Anti-AGO4 antibody - Argonaute 4 AS09 617 from Agrisera

Product Specs
ItemAnti-AGO4 antibody - Argonaute 4
CompanyAgrisera
Price EUR329.00Supplier Page View Company Product Page
Catalog NumberAS09 617
ApplicationsCoImmunoprecipitation, Immunocytochemistry, Immunoprecipitation, Western blot
FormatLyophilized
ReactivityArabidopsis thaliana, Hyacinthus orientalis
TargetAnti-AGO4
HostRabbit
ClonalityPolyclonal
ImmunogenKLH-conjugated synthetic peptide chosen from Arabidopsis thaliana AGO4 sequence UniProt: Q9ZVD5, TAIR: At2g27040
NCBI Gene AliasesOCP11, OVEREXPRESSOR OF CATIONIC PEROXIDASE 11, T20P8_9, T20P8.9, ARGONAUTE 4
Research AreaRNAi/siRNA/miRNA
PurityImmunogen affinity purified serum in PBS pH 7.4
StorageStore lyophilized/reconstituted at -20°C; Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur adhering to the cap or sides of the tube
Molecular Weight103 kDa
Quantity50 µg

Specifications/Features

1: 4000 (WB), 5 µg of antibody per 1 gram of a fresh tissue (IP), 1: 100 (ICC)

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Agrisera

Box 57

Vännäs

Sweden

Phone: +46 (0) 935 33033

Email: info@agrisera.com
orders@agrisera.com

Company Profile

Website: http://www.agrisera.com

(1) Dominance modifiers at the Arabidopsis self-incompatibility locus retain proto-miRNA features and act through non-canonical pathwaysPLoS GenetApril 20, 2026Rita A. Batista, Eléonore Durand, Monika Mörchen, Jacinthe Azevedo-Favory, Samson Simon, Manu Dubin, Vinod Kumar, Eléanore Lacoste, Corinne Cruaud, Christelle Blassiau, et al.of finely ground powder of frozen immature buds. The anti-AGO1 (AS09 527, Agrisera) and anti-AGO4 (AS09 617, Agrisera) antibodies were added at a 1/500 dilution for the incubation step and their immobilization

(2) RH3 enhances antiviral defense by facilitating small RNA loading into Argonaute 2 at endoplasmic reticulum-chloroplast membrane contact sitesNat CommunFebruary 25, 2025Juan Huang, Juan Du, Yan Liu, Lu Lu, Yanzhuo Xu, Jianfei Shi, Qing Liu, Qi Li, Yang Liu, Yaqiu Chen, et al.BE7003), α-HA (1:2000, Easybio, BE2061), α-AGO1 (1:2000, Agrisera, AS09527), α-AGO4 (1:2000, Agrisera, AS09617), α-GFP (1:2000, Easybio, BE2001), α-Toc34 (1:2000, PhytoAB, HY1264S), α-H3 (1:2000, Easybio, BE7004),

(3) Barley AGO4 proteins show overlapping functionality with distinct small RNA-binding properties in heterologous complementationPlant Cell RepMarch 13, 2024Fabio Miloro, András Kis, Zoltán Havelda, Ágnes Dalmadiphosphinothricin (10 mg/L), assessing the protein level of AtAGO4 using an anti-AtAGO4 antibody (Agrisera, AS09 617), and via PCR amplification of the region of T-DNA inserted approximately 170 bp before translational

(4) Accumulation dynamics of ARGONAUTE proteins during meiosis in ArabidopsisPlant ReproductionJune 1, 2022Cecilia Oliver, German Martinezrabbit anti-AGO1 (1:200 AS09 527), -AGO2 (1:100, AS13 2682), -AGO5 (1:100, AS10 671), -AGO4 (1:100, AS09 617), -AGO6 (1:50, AS10 672), -AGO9 (1:100, AS10 673), and -AGO10 (1:50, AS15 3071) antibodies from Agrisera.

(5) The Arabidopsis F-box protein FBW2 targets AGO1 for degradation to prevent spurious loading of illegitimate small RNACell ReportsApril 12, 2022Thibaut Hacquard et al.RRID:AB_2224930" Rabbit polyclonal anti-AGO2,Agrisera,Cat# AS13 2682 Rabbit polyclonal anti-AGO4,Agrisera,"Cat# AS09617, RRID:AB_10507623" Rabbit polyclonal anti-HYL1,Agrisera,"Cat# AS06 136, RRID:AB_2233541" Rabbit polyclonal

(6) Atypical molecular features of RNA silencing against the phloem-restricted polerovirus TuYVNucleic Acids ResearchNovember 8, 2021Marion Clavel, Esther Lechner, Marco Incarbone, Timothée Vincent, Valerie Cognat, Ekaterina Smirnova, Maxime Lecorbeiller, Véronique Brault, Véronique Ziegler-Graff, Pascal GenschikAgrisera) diluted 1:5000 (v/v). AGO4 protein was detected using the anti-AGO4 antibody (rabbit polyclonal, AS09 617; Agrisera) diluted 1:5000 (v/v). Myc-tagged proteins were detected using anti-myc antibody (mouse monoclonal;

(7) Dynamic distribution of ARGONAUTE1 (AGO1) and ARGONAUTE4 (AGO4) in Hyacinthus orientalis L. pollen grains and pollen tubes growing in vitroProtoplasmaMay 1, 2020Niedojadło K, Kupiecka M, Kołowerzo-Lubnau A, Lenartowski R, Niedojadło J, Bednarska-Kozakiewicz E.

(8) AGO-unbound cytosolic pool of mature miRNAs in plant cells reveals a novel regulatory step at AGO1 loadingNucleic Acids ResearchOctober 10, 2019Ágnes Dalmadi, Péter Gyula, Jeannette Bálint, György Szittya, Zoltán Haveldaand AS10 710) one hour or with anti-AGO1, anti-AGO2 or anti-AGO4 (Agrisera, AS09 527, AS13 2682, and AS09 617) 2.5 h at a dilution of 1:7500 in 1% milk powder (1× PBST). After washing in PBST, the membrane was

(9) The developmental regulator PKL is required to maintain correct DNA methylation patterns at RNA-directed DNA methylation lociGenome BiologyDecember 1, 2017Rong Yang et al.Commassie Blue staining. A standard western blotting protocol was used with anti-AGO4 antibody (Agrisera, AS09617) at 1:5000 dilutions. Additional files

(10) Biogenesis of phased siRNAs on membrane-bound polysomes in ArabidopsiseLifeDecember 12, 2016Shengben Li et al.anti-AGO1 or anti-AGO4 antibodies (AGO1: AgriSera Cat# AS09 527 RRID:AB_2224930; AGO4: AgriSera Cat# AS09 617 RRID:AB_ 10507623). To test the RNA dependence for AGO1’s membrane association, the clarified plant extract

(11) A One Precursor One siRNA Model for Pol IV-Dependent siRNA BiogenesisCellOctober 8, 2015Jixian Zhai et al.2014). AGO4-RIP was performed as previously described (Ji et al., 2011) with commercial AGO4 antibody (Agrisera #AS09 617). RNA isolated from RIP was used for library construction with Illumina TruSeq Small RNA Sample Preparation

(12) SUVR2 is involved in transcriptional gene silencing by associating with SNF2-related chromatin-remodeling proteins in ArabidopsisCell ResearchDecember 1, 2014Yong-Feng Han, Kun Dou, Ze-Yang Ma, Su-Wei Zhang, Huan-Wei Huang, Lin Li, Tao Cai, She Chen, Jian-Kang Zhu, Xin-Jian Heagarose (Sigma, A7470), anti-Flag M1 agarose (Sigma, A 4596), and anti-AGO4 conjugated agarose (Agrisera, AS09617). After the agarose-bound proteins were washed, the proteins were boiled and run on SDS-PAGE for western blotting

(13) DTF1 is a core component of RNA-directed DNA methylation and may assist in the recruitment of Pol IVProceedings of the National Academy of SciencesMay 14, 2013Heng Zhang et al.transferred to PVDF membrane and probed with anti-GST (Sigma-Aldrich; G7781) or anti-AGO4 (Agrisera; AS09 617) primary antibodies. Membranes were then washed with TBS-T, incubated with HRPconjugated secondary antibodies,

(14) The Arabidopsis RNA-directed DNA methylation argonautes functionally diverge based on their expression and interaction with target lociThe Plant cellFebruary 1, 2010Ericka R Havecker, Laura M Wallbridge, Thomas J Hardcastle, Maxwell S Bush, Krystyna A Kelly, Ruth M Dunn, Frank Schwach, John H Doonan, David C Baulcombe

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Anti-AGO4 : Argonaute 4

Anti-AGO4 antibody - Argonaute 4 from Agrisera

Description

Specifications/Features

Agrisera

Citations (14)

Reviews

Anti-AGO4 antibody - Argonaute 4 from Agrisera

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