Fig 2: Western blot analysis of protein expression levels of key enzymes and transporters in glutamatergic and monoaminergic pathways in the EC of mice after CRS exposure. β-actin and GAPDH were used to normalize the expression levels of proteins in control group and CRS group. (A) The protein expression levels of SERT, TH, AADC, MAOA and VMAT2 in monoaminergic pathway were examined by western blott (* P<0.05, n=6-7 mice per group). (B) The protein expression levels of GAD1, EAATT2, GLUL and SNAT1 in glutamatergic pathway were analyzed in both groups. (* P<0.05; n=6-7 mice per group).

Fig 3: Effects of cPS1P on the expression of dopamine-related proteins in the MPTP and NSE-hαSyn PD mouse models. (A,B) Expression of TH in the substantia nigra pars compacta (SNpc) and striatum of MPTP and NSE-hαSyn PD mouse models, respectively (n = 5 mice/group/model). (C,D) Expression of VMAT2 and DAT in the SNpc and striatum of MPTP and NSE-hαSyn PD mouse models, respectively (n = 5 mice/group/model). The values indicate the mean ± SEM. The significant differences have been given in the graphs. * Significant difference between the control and model group; # Significant difference between the model and cPS1P co-treated mouse. p < 0.05 was considered significant difference between the groups, as measured with one-way ANOVA followed by Tukey’s multiple comparison test.

Fig 4: The protein levels of TH, SNAP25, VMAT2 and synataxin 1a in synaptosomes. Note: Effects of baicalin on the protein expression of TH, SNAP25, VMAT2 and Synataxin 1a in synaptosomes. Data are expressed as the mean ± SEM, n = 10. #p < 0.05 and ##p < 0.01 versus WKY + saline group; *p < 0.05 and **p < 0.01 versus SHR + saline group

Fig 5: mRNA levels of TH, SNAP25, VMAT2, and syntaxin 1a in synaptosomms. Note: Effects of baicalin on the mRNA expression of TH, SNAP25, VMAT2 and synataxin 1a in synaptosomes. Data are expressed as the mean ± SEM, n = 10. #p < 0.05 and ##p < 0.01 versus WKY + saline group; *p < 0.05 and **p < 0.01 versus SHR + saline group