Branched Chain Amino Acid Assay Kit / BCAA Assay Kit ab83374 from Abcam

Product Specs
ItemBranched Chain Amino Acid Assay Kit / BCAA Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab83374
Quantity100 Test
MethodFuncS
Sample TypeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids
Assay Time0h 40m
SensitivityMore than 0.2 nmol/well

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) IDH3A Deficiency Compromises Adaptive Thermogenesis and Exacerbates Obesity-Induced Metabolic Dysfunction via Impaired BCKDHA-Dependent BCAA CatabolismDiabetesMay 1, 2026Xuhong Lu, Ningning Bai, Jialu Lyu, Jingjing Sun, Yingying Su, Yafen Ye, Tingting Hu, Tianwen Liu, Wenfei Li, Xiaoya Li, et al.via ELISA (ImmunoDiagnostics, Ltd.). Metabolite levels were quantified using a commercial kit (BCAA, ab83374; Abcam) or ultra-performance liquid chromatography–tandem mass spectrometry (Metabo-Profile Biotechnology

(2) Limosilactobacillus reuteri ATG-F4 Ameliorates Dexamethasone-Induced Muscle Atrophy through Modulation of Gut MicrobiotaJ Microbiol BiotechnolMarch 13, 2026Daeyoung Lee, Young-Sil Lee, Gun-Seok Park, Juyi Park, Miji Shin, Hyun-Ja Ko, Jihee Kang(AB SCIEX). BCAA Analysis BCAA levels in the QF muscle tissue were measured using a BCAA Assay Kit (ab83374, Abcam). Flash-frozen QF tissue was homogenized in the provided assay buffer, centrifuged at 15,000

(3) ZNF33A activates Wnt/β-catenin signaling and promotes BCAA catabolism in colorectal cancerCell Communication and SignalingMarch 9, 2026Weihua Yu, Jionghuang Chen, Xiaofan Pu, Hongdan Shen, Liping Cao, Linghua Zhu, Haiping Pei, Xihua Linanalyzed by qPCR. Measurement of BCAAs The branched chain amino acid assay kit was purchased from Abcam (ab83374), and experiments were performed according to the instructions. In brief, cells (4 × 106) were homogenized

(4) Circulating BCKAs are closely associated with adipose tissue insulin resistance in type 2 diabetesDiabetol Metab SyndrAugust 1, 2025Jie Gao, Mai Re YanMu Rouzi, Haofan Yang, Wenhao Zheng, Liang Wang, Tao Lei, Jun Luusing the glucose oxidase method (Roche Diagnostics GmbH, Mannheim, Germany). Plasma BCAAs (Abcam, ab83374, USA) and BCKAs (Fusheng Biologicals, A137748, Shanghai, China) were quantified using enzyme-linked immunosorbent assay

(5) Short-term Physical Activity Reduces Metabolic-associated Steatohepatitis by Promoting the Degradation of Branched-chain Amino Acids in Skeletal MuscleJournal of Clinical and Translational HepatologyJuly 28, 2025Mingshu Gao, Jiaying Li, Yanan Zhang, Jiangtao Huang, Jiaqi Chen, Dawen Liao, Shengnan He, Qian Bi, Lele Ji, Yulu Ducontrol. Quantification of BCAAs BCAA levels were measured using a branched-chain amino acid assay kit (ab83374; Abcam, Cambridge, United Kingdom), following the manufacturer’s instructions.29,30 The enzymatic reaction

(6) Association Between Thyroid Function Parameters and Plasma Branched-Chain Amino and Keto Acids in Patients With Euthyroid Type 2 Diabetes MellitusJ Diabetes ResJuly 2, 2025Xinghua Cai, Yuanying Xu, Jie Gao, Huihui Zhang, Jun Lu, Tao LeiEnzyme-linked immunosorbent assay (ELISA) kits were utilized to detect BCAAs (supplier: Abcam, catalog number: ab83374, sensitivity: 0.2 nmol/well) and BCKAs (supplier: Fu Sheng Biologicals, China, catalog number: A137748,

(7) Short-term Physical Activity Reduces Metabolic-associated Steatohepatitis by Promoting the Degradation of Branched-chain Amino Acids in Skeletal MuscleJ Clin Transl HepatolMay 30, 2025Mingshu Gao, Jiaying Li, Yanan Zhang, Jiangtao Huang, Jiaqi Chen, Dawen Liao, Shengnan He, Qian Bi, Lele Ji, Yulu Ducontrol. Quantification of BCAAs BCAA levels were measured using a branched-chain amino acid assay kit (ab83374; Abcam, Cambridge, United Kingdom), following the manufacturer’s instructions.29,30 The enzymatic reaction

(8) BCAT2 binding to PCBP1 regulates the PI3K/AKT signaling pathway to inhibit autophagy-related apoptosis and ferroptosis in prostate cancerCell Death DisApril 24, 2025Wangli Mei, Mengyu Wei, Chaozhi Tang, Weiyi Li, Bowen Ye, Shiyong Xin, Weiguo Ma, Lin Yeprostate specific antigen levels, tumor stage, biochemical recurrence, etc.). BCCA assay BCAA kits (ab83374; Abcam Limited, Cambridge, UK) were used to detect BCAA levels in the samples. In brief, samples and

(9) Dietary Branched‐Chain Amino Acids Modify Postinfarct Cardiac Remodeling and Function in the Murine HeartJournal of the American Heart AssociationFebruary 18, 2025Daniel C. Nguyen, Collin K. Wells, Madison S. Taylor, Yania Martinez‐Ondaro, Richa Singhal, Kenneth R. Brittian, Robert E. Brainard, Joseph B. Moore, Bradford G. Hillfraction. Plasma BCAA levels were then measured using a commercially available BCAA assay kit (Abcam; ab83374), according to manufacturer's protocol. To measure intracardiac levels of BCAAs, a reverse‐phase liquid chromatography

(10) Dietary Branched‐Chain Amino Acids Modify Postinfarct Cardiac Remodeling and Function in the Murine HeartJ Am Heart AssocFebruary 14, 2025Daniel C. Nguyen, Collin K. Wells, Madison S. Taylor, Yania Martinez‐Ondaro, Richa Singhal, Kenneth R. Brittian, Robert E. Brainard, Joseph B. Moore, Bradford G. Hillfraction. Plasma BCAA levels were then measured using a commercially available BCAA assay kit (Abcam; ab83374), according to manufacturer's protocol. To measure intracardiac levels of BCAAs, a reverse‐phase liquid chromatography

(11) Molecular Turn in Yersinia pestis Pathogenesis: Implications of the gppA Frameshift for Bacterial Survival in Human MacrophageEmerging Microbes & InfectionsFebruary 13, 2025Hongyan Chen, Shiyang Cao, Yazhou Zhou, Tong Wang, Yang Jiao, Yafang Tan, Yarong Wu, Yifan Ren, Yajun Song, Jing-Ren Zhang, et al.h. The levels of BCAAs within Y. pestis were assessed using the Branched Chain Amino Acid Assay Kit (ab83374, Abcam, Boston, MA), following the manufacturer's guidelines. Specifically, bacterial cells (1 × 108)

(12) Gut Microbiome and Metabolome Changes in Chronic Low Back Pain Patients With Vertebral Bone Marrow LesionsJOR SpineJanuary 27, 2025Wentian Li, Ji Tu, Jinjian Zheng, Abhirup Das, Qi Yan, Xiaotao Jiang, Wenyuan Ding, Xupeng Bai, Kaitao Lai, Sidong Yang, et al.Amino Acid Assay Following the manufacturer's instructions, a commercial colorimetric measurement kit (ab83374, Abcam, United Kingdom) was utilized to quantify the BCAA levels in serum and cell culture media samples.

(13) Ubiquitin regulatory X (UBX) domain-containing protein 6 is essential for autophagy induction and inflammation control in macrophagesCellular & molecular immunologyDecember 1, 2024Kim YJ, Lee SG, Park SY, Jeon SM, Kim SI, Kim KT, Roh T, Lee SH, Lee MJ, Lee J, et al.vehicle or LPS (100 ng/mL) for the indicated times. BCAAs were extracted via a BCAA assay kit (Abcam, ab83374) according to the manufacturer’s instructions, and the optical density (OD) at 450 nm was measured via

(14) RNA-binding protein YBX3 promotes PPARγ-SLC3A2 mediated BCAA metabolism fueling brown adipogenesis and thermogenesisMolecular metabolismDecember 1, 2024Chen LY, Wang LW, Wen J, Cao JD, Zhou R, Yang JL, Xiao Y, Su T, Huang Y, Guo Q, et al.the indicated time points, and total plasma BCAA levels were measured using a commercial kit (Abcam, ab83374). 2.6 Serum parameters measurement Serum parameters including serum triglyceride (TG, Elabscience,

(15) Oligonol®, an Oligomerized Polyphenol from Litchi chinensis, Enhances Branched-Chain Amino Acid Transportation and Catabolism to Alleviate SarcopeniaInternational Journal of Molecular SciencesOctober 27, 2024Yun-Ching Chang, Yu-Chi Chen, Yin-Ching Chan, Cheng Liu, Sue-Joan ChangConcentrations BCAA concentrations in the gastrocnemius and blood serum were measured by using the BCAA Assay Kit (ab83374; Abcam, Cambridge, UK), following the manufacturer’s guidelines. Absorbance readings were taken at a

(16) DBT is a metabolic switch for maintenance of proteostasis under proteasomal impairmentElifeSeptember 10, 2024Ran-Der Hwang, YuNing Lu, Qing Tang, Goran Periz, Giho Park, Xiangning Li, Qiwang Xiang, Yang Liu, Tao Zhang, Jiou WangThermo Fisher). BCAA measurement Intracellular levels of BCAAs were analyzed using the BCAA Assay Kit (ab83374, Abcam) based on a colorimetric assay measuring the concentrations of molecules specifically derived

(17) Dietary Branched Chain Amino Acids Modify Post-Infarct Cardiac Remodeling and Function in the Murine HeartbioRxivJuly 23, 2024Daniel C. Nguyen, Collin K. Wells, Madison S. Taylor, Yania Martinez-Ondaro, Kenneth R. Brittian, Robert E. Brainard, Joseph B. Moore Iv, Bradford G. Hillfraction. Plasma BCAA levels were then measured using a commercially available BCAA assay kit (Abcam; ab83374), according to manufacturer’s protocol. Primary Cardiac Fibroblast Isolation and Culture: Cardiac

(18) The YBX3 RNA-binding protein post-transcriptionally controls SLC1A5 mRNA in proliferating and differentiating skeletal muscle cellsJournal of Biological ChemistryFebruary 1, 2024Silina Awad, William Skipper, William Vostrejs, Kendall Ozorowski, Kristen Min, Liva Pfuhler, Darshan Mehta, Amy Cookethe Bioassay Systems EnzyChrom Glutamine Assay Kit (EGLN-100, BioAssay Systems), Abcam BCAA Kit (cat# ab83374, ABCAM) and the Abcam L-Alanine Assay Kit (cat# ab83394, ABCAM) according to the manufacturer’s recommendations

(19) High doses of rosuvastatin induce impaired branched‐chain amino acid catabolism and lead to insulin resistanceExperimental PhysiologyJuly 1, 2023Xue Bai, Xingzhen Long, Fang Song, Baolin Chen, Changcheng Sheng, Cailin Tang, Li Li, Jiaxing Zhang, Rui Zhang, Jiquan Zhang, et al.content in the serum and culture supernatant samples was measured using a BCAA assay kit (cat. no. ab83374, Abcam) according to the manufacturer's protocol. The kit utilizes an enzyme assay in which BCAAs are

(20) Progressive Cardiac Metabolic Defects Accompany Diastolic and Severe Systolic Dysfunction in Spontaneously Hypertensive Rat HeartsJournal of the American Heart AssociationMay 16, 2023Li J, Minczuk K, Huang Q, Kemp BA, Howell NL, Chordia MD, Roy RJ, Patrie JT, Qureshi Z, Kramer CM, et al.NEFA‐HR2 kit (Wako), insulin ELISA kit (80‐INSHU‐E01.1, ALPCO), Branched Chain Amino Acid Assay kit (ab83374, ABCAM), and β‐Hydroxybutyrate Colorimetric Assay kit (700 190, Cayman Chemical), respectively. Evaluation

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8/12/2023Great BCAA Quantification Kit From Abcam Bruce WangWe previously pharmacologically identified a small metabolite that regulates energy expenditure in mice. In this study, we want to explore if genetic manipulation of this metabolite via gene knockout ... Read Review

Branched Chain Amino Acid Assay Kit / BCAA Assay Kit from Abcam

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Branched Chain Amino Acid Assay Kit / BCAA Assay Kit from Abcam

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