D-Lactate Assay Kit (Colorimetric) ab83429 from Abcam

Product Specs
ItemD-Lactate Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab83429
MethodFuncS
Sample TypeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
Assay Time0h 40m
SensitivityMore than 0.01 mM
Detection Range0.01 - 10 mM
Quantity100 Test

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Gut microbiota dysbiosis exacerbates acute pancreatitis via Escherichia coli-driven neutrophil heterogeneity and NETosisGut MicrobesDecember 31, 2026Yaoyu Zou, Nianshuang Li, Xueyang Li, Maobin Kuang, Xin Xu, Langyi Guan, Xin Li, Pan Zheng, Leyan Li, Jianhua Wan, et al.(E-EL-M3063, Elabscience, China), Lipopolysaccharide(LPS) (CSB-E13066m, Cusabio, China), and D-lactate (AB83429, Abcam, UK), were quantified using commercial ELIZA kits according to manufacturers' instructions. Additionally,

(2) Exosome-like Nanovesicles from Hordeum vulgare L. Fermented with Lactiplantibacillus plantarum BMSE-HMP251 Ameliorate LPS-Induced Inflammation in HT-29 and RAW 264.7 CellsMoleculesFebruary 15, 2026Duna Yu, Jeong-Eun Lee, Jin Hong Kim, Jung Soo Kim, Si Jun Park, Ki-Young Kim, Hana Jung, Moochang KookSeoul, Republic of Korea). D-lactate concentrations were measured using a D-Lactate Assay Kit (cat. no. ab83429; Abcam, Cambridge, UK) according to the manufacturer’s instructions. Briefly, 50 µL of the filtered

(3) Superior thermotolerance in young versus adult rats undergoing heat stroke is associated with age-related differences in intestinal barrier integrity and heat shock protein responsesFrontiers in Cell and Developmental BiologyFebruary 13, 2026Yuanhao Cai, Lei Lei, Jikuai Chen, Xi Zhao, Juelin Chen, Jiawei Zhou, Yankun Pei, Yawei Wang, Yitong Gong, Jianyao You, et al.intestinal fatty acid-binding protein (I-FABP) were quantified using a D-Lactate colorimetric assay kit (ab83429, Abcam, Cambridge, United Kingdom) and an intestinal fatty acid-binding protein (IFABP) ELISA kit (KE00263,

(4) The potential of human milk oligosaccharides in ameliorating traumatic brain injury-induced cognitive impairment in miceFuture Sci OAFebruary 2, 2026Dongliang He, Renli He, Wei Duan, Guilan Li, Qin Kangmanufacturer’s instructions. The serum D-lactic acid was detected using the Abcam D-Lactate Assay Kit (ab83429) according to the manufacturer’s instructions. 4.6. Immunofluorescence staining Mice were anesthetized

(5) Glyoxalase 2 Drives D-Lactate Oncometabolite Signaling to Promote Prostate Cancer Aggressiveness via FAK/Src ActivationAntioxidants (Basel)January 28, 2026Dominga Manfredelli, Camilla Torcoli, Veronica Ceccarelli, Tatiana Armeni, Guido Bellezza, Vincenzo N. Talesa, Angelo Sidoni, Cinzia AntognelliD-Lactate Detection D-lactate levels were determined with the D-lactate Assay Kit (Colorimetric) (cat. ab83429, Abcam, Cambridge, MA, USA) [39,40]. 2.10. Migration and Invasion Assays Migration was assessed using

(6) High indigestible dietary protein impairs growth and health status of nursery pigs with Salmonella Typhimurium more than with enterotoxigenic Escherichia coli F4 challengeJournal of Animal ScienceJanuary 8, 2026Taiwo J Erinle, Marllon J K de Oliveira, John K Htoo, S Maria Mendoza, Jenny-Lee Thomassin, Daniel A ColumbusItem No: 706002, Cayman Chemical, Ann Arbor MI), malondialdehyde (MDA, ab118970; Abcam), d-Lactate (ab83429; Abcam), urea nitrogen (PUN, ab83362; Abcam), alkaline phosphatase (ALP, DALP-250; Gentaur, Bioassay Systems

(7) Restoring macrophage iron homeostasis by natural carrier-free nanoplatform for the therapy of rheumatoid arthritis syndromesMaterials Today BioDecember 1, 2025Na Jia, Jia Cui, Lei Wang, Zifeng Huang, Xingru Tao, Qianqian Dong, Jingwen Wanglactate in the serum of mice in each group were investigated using ELISA and lactate assay (Abcam, ab83429, USA) kits. 2.5 Micro-CT imaging All mice were euthanized on the 28th day after AIA induction. After

(8) Polyphyllin II inhibits thyroid cancer cell growth by simultaneously inhibiting glycolysis and oxidative phosphorylationOpen Med (Wars)October 27, 2025Jianwei Sun, Ding Ding, Qian Xiang, Mengyang Zheng, Mingming Daiproduction Prepare the reaction mix according to the instructions of the lactate uptake assay kit (Abcam, UK, cat: ab83429). Collect 2 × 106 cells in a centrifuge tube, wash once with PBS, and add 200 µL of Assay Buffer XII/Lactate

(9) Shenfu injection promotes self-renewal of intestinal stem cells in sepsis-induced intestinal injury via inducing ATF4 expressionFrontiers in PharmacologyJuly 31, 2025Yunxin Deng, Lingyun Lu, Jinrong Li, Liya Gao, Yihui Wang, Nataša Petrović, Fengyong Yang, You Li, Mei Mengdetecting absorbance at 450 nm following the manufacturer’s introductions (D-Lactate Assay Kit, Abcam, ab83429). 2.2 Shenfu injection Shenfu injection (SFI, batch number: 221101AK01) was obtained from Huarun Sanjiu

(10) Determining lactate concentrations in Korean indigenous calves and evaluating its role as a predictor for acidemia in calf diarrheaBMC Veterinary ResearchAugust 20, 2024Youngwoo Jung, Ji-Yeong Ku, Byoungsoo Kim, Youngjun Kim, Kwang-Man Park, Jonghun Baek, DoHyeon Yu, Jinho ParkD-lactate concentration was measured using a commercial colorimetric assay kit (D-Lactate Assay kit, ab83429, Abcam, Cambridge, MA, USA). Frozen serum was thawed at room temperature (about 23 °C) for one hour

(11) Butyrate increases methylglyoxal production through regulation of the JAK2/Stat3/Nrf2/Glo1 pathway in castration‑resistant prostate cancer cellsOncology reportsMay 1, 2024Hsia YJ, Lin ZM, Zhang T, Chou TC.reader (BioTek Instruments, Inc.). The intracellular D-Lactate was measured by D-Lactate assay kit (ab83429, Abcam). Briefly, the cells were lysed and collected the supernatants after centrifugation (12,000 ×

(12) Organoids transplantation attenuates intestinal ischemia/reperfusion injury in mice through L-Malic acid-mediated M2 macrophage polarizationNature communicationsOctober 25, 2023Zhang FL, Hu Z, Wang YF, Zhang WJ, Zhou BW, Sun QS, Lin ZB, Liu KX.before detection. In accordance with the manufacturer’s protocol, the colorimetric D-lactate assay kit (ab83429, Abcam) was used to measure D-lactate concentration in the plasma by researchers who were blinded to

(13) Exosomal circEZH2_005, an intestinal injury biomarker, alleviates intestinal ischemia/reperfusion injury by mediating Gprc5a signalingNature communicationsSeptember 6, 2023Zhang W, Zhou B, Yang X, Zhao J, Hu J, Ding Y, Zhan S, Yang Y, Chen J, Zhang F, et al.binding protein-2 (IFABP) protein levels (DFBP20; R&D Systems, Minneapolis, USA), human D-lactate (ab83429; Abcam), murine TNF-α (EK282/4-96; MultiSciences, Hangzhou, China), and murine IL-β (KGEMC004; KeyGEN

(14) Acid tolerance of lactate-utilizing bacteria of the order Bacteroidales contributes to prevention of ruminal acidosis in goats adapted to a high-concentrate dietAnimal NutritionSeptember 1, 2023Zhongyan Lu, Lingmeng Kong, Shenhao Ren, Jörg R. Aschenbach, Hong ShenD-lactate and ammonia nitrogen were determined with L-Lactate, D-Lactate, and Ammonia Assay Kits (ab65331, ab83429, and ab83360, Abcam Trading Company Ltd, Shanghai, China), respectively, and a microplate reader (Infinite M200

(15) Microbiota-derived tryptophan metabolites indole-3-lactic acid is associated with intestinal ischemia/reperfusion injury via positive regulation of YAP and Nrf2Journal of translational medicineApril 18, 2023Zhang FL, Chen XW, Wang YF, Hu Z, Zhang WJ, Zhou BW, Ci PF, Liu KX.assay kit Patients’ serum D-lactate levels were determined using D-Lactate Assay Kit (Colorimetric) (ab83429, Abcam, USA) as instructed by the manufacturer. Lactate dehydrogenase (LDH) levels LDH levels in culture

(16) High‑risk pathological subtype associated FAM83A‑AS1 promotes malignancy and glycolysis of lung adenocarcinoma via miR‑202‑3p/HK2 axisOncology ReportsMarch 22, 2023Xinkui Xiong, Lei Zhang, Bao Zang, Dafu Xu, Chen Chen, Gaochao Dong, Wenjie Xia, Yanhu Wuconcentrations. D-Lactate assay Intracellular lactate levels were measured using a D-Lactate Assay kit (cat. no. ab83429; Abcam) according to the manufacturer's instructions. Statistical analysis The statistical analysis

(17) S100A9 promotes glycolytic activity in HER2-positive breast cancer to induce immunosuppression in the tumour microenvironmentHeliyonFebruary 1, 2023Jia-qi Yuan, Shou-man Wang, Lei Guoabsorbance at 450 nm following the manufacturer’s introductions (Lactate Assay Kit, BioVision/Abcam, ab83429). 2.10 Detection of the extracellular acidification rate Seahorse XFe 96 Extracellular Flux Analyzer

(18) MicroRNA-155-5p inhibition alleviates irritable bowel syndrome by increasing claudin-1 and ZO-1 expressionAnnals of Translational MedicineJanuary 31, 2023Jian-Guo Guo, Yue-Feng Rao, Jun Jiang, Xin Li, Sheng-Mei Zhumurine abdominal aorta. Serum D-lactate (D‑LA) and diamine oxidase (DAO) were assessed using D-LA (ab83429, Abcam) and DAO (ab241004, Abcam) ELISA kits in accordance with the manufacturer’s instructions. Murine

(19) Fructose-1,6-Bisphosphatase 2 Inhibits Oral Squamous Cell Carcinoma Tumorigenesis and Glucose Metabolism via Downregulation of c-MycOxidative Medicine and Cellular LongevityMay 6, 2022Liang Wang, Jinbing Wang, Yi Shen, Zhiwei Zheng, Jian SunATP The D-lactate levels were measured in HSC-3 and CAL-27 cells using a D-lactate assay kit (cat.no. ab83429, Abcam) according to the manufacturer's procedure. The cellular ATP content was detected in HSC-3 and

(20) Functional attractors in microbial community assemblyCell SystemsJanuary 1, 2022Sylvie Estrela, Jean C.C. Vila, Nanxi Lu, Djordje Bajić, Maria Rebolleda-Gómez, Chang-Yu Chang, Joshua E. Goldford, Alicia Sanchez-Gorostiaga, Álvaro Sánchezkit,Millipore,Cat. #GAGO20 Acetate assay kit,Abcam,Cat. #ab204719 D-Lactate assay kit,Abcam,Cat. #ab83429 Succinate assay kit,Abcam,Cat. #ab204718 DNeasy 96 Blood & Tissue kit,QIAGEN,Cat. #69582 Quan-iTPicoGreen

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D-Lactate Assay Kit (Colorimetric) from Abcam

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D-Lactate Assay Kit (Colorimetric) from Abcam

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