Fig 1: Unaltered synaptic density in cultivated retina. (A) To label the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals, vGluT1 antibody was used (green). Whereas the PSD-95 antibody (red) stained the postsynaptic terminals. Cell nuclei were labeled with DAPI (blue). (B) vGluT1+ signal area was not altered in co-culture or conditioned medium groups compared to controls. (C) More PSD-95+ signal area was observed in the co-culture (p = 0.049) and conditioned medium group (p = 0.022) compared to controls. ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer. Scale bar: 20 µm, values are presented as mean ± SEM, n = 8/group, * p < 0.05.