Fig 1: Mxra8, L-SIGN, and TIM-1 enhance CHIKV infection in 293T cells. 293T, HAP1, and VeroS cells were transfected with either TIM-1-GFP, Mxra8 and GFP, L-SIGN and GFP, or GFP alone. 24 h post-transfection, cells were inoculated with either mKate-expressing (A) CHIKV strain 181/c25, (B) recombinant vesicular stomatitis virus containing the Lassa virus glycoprotein (rVSV?G-LASV), or (C) rVSV containing the Ebola virus glycoprotein (rVSV?G-EBOV) for 1 h. 12 h following infection the cells were enumerated in a flow cytometer. Relative infection was calculated as the proportion of infected cells (mKate+) among transfected cells (GFP+) normalized to infection levels in a GFP-only control. Data are presented as the mean ± SEM from three independent experiments. Data were compared with a two-way ANOVA with multiple comparisons, comparing row effects (receptors produced) to the GFP only control: **, p < .01; ****, p < .0001.