Acid Phosphatase Assay Kit (Colorimetric) ab83367 from Abcam

Product Specs
ItemAcid Phosphatase Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab83367
Quantity500 Test
MethodFuncS
Sample TypeUrine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
SensitivityMore than 0.001 mM
Assay Time1h 10m
ApplicationsEnzyme activity assay
Gene NameACP1
Detection Range0.001 - 1 mM
NCBI Gene AliasesLAP
NCBI Full Gene Nameacid phosphatase 2, lysosomal

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) c-Jun N-terminal Kinase Supports Autophagy in Testicular Ischemia but Triggers Apoptosis in Ischemia-Reperfusion InjuryInternational Journal of Molecular SciencesSeptember 27, 2024Sarah R. Alotaibi, Waleed M. Renno, May Al-MaghrebiBiochemical Assays 4.7.1. Alkaline Phosphatase Activity The colorimetric acid phosphatase assay kit (Ab83367; Abcam; Cambridge, UK) was used to determine the enzymatic activity of acid phosphatase (AP) in 350

(2) BAP1 Deficiency Inflames the Tumor Immune Microenvironment and Is a Candidate Biomarker for Immunotherapy Response in Malignant Pleural MesotheliomaJTO Clinical and Research ReportsMay 1, 2024Duo Xu, Yanyun Gao, Haitang Yang, Marc Spils, Thomas M. Marti, Tereza Losmanová, Min Su, Wenxiang Wang, Qinghua Zhou, Patrick Dorn, et al.drug treatment for up to 5 days. The cell number was then determined by Acid Phosphatase Assay Kit (ab83367; Abcam), and the reported “cell change %” was determined as follows: 100 × (Value-Time 0)/ (Baseline-Time

(3) Photoactive Poly-L-Lysine gel with resveratrol-magnesium metal polyphenol network: A promising strategy for preventing tracheal anastomotic complications following surgeryMaterials Today BioFebruary 1, 2024Yunxuan Jia, Jingfeng Shi, Bowen Ding, Liang Zhao, Ke Xu, Chuang Hu, Weijiao Xu, Anshun Zhu, Haitang Yang, Xiansong Wang, et al.24h. After 72h of drug treatment, viability was assessed using the Alkaline Phosphatase Assay Kit (ab83367; Abcam, Cambridge, UK), as we previously described [[41], [42], [43], [44]]. For the colony formation

(4) Effects of empagliflozin on gonadal functions of hyperglycemic male wistar ratsPloS oneJanuary 1, 2024Bdeir R, Al-Sawalha NA, Al-Fawares O, Hamadeneh L, Khawaldeh A.concentration in each sample. Assay of testicular enzymes Acid phosphatase (ACP) (Abcam, USA, cat. # ab83367), glucose-6-phosphate dehydrogenase (G6PD) (MyBiosource, USA, cat. # MBS2702342), sorbitol dehydrogenase (SDH

(5) MEK1 drives oncogenic signaling and interacts with PARP1 for genomic and metabolic homeostasis in malignant pleural mesotheliomaCell death discoveryFebruary 10, 2023Yang H, Gao Y, Xu D, Xu K, Liang SQ, Yang Z, Scherz A, Hall SRR, Forster S, Berezowska S, et al.(1000–1500 cells/well) were treated with drugs 24 h later and assayed by Acid Phosphatase Assay Kit (ab83367; Abcam). Drug synergy was determined by CompuSyn software (www.combosyn.com) to calculate combination

(6) Hypoxia modulates human mast cell adhesion to hyaluronic acidImmunologic ResearchApril 1, 2022Joanna Pastwińska, Aurelia Walczak-Drzewiecka, Elżbieta Kozłowska, Enjuro Harunari, Marcin Ratajewski, Jarosław Dastychadhesion. The percentage of adherent cells was determined using the Acid Phosphatase Assay Kit (Abcam, ab83367). We slightly modified the protocol to adapt the assay to 96-well plates instead of using separate tubes.

(7) Pharmaco-transcriptomic correlation analysis reveals novel responsive signatures to HDAC inhibitors and identifies Dasatinib as a synergistic interactor in small-cell lung cancereBioMedicineJuly 3, 2021Haitang Yang, Beibei Sun, Ke Xu, Yunfei He, Tuo Zhang, Sean R R Hall, Swee T. Tan, Ralph A. Schmid, Ren-Wang Peng, Guohong Hu, et al.alone or in combination for 96 h, and cell viability was quantified by the Acid Phosphatase Assay Kit (ab83367; Abcam, Cambridge, UK) according to the manufacturer's protocol. The median inhibitory concentration

(8) Systematic Analysis of Aberrant Biochemical Networks and Potential Drug Vulnerabilities Induced by Tumor Suppressor Loss in Malignant Pleural MesotheliomaCancers (Basel)August 17, 2020Haitang Yang, Duo Xu, Zhang Yang, Feng Yao, Heng Zhao, Ralph A. Schmid, Ren-Wang PengDMSO as vehicle. Cell viability was determined 72 h post-treatment by the Acid Phosphatase Assay Kit (ab83367; Abcam) [68]. The median inhibitory concentration (IC50) was calculated using GraphPad Prism 7. 4.3.

(9) Hypoxia regulates human mast cell adhesion to fibronectin via the PI3K/AKT signaling pathwayCell Adhesion & MigrationMay 20, 2020Joanna Pastwińska, Aurelia Walczak-Drzewiecka, Magdalena Łukasiak, Marcin Ratajewski, Jarosław Dastychadhesion. To determine the percentage of adherent cells, we modified the Acid Phosphatase Assay Kit (Abcam, ab83367) protocol. The principle of the test is to measure the activity of acid phosphatase, which is an enzyme

(10) HSP90/AXL/eIF4E-regulated unfolded protein response as an acquired vulnerability in drug-resistant KRAS-mutant lung cancerOncogenesisAugust 20, 2019Yang H, Liang SQ, Xu D, Yang Z, Marti TM, Gao Y, Kocher GJ, Zhao H, Schmid RA, Peng RW.as indicated. Cell viability was determined 72 h post-treatment by the Acid Phosphatase Assay Kit (ab83367; Abcam, Cambridge, UK) according to the manufacturer’s protocol. Drug synergism was determined by CompuSyn

(11) Identification of a factor controlling lysosomal homeostasis using a novel lysosomal trafficking probeScientific reportsAugust 12, 2019Ishii S, Matsuura A, Itakura E.an Acid Phosphatase Assay Kit (Colorimetric) according to the manufacturer’s instructions (Cat No. ab83367, Abcam, Cambridge, UK). The acid phosphatase activity was normalised to the protein concentration. RNA

(12) NF2 and Canonical Hippo-YAP Pathway Define Distinct Tumor Subsets Characterized by Different Immune Deficiency and Treatment Implications in Human Pleural MesotheliomaCancersJanuary 1, 0001"Yang H, Hall SRR, Sun B, Zhao L, Gao Y, Schmid RA, Tan ST, Peng RW, Yao F."as the vehicle. Cell viability was determined 96 h post-treatment by the Acid Phosphatase Assay Kit (ab83367; Abcam) [9,84]. The median inhibitory concentration (IC50) was calculated using GraphPad Prism 8. 4.3.

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Acid Phosphatase Assay Kit (Colorimetric) from Abcam

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Acid Phosphatase Assay Kit (Colorimetric) from Abcam

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