Fig 1: Hepatic glycogen, triglyceride and gluconeogenic content and expression in PANKO-C57. (A) Hepatic glycogen content (in μg/μl of tissue lysate) measurement of male PANKO-C57 (KO) and WT mice at 4–5 months of age following insulin stimulation (2 U/kg of body weight) or an overnight fast using the Glycogen Assay Kit (Abcam®) (n=3 per group). (B) Hepatic triglyceride content evaluation of 4-to 5-month-old male PANKO-C57 and WT mice as described in A, using 100 mg of liver tissue and the Triglyceride Quantification Kit (Abcam®) (n=3). (C) Serum triglycerides from serum after fasting of PANKO-C57 and WT male mice were analyzed longitudinally at 2 and 5 months of age using the Triglyceride Quantification Kit (Abcam®) (n=5). Values are expressed as the mean+s.e.m. *P<0.05, **P<0.01 as determined by using Student’s t-test.