LDH Assay Kit (Cytotoxicity) ab65393 from Abcam

Product Specs
ItemLDH Assay Kit (Cytotoxicity)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65393
Quantity500 Test, 10000 Test
MethodFuncS
Sample TypeAdherent cells, Suspension cells
Assay Time1h 0m
Gene NameldhA
NCBI Gene AliasesGSD11, HEL-S-133P, LDHM, PIG19
Research AreaMetabolism
NCBI Full Gene Namelactate dehydrogenase A

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Abcam

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Phospho-JNK agonists show promising effects for the treatment of hepatocellular carcinomaiScienceJune 19, 2026Woonghee Kim, Han Jin, Peipei Miao, Mehmet Ozcan, Xinmeng Liao, Mengzhen Li, Shazia Iqbal, Jihad Sebhaoui, Sajda Ashraf, Burcu Belmen, et al.viability was measured by MTT assay (M6494, ThermoFisher) and cytotoxicity was measured by LDH assay kit (ab65393, Abcam) by following the manufacturer’s instructions. Optical density (O.D) had been enumerated with

(2) Angiotensin-(1–7) suppresses pyroptosis in cerebral endothelium to decrease blood–brain barrier permeability and cognitive impairments in sepsisJournal of International Medical ResearchApril 29, 2026Yongli Han, Hongguang Ding, Yirun Chen, Yin Wen, Huishan Zhu, Zhiwei Su, Jiaqi Tang, Hongke Zengdetection Pyroptosis assessment was performed via measurement of LDH release, utilizing a commercial assay kit (ab65393, Abcam). Following the treatment, the culture supernatant was collected and subsequently centrifuged

(3) Targeting the noncanonical function of metabolic enzyme PHGDH in driving PD-L1 expression and cancer immune evasionCell Reports MedicineApril 21, 2026Juan Liu, Weiwei Wang, Yvonne Sun, Shan Huang, Arnav Borole, Haiyan Zheng, Wenwei Hu, Zhaohui FengKit,Abcam,Cat# ab273328 Tumor Dissociation Kit,Miltenyi Biotec,Cat# 130-096-730 LDH release assay,Abcam,Cat# ab65393 CD8+ T cell isolation Kit,Miltenyi Biotec,Cat# 130-104-075 MycoAlert mycoplasma detection kit,Lonza

(4) Development of Styryl-Modified 3,4-Dihydropyrimidin-2(1H)-ones as Potential Antitumor AgentsChemMedChemApril 14, 2026Konstantinos Panagoulias, Woonghee Kim, Murat Ozdemir, Busra Turan, Adil Mardinoglu, Hasan Turkez, Daniela Trisciuzzi, Orazio Nicolotti, Antonio Di Stefano, Stamatia Vassiliou, et al.viability was measured by MTT assay (M6494, ThermoFisher) and cytotoxicity was measured by LDH assay kit (ab65393, Abcam) by following the manufacturer's instructions. Optical density (O.D) had been enumerated with

(5) Systematic Comparison of the TGF-β Isoforms in Normal Dermal and Lung Fibroblasts Identifies TGF-β2 and TGF-β3 as Priority Targets in Tissue FibrosisCellsApril 10, 2026Raveen Badyal, Brandon Kohlen, Kevin J. Keen, James V. Dunne, Tillie-Louise HackettLactate dehydrogenase (LDH) release by fibroblasts was measured with an LDH assay kit from Abcam (Cat: ab65393) according to the manufacturer’s protocol. Experiments were conducted using 10 biological replicates

(6) Electrospun CHIR99021-loaded cellulose acetate scaffolds stimulate osteogenic differentiation and mineralization in vitroJournal of Materials Science: Materials in MedicineApril 3, 2026Samir A. Bello, Aimeé Rodríguez-Monroig, Andrea Vargas-Morales, Alondra S. Rodríguez -Rolón, Claudia C. Zuluaga-Gómez, Dina Paola Bracho-Rincón, Eduardo Nicolauobtained from Millipore-Sigma (Temecula, CA). The alkaline phosphatase Assay (ab83369), LDH cytotoxicity (ab65393), and BCA protein quantification (ab1025360) kits were procured from Abcam (Waltham, MA). Secondary

(7) Tissue and Isoform-Specific Effects of Platelet-Derived Growth Factor on Neonatal-Derived Dermal and Fetal-Derived Lung Fibroblast Profibrotic FunctionsCellsApril 1, 2026Brandon Kohlen, Raveen Badyal, Kevin J. Keen, James V. Dunne, Tillie-Louise HackettLactate Dehydrogenase (LDH) Cytotoxicity Assay Cell death was quantified using the LDH assay kit (cat. ab65393) with a standard curve of recombinant human lactate dehydrogenase protein (cat. ab93699), both sourced

(8) Berry e-liquid flavour toxicities are not equal on an alveolar-capillary barrier cell model according to two exposure methodsFront ToxicolMarch 11, 2026Emma Filaudeau, Amelia-Naomi Sabo, Anita Lebert, Laurent Monassier, Véronique KemmelHydrocortisone (#H0888) was obtained from Sigma (Saint-Louis, United States). Lactate Dehydrogenase (LDH) kit (#ab65393), MTS kit (#ab197010) and SP-D SingleStep ELISA® kit (#ab239431) were acquired at Abcam (Cambridge,

(9) Food-derived β-lactoglobulin nanofibrils: An efficacy, safe, and scalable solution to overcome oral insulin delivery challengesBioactive MaterialsMarch 1, 2026Xihua Liu, Shuangjian Li, Guodong Wu, Wenzhe Jia, Yiguo Zhao, Yapeng Fang, Yiping CaoLDH release (i.e., cell membrane damage) was determined using the colorimetric LDH Assay Kit (Abcam, ab65393). 4.23 Intracellular Ca2+ imaging Intracellular calcium dynamics were monitored using fluorescent

(10) Extracellular Vesicles from Probiotic and Beneficial Escherichia coli Strains Exert Multifaceted Protective Effects Against Rotavirus Infection in Intestinal Epithelial CellsPharmaceuticsJanuary 18, 2026Cecilia Cordero, Aitor Caballero-Román, Sergio Martínez-Ruiz, Yenifer Olivo-Martínez, Laura Baldoma, Josefa BadiaCarlsbad, CA, USA). Cytotoxic effects were assessed using the LDH-Cytotoxicity Assay Kit II (Cat. No. ab65393, Abcam, Cambridge, UK), according to the manufacturer’s instructions. This assay quantifies lactate

(11) Optimization and Characterization of SHIP1 Ligands for Cellular Target Engagement and Activity in Alzheimer’s Disease ModelsbioRxivJanuary 5, 2026Cynthia D. Jesudason, Claudia Rangel-Barajas, Colin J. Beach, Daniel E. Beck, Isaac H. Caballero-Floran, W. Brent Clayton, Lais Da Silva, Juliane C. David, Suzanne Doolen, Alexandra N. Faulkner, et al.culture supernatant was used as a measure of cytotoxicity81. The LDH Cytotoxicity Assay Kit (Abcam, ab65393) was used according to the manufacturer’s instructions. In brief, 10 μL of culture supernatant was combined

(12) Streptococcus gordonii type VII secretion system substrate EsxA induces neutrophil extracellular trap formation in infective endocarditisSci RepDecember 12, 2025Chih-Chieh Hsu, Yu-Chung Chuang, Ron-Bin Hsu, Jeng-Wei Chen, Jean-San Chia, Yi-Hsien Shih, Yu-Min Kuo, Chiau-Jing Junginfection (MOI) of 100 and incubated for 8 h. LDH release was then quantified using an LDH assay kit (Abcam ab65393) according to the manufacturer’s instructions. Transwell cytotoxicity assays were performed in 24-well

(13) Comprehensive multi-omics mapping of immune perturbations in autism spectrum disorderClin Transl MedDecember 12, 2025Chun Yan, Fangmei Feng, Chaoting Lan, Gang Luo, Xiaotao Jiang, Huijuan Wang, Yinchun Chen, Yuling Yang, Liangqiong Deng, Xiaoli Huang, et al.assay LDH cytotoxicity assay was conducted with a commercial LDH Cytotoxicity Assay Kit (Abcam, Cat#ab65393) following the supplier's protocol. K562 cells (2 × 104 cells/well) were plated in 48‐well plates and

(14) Sulphonamide derivatives with a triazine core as novel inducers of apoptosis and pyroptosis in glioblastoma multiforme cellsJ Enzyme Inhib Med ChemDecember 4, 2025Olga Klaudia Szewczyk-Roszczenko, Katarzyna Kotwica-Mojzych, Piotr Roszczenko, Mariusz Mojzych, Adolfo Rivero-Müller, Karolina Czapla, Alicja Przybyszewska-Podstawka, Krzysztof Bielawski, Robert CzarnomysyA172 and U-87 MG cells were cultured and plated in 96-well plates. The LDH-Cytotoxicity Assay Kit II (ab65393) (Abcam, Cambridge, UK) was used to assess cytotoxicity according to the manufacturer’s protocol. The

(15) FGF2 Attenuated Inflammation-Mediated Cardiac Damage: A Novel Mechanistic Insight into the AMPK–FUNDC1–MitophagyResearch (Wash D C)November 24, 2025Pingjun Zhu, Xi Wang, Xinjie Han, Yan Wang, Yongkai Ding, Min Zhu, Xin Zhang, Qian Xu, Yukun Li, Zhongxuan Li, et al.release, an indicator of cell membrane integrity and cytotoxicity, was measured using the LDH Assay Kit (ab65393, Abcam, Cambridge, UK) following the manufacturer’s protocol [46]. The MTT and CCK-8 assays were conducted

(16) CD155-based chimeric antigen receptor T cells: a promising immunotherapy for cervical and breast cancerFrontiers in ImmunologyNovember 19, 2025Jun Ma, Wenjing Zhu, Rui Zhao, Qianqian Shi, Fang Yang, Yangnan Ding, Enwu Yuan, Kai Zhang, Xin Zhaocytotoxicity of the CAR T cells was assessed using the lactic dehydrogenase (LDH) release assay (no. ab65393; Abcam, Cambridge, UK) according to the manufacturer’s instructions. The cells were plated in 96-well

(17) Human coronavirus OC43 infection in human cerebral organoids: novel insights on pathogenesis and potential therapeutic interventionsJournal of Biomedical ScienceNovember 5, 2025Juntong Liu, Yao Deng, Weibang Huo, Jingdong Song, Chengcheng Zhai, Lan Wei, Changcheng Wu, Gaoqian Zhang, Baoying Huang, Wenling Wang, et al.method was used for cell viability detection. The LDH-Cytotoxicity Assay Kit was purchased from Abcam (ab65393), and the tests were conducted in accordance with the manufacturer's instructions. Transmission electron

(18) Endothelial cell-derived apoptotic bodies modulate innate and adaptive immune responses during inflammationCell Commun SignalOctober 7, 2025Caitlin L. Vella, Pamali Fonseka, Emma J. Grant, Stephanie F. Rutter, Donia Y. Abeid, Dilara C. Ozkocak, Tien K. Nguyen, Antony Vinh, Stephanie Paone, Grant R. Drummond, et al.chamber. LDH cytotoxicity assay ApoBD lysis was measured using a lactate dehydrogenase kit (LDH, Abcam, cat #ab65393). Following manufacturer’s instructions, 10 µl of supernatant was added to the LDH reaction mix and

(19) Immunometabolic defects of CD8+ T cells disrupt gut barrier integrity in people with HIVCellOctober 2, 2025Upasana Das Adhikari, Leah M. Froehle, Alexandra N. Pipkin, Heeva Baharlou, Alice H. Linder, Palak Shah, Amanda Hussey, Qiming Zhang, Sarah Nyquist, Saleh Khawaled, et al.,StemCell Technologies,Cat# 17953 Human FABP2/I-FABP DuoSet ELISA,Bio techne,Cat# DY3078 LDH Assay Kit,Abcam,Cat# ab65393 Qubit dsDNA HS Assay Kit,Life Technologies,Cat# Q32851 Nextera XT DNA Library Preparation Kit,Illumina,Cat#

(20) OGT-mediated O-GlcNAc of FOXO1 promotes the progression of neonatal heart failure via regulating pyroptosisScientific ReportsSeptember 30, 2025Juan Wu, Zhiliang Xu, Xiaoou Li, Bing He, Qingyan Zhaowere seeded on 96-well plates, followed by centrifugation to collect the supernatants. LDH assay kit (#ab65393) was acquired from Abcam and utilized to assess the release of LDH in the supernatant of the cell culture medium

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2/11/2022Sensitive and Easy Assay to Detect Cytotoxicity Helena AiresThis cytotoxicity assay enables the analysis of cytotoxicity caused by drugs or compounds without complicated and time-consuming steps.

LDH Assay Kit (Cytotoxicity) from Abcam

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LDH Assay Kit (Cytotoxicity) from Abcam

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