Description
ScienCell's GeneQuery™ Human Basal Cell Carcinoma qPCR Array Kit (GQH-BCC) is designed to facilitate gene expression profiling of key genes involved in (i) basal cell carcinoma (BCC) biology and carcinogenesis; (ii) targeted BCC therapies; and (iii) hedgehog signaling pathway. 88 genes are selected in this kit based on database and literature research. GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis