Phosphate Assay Kit (Colorimetric) ab65622 from Abcam

Product Specs
ItemPhosphate Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65622
Quantity500 Test
Detection Range0.001 - 1 mM
MethodFuncS
Sample TypeSerum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
SensitivityMore than 1 µM
Assay Time2h 0m

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Website: http://www.abcam.com

(1) Targeted profiling of the serum proteome associates increased FGF-23 levels with postoperative delirium in cardiac surgical patientsNPJ DementApril 27, 2026Christopher Simon, Occam Kelly Graves, Va’Shayna E. Williams, Isabella Turco, Ariel Mueller, Miriam Trigo, Talia Collechi, Malek Mitchell, Oluwaseun Akeju, Tina B. McKaylevels were measured using a colorimetric phosphate assay kit according to the manufacturer’s protocol (ab65622; Abcam, Cambridge, MA, USA). Briefly, serum samples were diluted 200- to 400-fold, incubated with malachite

(2) Vitamin D Deficiency Activates Gdnf-Ret-pErk1/2 Signal and Induces Kidney Malformations in MiceInternational Journal of Molecular SciencesMarch 27, 2026Minghui Yu, Ningli Ye, Haixin Ju, Qianfan Miao, Chunyan Wang, Rufeng Dai, Jing Chen, Yihui Zhai, Lei Sun, Xiaohui Wu, et al.were measured using a Calcium Assay kit (ab102505, Abcam, Shanghai, China) or Phosphate Assay kit (ab65622, Abcam, Shanghai, China). 4.3. Phenotypic Analysis Gross phenotypic characteristics, including the

(3) Oncogenic GPRIN1 sustains proliferation and mitochondrial homeostasis via dual‑layer CDK1-PI3K/Akt signalling in gallbladder cancerCell Death & DiseaseMarch 21, 2026Chang Xu, Zijun Gong, Xiaojian Ni, Lingxi Nan, Wentao Sun, Houbao Liu, Xuanming Luo, Min Liphosphate assay Intracellular free phosphate levels were measured using a Phosphate Assay Kit (Abcam, Cat# ab65622) according to the manufacturer’s protocol. Absorbance was read at 650 nm. Cycloheximide (CHX) chase

(4) Restoring immune homeostasis in atherosclerotic plaques via inorganic violet phosphorus nano-immunotherapyCell Reports MedicineJanuary 20, 2026Wanqin Zeng, Yingjun Fan, Chaohua Wang, Shuping Yang, Junchao Xu, Caijin Wang, Hucheng Chang, Yaoyao Luo, Shengbin Liu, Zhongshan Hethe concentration of phosphate ions released into the supernatant using a Phosphate Assay Kit (Abcam, ab65622), following the manufacturer’s instructions. Briefly, a standard curve was generated using known concentrations

(5) Accelerated osteocytic citrate production in chronic kidney disease is associated with protection of the kidneybioRxivOctober 13, 2025Jie Ren Gerald Gerald Har, Maggie Yun-Hsuan Hsu, Keith L. Saum, Akshdeep Singh, Ruchir Sriram, Dylan P. Kuennen, Emily K. Zhu, Subramaniam Pennathur, Maciek R. Antoniewicz, Lauren E. SurfaceMicroVue™ Mouse/Rat FGF-23 ELISA (Intact),QuidelOrtho,60-6800 Calcium Assay Kit (Colorimetric),Abcam,bb102505 Phosphate Assay Kit (Colorimetric),Abcam,ab65622 Deposited data,Deposited data,Deposited data RNASeq of tibia cortices,GEO,Will include once repository

(6) Redox-dependent liver gluconeogenesis impacts different intensity exercise in miceNature MetabolismSeptember 18, 2025Takahiro Horiuchi, Keizo Kaneko, Shinichiro Hosaka, Kenji Uno, Seitaro Tomiyama, Kei Takahashi, Maya Yamato, Akira Endo, Hiroto Sugawara, Yohei Kawana, et al.FUJIFILM Wako), ketone bodies (ab272541, Abcam), NAD(H) (N509, Dojindo), ATP (ab83355, Abcam) and Pi (ab65622, Abcam) according to the relevant instructions for each. Tolerance tests of gluconeogenic substrates Mice

(7) Phosphate salt selection affects mortality and vascular calcification in the adenine-induced chronic kidney disease mouse modelAmerican Journal of Physiology-Heart and Circulatory PhysiologySeptember 1, 2025Valentina Dargam, Sophie K. Ashbrook, Perony da Silva Nogueira, Amirala Bakhshian Bakhshian Nik, Joshua D. Hutchesonduplicate. Circulating Levels of Phosphate Phosphate concentrations were measured using the Phosphate Assay Kit (ab65622, Abcam) according to the manufacturer’s instructions. Serum or plasma samples were diluted 1:500 in

(8) Phosphate Improves Mitochondrial Function and Reduces Pancreatitis in HypertriglyceridemiaFASEB JAugust 26, 2025Nidula Mullappilly, Sandip M. Swain, Joelle M.‐J. Romac, Husam Mikati, Rodger A. Liddleand tissue phosphate were measured using the Phosphate Colorimetric Assay Kit (Abcam, catalog number ab65622) according to the manufacturer's instructions. Measurements for cholesterol, triglycerides, and lipase

(9) Phosphate Improves Mitochondrial Function and Reduces Pancreatitis in HypertriglyceridemiaFASEB JAugust 26, 2025Nidula Mullappilly, Sandip M. Swain, Joelle M.‐J. Romac, Husam Mikati, Rodger A. Liddleand tissue phosphate were measured using the Phosphate Colorimetric Assay Kit (Abcam, catalog number ab65622) according to the manufacturer's instructions. Measurements for cholesterol, triglycerides, and lipase

(10) Klotho Deficiency Promotes Skeletal Muscle Weakness and Is Associated with Impaired Motor Unit Connectivity †Int J Mol SciAugust 19, 2025Linda A. Bean, Connor Thomas, Juan F. Villa, Alexander J. Fitt, Areli Jannes S. Javier, Akanksha Agrawal, Hanna Whitney, Guilherme Nascimento Dos Santos, Kenneth E. White, Joshua R. Huot, et al.USA). 4.5. Serum Analyses Creatine kinase (CK) activity (#MAK116, MilliporeSigma) and phosphate levels (#ab65622, Abcam, Cambridge, MA, USA) were measured in serum using commercially available assays. Absorbance was

(11) Klotho deficiency promotes skeletal muscle weakness and is associated with impaired motor unit connectivitybioRxivJune 19, 2025Linda A. Bean, Connor Thomas, Juan F. Villa, Alexander J. Fitt, Areli Jannes S. Javier, Akanksha Agrawal, Hanna Whitney, Guilherme Nascimento Dos Santos, Kenneth E. White, Joshua R. Huot, et al.CA). Serum Analyses Creatine kinase (CK) activity (#MAK116, MilliporeSigma) and phosphate levels (#ab65622, Abcam, Cambridge, MA) were measured in serum using commercially available assays. Absorbance was read

(12) Characterization of TNSALP expression and activity in porcine utero-placental tissuesReprod FertilJune 5, 2025Claire Stenhouse, Katherine M Halloran, Katherine M Halloran, Makenzie G Newton, Makenzie G Newton, Nirvay Cain, Heewon Cain, Heewon Seo, Gregory A Johnson, Fuller W Bazerof phosphate in allantoic and amniotic fluid were quantified using a colorimetric assay (Abcam, USA; ab65622) as previously described (Stenhouse et al. 2021). In brief, samples and the phosphate standards (200

(13) Characterization of TNSALP expression and activity in porcine utero-placental tissuesReprod FertilJune 5, 2025Claire Stenhouse, Nirvay Sah, Katherine M Halloran, Robyn M Moses, Makenzie G Newton, Joe W Cain, Heewon Seo, Guoyao Wu, Gregory A Johnson, Fuller W Bazerof phosphate in allantoic and amniotic fluid were quantified using a colorimetric assay (Abcam, USA; ab65622) as previously described (Stenhouse et al. 2021). In brief, samples and the phosphate standards (200

(14) Knockout of the V-ATPase interacting protein Tldc2 in B-type kidney intercalated cells impairs urine alkalinizationAmerican Journal of Physiology-Renal PhysiologyJune 1, 2025Amity F. Eaton, Elizabeth C. Danielson, Leona J. Tu, Dennis Brown, Maria Merkulovabefore (27, 28). Spot urine was also analyzed with a commercially available phosphate assay kit (cat # ab65622, Abcam, Waltham, MA), carbon dioxide (liquid) reagent set (cat # C7502120, Pointe Scientific, Canton,

(15) Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidationNat CommunMay 14, 2025Jakob Höppner, Hiroshi Noda, Anju Krishnan Anitha, Ross W. Cheloha, Thomas Dean, Michael Bruce, Daniel J. Brooks, Michael Mannstadt, Mary L. Bouxsein, Samuel H. Gellman, et al.phosphorus (Pi) using a Colorimetric Phosphate Assay Kit (Abcam Waltham, MA 02453, USA; Catalog No. ab65622). Serum or plasma levels of C-terminal telopeptide type I collagen (CTX-1), N-terminal propeptide of

(16) Rescue of the First Mitochondrial Membrane Carrier, the mPiC, by TAT-Mediated Protein Replacement TreatmentInt J Mol SciMay 5, 2025Samar Zabit, Orly Melloul, Michal Lichtenstein, Erin L. Seifert, Haya Lorberboum-Galskithe Abcam protocol [37]. Pi and Cu levels were measured within isolated mitochondria using the Abcam ab65622 Phosphate Assay Kit [38] and the Abcam ab272528 Copper Assay Kit [39], respectively, according to the

(17) Vitamin D Receptor signalling regulates the diet-driven metabolic shift during weaningMolecular MetabolismApril 26, 2025Neha Jawla, Shubhi Khare, Nidhi Yadav, Ranjan Kumar Nanda, G. Aneeshkumar Arimbasseriestimation kit (Abcam, ab102505) and serum phosphate levels were estimated using phosphate kit (Abcam, ab65622) according to manufacturer's instructions. 2.13 Bodipy uptake assay For fatty acid uptake assay, mice

(18) Targeting aldolase A in hepatocellular carcinoma leads to imbalanced glycolysis and energy stress due to uncontrolled FBP accumulationNature MetabolismJanuary 20, 2025Marteinn T. Snaebjornsson, Philipp Poeller, Daria Komkova, Florian Röhrig, Lisa Schlicker, Alina M. Winkelkotte, Adriano B. Chaves-Filho, Kamal M. Al-Shami, Carolina Dehesa Caballero, Ioanna Koltsaki, et al.Information. Phosphate, ATP, ATP/ADP, NAD(P)+/NAD(P)H measurements Pi was measured using phosphate assay kit (ab65622, Abcam) according to the manufacturer’s instructions, results were then normalized to protein content.

(19) Sox10 is required for systemic initiation of bone mineralizationDevelopment (Cambridge, England)January 15, 2025Gjorcheska S, Paudel S, McLeod S, Paulding D, Snape L, Sosa KC, Duan C, Kelsh R, Barske L.content Whole-body Ca2+ and PO43− contents were quantified using colorimetric assay kits (Abcam, ab102505 and ab65622). For Ca2+ measurements, 10-15 larvae were pooled at the desired stage in an Eppendorf tube without

(20) In nondiabetic C57BL/6J mice, canagliflozin affects the skeleton in a sex- and age-dependent mannerJBMR PlusNovember 3, 2024Carolyn Chlebek, Casey McAndrews, Samantha N Costa, Victoria E DeMambro, Shoshana Yakar, Clifford J Rosen(1:2 dilution) and phosphate (1:2000 dilution) were evaluated in the urine (Abcam ab102505 and Abcam ab65622, respectively). Calcium and phosphate levels were normalized to creatinine. Statistical analyses Longitudinal

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Phosphate Assay Kit (Colorimetric) from Abcam

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Phosphate Assay Kit (Colorimetric) from Abcam

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