury D, Rong N, Ikhapoh I, Rajabian N, Tseropoulos G, Wu Y, Mehrotra P, Thiyagarajan R, Shahini A, Seldeen KL, et al.K455-100 Pyruvate Assay Kit,Abcam,Cat# ab65342 Ammonia Assay Kit,Abcam,Cat# ab83360 Urea Assay Kit,Abcam,Cat# ab83362 DCF ROS/RNS Assay Kit,Abcam,Cat# ab238535 Senescence Detection Kit,Abcam,Cat# ab65351 Experimental models

(11) Immunological and Pathological Peculiarity of Severe Acute Respiratory Syndrome Coronavirus 2 Beta VariantMicrobiology spectrumOctober 26, 2022Lee S, Yoon GY, Lee SJ, Kwon YC, Moon HW, Kim YJ, Kim H, Lee W, Jeong GU, Kim C, et al.using an AST activity assay kit (no. ab105135), ALT assay kit (no. ab105134), and urea assay kit (no. ab83362) (Abcam), respectively, according to the manufacturer’s instructions. For Western blot analysis, cellular

(12) Indirect 3D Bioprinting of a Robust Trilobular Hepatic Construct with Decellularized Liver Matrix HydrogelBioengineering (Basel)October 22, 2022Vamakshi Khati, Johannes Artturi Turkki, Harisha Ramachandraiah, Falguni Pati, Giulia Gaudenzi, Aman Russomwere quantified using the Human Albumin ELISA kit (ab179887, Abcam, Cambridge, UK) and Urea Assay Kit (ab83362, Abcam), according to the manufacturer’s protocol. The number of viable cells (only HepG2) at a particular

(13) A comprehensive transcriptomic comparison of hepatocyte model systems improves selection of models for experimental useCommunications BiologyOctober 14, 2022Arif Ibrahim Ardisasmita, Imre F. Schene, Indi P. Joore, Gautam Kok, Delilah Hendriks, Benedetta Artegiani, Michal Mokry, Edward E. S. Nieuwenhuis, Sabine A. Fuchssamples, respectively. Functional assays Urea secretion was measured using a urea assay kit (Abcam, ab83362) according to the manufacturer’s instructions. Briefly, 1 mM ammonium chloride was added to the culture

(14) Helicobacter pylori shows tropism to gastric differentiated pit cells dependent on urea chemotaxisNature communicationsOctober 5, 2022Aguilar C, Pauzuolis M, Pompaiah M, Vafadarnejad E, Arampatzi P, Fischer M, Narres D, Neyazi M, Kayisoglu Ö, Sell T, et al.measurement The urea concentration in the conditioned-organoid media was measured using a Urea Assay kit (ab83362, Abcam) and following the manufacturer’s instructions. Samples were collected after 24 h, centrifuged

(15) Stable nebulization and mucoâ€trapping properties of regdanvimab/ IN â€006 support its development as a potent, doseâ€saving inhaled therapy for COVID â€19Bioengineering & Translational MedicineAugust 30, 2022Morgan D. McSweeney, Ian Stewart, Zach Richardson, Hyunah Kang, Yoona Park, Cheolmin Kim, Karthik Tiruthani, Whitney Wolf, Alison Schaefer, Priya Kumar, et al.BALF collection. The concentrations of urea in BALF and serum were measured using a commercial kit (ab83362; Abcam). The kit allows quantification of urea in a variety of biological samples such as serum, BALF,

(16) Steviol glycosides as an alternative osmotic agent for peritoneal dialysis fluidFrontiers in PharmacologyAugust 16, 2022Valeria Kopytina et al.vein puncture 2 days before the surgery and at the end point, analyzing serum samples for urea levels (Ab83362; Abcam). PD fluid exposure and surgery Catheter implantation was performed as previously described

(17) Renal Ischemia/Reperfusion Mitigation via Geraniol: The Role of Nrf-2/HO-1/NQO-1 and TLR2,4/MYD88/NFκB PathwayAntioxidants (Basel)August 13, 2022Maged E. Mohamed, Mohammad A. Elmorsy, Nancy S. Youniscreatinine (Cr, Cat. No; ab700460), uric acid (Cat. No; ab65344), blood urea nitrogen (BUN, Cat. No; ab83362), lactate dehydrogenase (LDH, Cat. No; ab102526) and kidney injury molecule-1 (Kim-1, Cat. No; ab119597),

(18) 2-Methoxyestradiol TPGS Micelles Attenuate Cyclosporine A-Induced Nephrotoxicity in Rats through Inhibition of TGF-β1 and p-ERK1/2 AxisAntioxidants (Basel)July 30, 2022Mohammed W. Al-Rabia et al.C were determined using commercial kits (Cat. No. E4370-100, BioVision, Milpitas, CA, USA; Cat. No. ab83362, Abcam, Cambridge, UK, and Cat. No. CSB-E08385r, CUSABIO, Fannin, TX, USA, respectively). The BCA Protein Assay Kit

(19) Embelin and levodopa combination therapy for improved Parkinson’s disease treatmentTranslational NeuroscienceJune 29, 2022Vagdevi Hangarakatte Ramachandra, Senthilkumar Sivanesan, Anand Koppal, Shanmugam Anandakumar, Matthew D. Howell, Ethirajan Sukumar, Rajagopalan Vijayaraghavanthe following sources: rotenone (TCI Chemicals Pvt. Ltd., Chennai, India); the Abcam Urea Assay Kit ab83362 (Boston, MA, USA); thiobarbituric acid (TCI Chemicals Pvt. Ltd., Tamil Nadu, India); olive oil, Thuruthel

(20) Gut Microbiota Mediates the Protective Effects of Traditional Chinese Medicine Formula Qiong-Yu-Gao against Cisplatin-Induced Acute Kidney InjuryMicrobiology spectrumJune 29, 2022Zou YT, Zhou J, Zhu JH, Wu CY, Shen H, Zhang W, Zhou SS, Xu JD, Mao Q, Zhang YQ, et al.USA). Renal function determination. BUN in serum samples was measured using a commercial urea assay kit (ab83362, Abcam) in strict accordance to the manufacturer’s standard protocol. For the evaluation of urine output

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Urea Assay Kit from Abcam

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Urea Assay Kit from Abcam

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