Description
ScienCell's GeneQuery™ Human Shear Stress and Mechanotransduction qPCR array kit (GQH-SSM) is designed to facilitate gene expression profiling of 88 key endothelial cell genes responsive to shear stress and mechanotransduction. GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+ and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis