WST-1 Assay Kit (Cell Proliferation) ab65473 from Abcam

Product Specs
ItemWST-1 Assay Kit (Cell Proliferation)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65473
MethodFuncS
Quantity500 Test, 2500 Test
Sample TypeAdherent cells, Suspension cells
Assay Time4h 0m

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Cambridge Biomedical Campus

Cambridge CB2 0AX

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Nimesulide Containing Dimethoxy Benzamide: From Fever Reduction to Cancer TreatmentACS OmegaDecember 16, 2025Ibrahim Abdulaziz Abdullah Alshimari, Saja Adel Fakhraldeen, Laila A. Jaragh-Alhadadincluding DMEM media, FBS, penicillin/streptomycin, 1% l-glutathione, PBS, and the WST-1 assay kit (ab65473, Abcam), were purchased from Sigma-Aldrich and were ready for direct use. 2.2 Instruments Employed All

(2) Significance of Nano Transition Metal Complexes as Anticancer and Antibacterial Therapeutic AgentsInternational Journal of Molecular SciencesOctober 29, 2025Abdulaziz Alshehab, Ameena Haider, Laila Jaragh-AlhadadCoCl2, ZnCl2, the DMEM medium, FBS, penicillin/streptomycin, 1% L-glutathione, PBS, WST-1 test Kit (ab65473, Abcam, Cambridge, MA, USA), and nutrient agar. All were purchased from Sigma-Aldrich, Germany, and

(3) Empagliflozin Attenuates Neointima Formation After Arterial Injury and Inhibits Smooth Muscle Cell Proliferation and Migration by Suppressing Platelet‐Derived Growth Factor–Related SignalingJournal of the American Heart AssociationNovember 19, 2024Gwo‐Jyh Chang, Wei‐Jan Chen, Yu‐Juei Hsu, Ying‐Hwa Chenrat aortic VSMCs was evaluated using a commercially available CytoScan WST‐1 Cell Cytotoxicity Assay (ab65473; Abcam). Statistical Analysis Data are presented as means±SEM. For comparisons between 2 groups, unpaired

(4) Cell sheet produced from periodontal ligament stem cells activated by PAR1 improves osteogenic differentiationBrazilian Oral ResearchSeptember 2, 2024Letícia Miquelitto GASPARONI, Tomaz ALVES, Bruno Nunes de de FRANÇA, Danilo BALZARINI, Emmanuel ALBUQUERQUE-SOUZA, Ana Clara Fagundes PEDRONI, Emanuel da Silva ROVAI, Aldrin Huamán MENDOZA, Carla Renata SIPERT, Marinella HOLZHAUSENcells/well in triplicate. The proliferation capacity of PDLSCs was analyzed using the WST-1 Assay Kit (ab65473, Abcam, Cambridge, UK), according to manufacturer’s instructions, and absorbance was measured at 450

(5) Modulation of microglia activation by the ascorbic acid transporter SVCT2Brain, Behavior, and ImmunityAugust 1, 2024Amanda L. Marino, Tonia S. Rex, Fiona E. Harrisoncell viability Cell viability was determined using the WST-1 Cell Proliferation Assay (Abcam, USA, #ab65473) and run according to manufacturer’s specifications. Briefly, cells were plated into a 96-well plate

(6) Effects of bone surface topography and chemistry on macrophage polarizationScientific ReportsJune 3, 2024Birgün Özcolak, Berkay Erenay, Sedat Odabaş, Klaus D. Jandt, Bora GaripcanRAW 264.7 cells analyzed via WST-1 assay by using a commercial kit according to the manufacturer’s instructions (ab65473; Abcam, Cambridge, MA) at day 1, day 3, and day 6. Experiments were conducted in triplicates and plain

(7) Effects of high glucose and severe hypoxia on the biological behavior of mesenchymal stem cells at various passagesWorld Journal of Stem CellsApril 26, 2024Fatimah Almahasneh, Ejlal Abu-El-Rub, Ramada R Khasawneh, Rawan Almazariconditions (high glucose and hypoxia) was measured using the commercial kit (WST-1 Assay Kit, Abcam, Cat# ab65473). The WST-1 assay is based on the cleavage of tetrazolium salt WST-1 to formazan by cellular mitochondrial

(8) The effect of continuous long-term illumination with visible light in different spectral ranges on mammalian cellsScientific ReportsApril 24, 2024Sophie Dani, Kathleen Schütz, Ezgi Dikici, Anne Bernhardt, Anja Lodesamples cultivated until day 7. Analysis of metabolic activity The WST-1 cell proliferation kit (ab65473, Abcam, United Kingdom) is based on the cleaving of the tetrazolium salt WST-1 to a soluble formazan

(9) A chemical proteomics approach for global mapping of functional lysines on cell surface of living cellNature CommunicationsApril 8, 2024Ting Wang, Shiyun Ma, Guanghui Ji, Guoli Wang, Yang Liu, Lei Zhang, Ying Zhang, Haojie Lupositive control. The cell viability was assessed using the WST1 (water-soluble tetrazolium salt-1) (ab65473; Abcam) assay according to the manufacturer’s instructions. Membrane permeation assay Hela cells were

(10) Interferon-β Overexpression in Adipose Tissue-Derived Stem Cells Induces HepG2 and Macrophage Cell Death in Liver Tumor Organoids via Induction of TNF-Related Apoptosis-Inducing Ligand ExpressionInternational journal of molecular sciencesJanuary 22, 2024Yoon Y, Kim CW, Kim MY, Baik SK, Jung PY, Eom YW.with IFN-β or TRAIL for 1 day, liver tumor organoid viability was measured using the WST-1 Assay Kit (ab65473; Abcam, Cambridge, UK) according to the manufacturer’s instructions. Briefly, 15 µL of WST-1 reagent

(11) Sphingosine-1-phosphate Treatment Improves Cryopreservation Efficiency in Human Mesenchymal Stem CellsLife (Basel)May 30, 2023Seong-Ju Oh, Chan-Hee Jo, Tae-Seok Kim, Chae-Yeon Hong, Sung-Lim Lee, Young-Hoon Kang, Gyu-Jin Rhoh. Cells were then treated with or without 10 μM S1P. After 1 h of treatment, WST-1 reagent (Abcam, #ab65475) was added to each well, and the cells were incubated for 2 h at 37 °C. The absorbance was measured

(12) SH2 Domain-Containing Phosphatase-SHP2 Attenuates Fibrotic Responses through Negative Regulation of Mitochondrial Metabolism in Lung FibroblastsDiagnostics (Basel, Switzerland)March 18, 2023Karampitsakos T, Galaris A, Barbayianni I, DeIuliis G, Ahangari F, Sampsonas F, Sotiropoulou V, Aidinis V, Bennett AM, Herazo-Maya JD, et al.Abcam, Cambridge, UK (ab). 2.2. Proliferation Assay The Quick Cell Proliferation Assay Kit (Abcam-ab65473) was used to measure primary MLFs from SHP2D61G/+ and SHP2+/+ mice proliferation, as it was previously

(13) Low-density lipoprotein encapsulated thiosemicarbazone metal complexes is active targeting vehicle for breast, lung, and prostate cancersDrug DeliveryDecember 31, 2022Laila Jaragh-Alhadad, Mayada Samir, Terri J. Harford, Sadashiva Karnikin-vitro cytotoxicity assay was carried out based on the manufacturer’s protocol provided by WST-1 assay Kit (ab65473 Abcam, USA). Briefly, 5 × 104 cells/well/ml from the MCF7, A549, and C42 cell lines were seeded on 96-well

(14) Cancer targeted drug delivery using active low-density lipoprotein nanoparticles encapsulated pyrimidines heterocyclic anticancer agents as microtubule inhibitorsDrug DeliveryDecember 31, 2022Laila Jaragh-Alhadad, Haider Behbehani, Sadashiva Karnikin-vitro cytotoxicity assay was carried out based on the manufacturer's protocol provided by WST-1 assay Kit (ab65473 Abcam, USA). Briefly, 5 × 104 cell/well/ml from the breast and prostate cell lines were seeded on 96-well

(15) Acid external and internal environment exchange the Oreochromis niloticus tissue immune gene expression compared to the mouse macrophage polarization modelFrontiers in ImmunologySeptember 26, 2022Po-Kai Pan, Tsung-Meng Wu, Hsin-Yuan Tsai, I-Cheng Cho, Hsin-Wei Tseng, Tai-Du Lin, Fan-Hua Nan, Yu-Sheng Wuend of the incubation time, a cell counting kit-8 (CCK-8) (B34302, Bimake) and WST-1 detection kit (ab65473, Abcam) were used to examine cell survival and proliferation activity by measuring cellular dehydrogenase

(16) p53 as a unique target of action of cisplatin in acute leukaemia cellsJournal of Cellular and Molecular MedicineSeptember 1, 2022Sanjay Kumar, Paul B. Tchounwouculture medium for 48 h. After incubation, WST cell proliferation was performed by Abcam Company (Cat # ab65473) instruction manual. In brief, after cisplatin treatment, 10‐μl WST/ECS solution was added to each well

(17) Immunostimulatory activity of soybean hull polysaccharide on macrophagesExperimental and Therapeutic MedicineApril 12, 2022Mengxue Wang, Chenye Fu, Mingcong Zhang, Yuxian Zhang, Liang Caowere determined using the Griess reaction and water-soluble tetrazolium-1 (WST-1) assays (cat. no. ab65475; Abcam), respectively, according to the manufacturer's instructions. The optical density was measured

(18) Precision analysis of mutant U2AF1 activity reveals deployment of stress granules in myeloid malignanciesMolecular cellMarch 17, 2022Biancon G, Joshi P, Zimmer JT, Hunck T, Gao Y, Lessard MD, Courchaine E, Barentine AES, Machyna M, Botti V, et al.viability measurements were obtained by flow cytometry and by colorimetric WST-1 assay (Abcam, Cat #ab65475). For flow cytometry analysis, cells were stained with 1.5 μl 7-aminoactinomycin D (7-AAD) solution

(19) Nitro-Deficient Niclosamide Confers Reduced Genotoxicity and Retains Mitochondrial Uncoupling Activity for Cancer TherapyInternational journal of molecular sciencesSeptember 27, 2021Ngai TW, Elfar GA, Yeo P, Phua N, Hor JH, Chen S, Ho YS, Cheok CF.Cell Proliferation Assay Cell viability was measured using WST-1 Cell Proliferation Assay (Abcam, USA ab65473). A total of 1500 cells were seeded onto a 96-well plate and were seated for 24 h before drug treatment

(20) PTHrP Drives Pancreatic Cancer Growth and Metastasis and Reveals a New Therapeutic VulnerabilityCancer discoveryJuly 1, 2021Pitarresi JR, Norgard RJ, Chiarella AM, Suzuki K, Bakir B, Sahu V, Li J, Zhao J, Marchand B, Wengyn MD, et al.RNA isolated for downstream analysis. Cell viability assays WST-1 Cell Proliferation Assay (Abcam ab65475) was performed per the manufacturer’s instructions. Briefly, 2.0×103-5.0×103 cells were plated on 96-well

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WST-1 Assay Kit (Cell Proliferation) from Abcam

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WST-1 Assay Kit (Cell Proliferation) from Abcam

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