Fig 1: KLF10 increased when experiencing physical inhibition to proliferation or during the formation of 3D renal tubule model in vitro.A Datasets published on GEPIA were examined for KLF10 transcript expression between tumor tissue (T) and normal tissue (N). KICH, Kidney chromophobe; KIRC, Kidney renal clear cell carcinoma; KIRP, Kidney renal papillary cell carcinoma. B Representative immunofluorescence staining image of KLF10 and Fucci in 2D cultured renal tubular cells. C Representative immunoblot analysis of KLF10, CCNB1 and CCND1 in low-/middle-/high-density group. ACTIN served as the standard. D Representative fluorescence image of Fucci in low-/middle-/high-density group. Rate of proliferation was calculated via percentage of Fucci positive cells. E Representative immunoblot analysis of KLF10, CCNB1 and CCND1 in early/middle/late stages of tubular formation in 3D renal tubular model. F Relative mRNA levels of KLF10 in different stages of tubular formation in 3D renal tubular model were examined. G Representative fluorescence image of Fucci during different stages of tubular formation in 3D renal tubular model (early stage: day 1–2, middle stage: day 3–4, late stage: day 5–6). ns p ≥ 0.05, **p < 0.01, ***p < 0.001 vs control group at the same experimental conditions.