Fig 2: A: Representative confocal photomicrographs in left panels show hepatocytes from control (Lap1fl/fl) and L-CKO mice labeled with anti-lamin A/C Abs and color confocal photomicrographs in right panels show an overlay of anti-lamin A/C Abs (red) and LipiDye (green) labeling. Note intranuclear lamin A/C and lipids in L-CKO hepatocytes. Scale bars: 10 μm. B: Percentages of nuclei with nuclear envelope invaginations in hepatocytes from control and L-CKO mice. Columns show mean percentage of nuclei with nuclear invaginations, black circles indicate data from separate experiments (75–411 nuclei counted for each experiment and genotype), and error bars show SEM. ∗P < 0.05 by Student’s t test. C: Representative orthogonal confocal sections showing XY, XZ, and YZ planes of a partial view of a L-CKO mouse hepatocyte nucleus labeled with anti-Sun2 Abs (red) and LipiDye (green). Arrow indicates a LD inside a nuclear envelope invagination. Scale bar: 5 μm. D: Representative 3D reconstruction of a hepatocyte nucleus from a L-CKO mouse, based on confocal microscopy data from labeling with anti-Sun2 Abs (red) and LipiDye (green). Arrow indicates a LD inside a nuclear envelope invagination. Scale bar: 10 μm. LD, lipid droplet; L-CKO mice, Alb-Cre;Lap1fl/fl mice.