Fig 1: Laser captured iAβ-burdened neurons have increased expression of genes related to oxidative stress and DNA damage response.a Representative image of McSA1 (Aβ) immunoreactivity in Tg hippocampus. b High magnification McSA1 immunoreactivity in subiculum (I) and CA1 (II) in Tg rats as from A. c Schematic depicting laser capture microdissection of CA1 and subiculum neurons from Tg Aβ-burdened neurons and Wt neurons not burdened with Aβ. d Differentially expressed genes including Ercc2, Fancc, Sod2, Gsr (GR), and Idh1 in Aβ-burdened Tg hippocampal neurons as compared to Wt neurons. Fold changes were normalized to Wt expression. Scale bars represent 500 µm in A, 50 µm in (b). n = 5–9 (Wt), n = 6–8 (Tg). Error bars indicate SD. two-tailed t-test, *p < 0.05, **p < 0.01.
Fig 2: Quantitative Analysis of Senescence Markers in H₂O₂-Induced hESC Cells. A SA-β-gal staining revealed a higher proportion of SA-β-gal-positive cells in the H₂O₂-treated group (38.5 ± 4.8%) compared to the control group (10.8 ± 1.7%, p < 0.001). B Immunofluorescence quantitative analysis showed increased relative fluorescence intensity (RFU, normalized to control = 1.0) for p16^INK4a (2.9 ± 0.3-fold, p < 0.01), p21 (2.6 ± 0.2-fold, p < 0.01), UCP2 (3.4 ± 0.4-fold, p < 0.001), and GSR (2.8 ± 0.3-fold, p < 0.001) in the H₂O₂-treated group. C GSR enzyme activity assay indicated higher activity in the H₂O₂-treated group (16.8 ± 1.2 nmol/min/mg) than in the control group (5.4 ± 0.5 nmol/min/mg, p < 0.001)
Fig 3: Immune infiltration analysis of dataset GSE165004. A Bar graph showing the abundance of each immune cell. B Bar graph showing the comparison of the proportion of each immune cell between the 2 groups. C Heatmap demonstrating the correlation of GSR and UCP2 with each immune cell. *, p < 0.05; **, p < 0.01
Fig 4: Identification and validation of CSA-signature genes. A Venn diagram visualizing the acquisition of CSA-signature genes. B In the test set dataset GSE165004, the mRNA expression ratios of UCP2 and GSR in the experimental group were significantly higher than that of the control group. C ROC curves suggesting that UCP2 and GSR have good diagnostic performance. D-E The above results were validated in the external validation dataset GSE26787, and similar results were obtained
Supplier Page from Abcam for Glutathione Reductase (GR) Assay Kit