Anti-ACT antibody - Actin (polyclonal) AS13 2640 from Agrisera

Product Specs
ItemAnti-ACT antibody - Actin (polyclonal)
CompanyAgrisera
Price $365.56Supplier Page View Company Product Page
Catalog NumberAS13 2640
ApplicationsExpansion microscopy, immunofluorescence, Western blot
FormatLyophilized
ReactivityActinidia, Agostis stoloniferacv. ‘Penncross’, Arabidopsis thaliana, Brassica napus, Cucumis sativus, Cyanthobasis fruticulosa, Cynara cardunculus, Fragaria x ananassa, Glycine max, Hordeum vulgare, Nicotiana tabacum, Odontarrhena lesbiaca, Petrosimonia nigdeensis, Phaseolus vulgaris, Phaeodactylum tricornutum, Phoenix dactylifera, Picrorhiza kurroa,Salsola grandis, Salsola tragus, Setaria italica, Solanum tuberosum, Triticum aestivum, Vigna unguiculata, Vitis vinifera, Zea mays
TargetAnti-ACT
HostRabbit
Immunogenca. 100 amino acids of recombinant actin conserved more than 80% in Arabidopsis thaliana: actin-1 P0CJ46 AT2G37620, actin-2 Q96292 AT3G18780, actin-3 P0CJ47 AT3G53750, actin-4 P53494 AT5G59370, actin-5 Q8RYC2 At2g42100, actin-7 P53492 At5g09810, actin-8 Q96293 AT1G49240, actin-11 P53496, AT3G12110,actin-12 P53497 AT3G46520
ClonalityPolyclonal
Research AreaCell Biology
NCBI Gene AliasesMYH9_2, FRIZZY AND KINKED SHOOTS 2, ACT2, F27J15_1, T6D20.1, FIZ1, FIZ2, actin-11, AAc1, ENL2, F27J15.1, actin-12, ENHANCER OF LRX1 2, actin 7, actin 4, FRIZZY AND KINKED SHOOTS, actin 2, actin 1, F2O15.3, F13M22.12, T6D20_1, LIGHT STRESS-REGULATED 2, LSR2, ACTIN, MYH9.2, F2O15_3, F13M22_12, actin 8, DER1, ARABIDOPSIS ACTIN 1, AtACT7, DEFORMED ROOT HAIRS 1
PuritySerum
StorageStore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
Molecular Weight41.6 : 45 kDa

Specifications/Features

1 : 2500 with standard ECL (WB), 1: 250 (IF)

Questions and Answers

Can this actin antibody be used for Western blotting in Arabidopsis thaliana studies?

Yes, the ACT antibody has been shown to work effectively in Western blotting applications, as evidenced by its use in various studies involving Arabidopsis thaliana, including those examining protein interactions and gene expression regulation.

What dilution should I use for this antibody in immunofluorescence applications?

For immunofluorescence applications, the recommended dilution for the ACT antibody is 1:250, which has been utilized in studies to visualize actin in plant tissues.

Is this antibody suitable for detecting actin in different plant species?

Yes, the ACT antibody is reactive with several plant species, including Arabidopsis, barley, corn, cucumber, potato, tobacco, and wheat, making it versatile for studies across various plant systems.

How has this antibody been utilized in recent research?

Recent studies have utilized the ACT antibody to investigate cellular processes such as programmed cell death and root cap turnover in Arabidopsis thaliana, highlighting its role in understanding plant development and stress responses.

Agrisera

Box 57

Vännäs

Sweden

Phone: +46 (0) 935 33033

Email: info@agrisera.com
orders@agrisera.com

Company Profile

Website: http://www.agrisera.com

(1) Simultaneous overexpression of three enzymes of chloroplast metabolism fails to improve CO2 assimilation or biomassJournal of Experimental BotanyJune 24, 2026Pauline Lemonnier, Shellie Wall, Hussein Gherli, Beatriz Moreno-Garcia, Chidi Afamefule, Tracy Lawson, Christine A Raines, Patricia E Lopez-Calcagno(Lefebvre et al., 2005), FBP aldolase (Simkin et al., 2015), AGPase small subunit, and Actin (AS111739 and AS132640, respectively from Agrisera, via Newmarket Scientific, UK). Proteins of interest were detected using

(2) Towards Understanding the Bioactive Potential of Two Intriguing Nepeta Species: Metabolomic and Phylogenetic PerspectivesPlants (Basel)June 11, 2026Uroš Gašić, Tijana Banjanac, Luka Petrović, Jovana Petrović, Ladislav Luc, Branislav Šiler, Danijela Mišić, Milica Milutinovićmembranes were incubated for 2 h in primary anti-ACT antibody (Anti rabbit Polyclonal lyophilized, AS13 2640; Agrisera Antibodies, Sweden) using the procedure described above. The obtained signal intensities for

(3) Hypoxic and Fe‐Responses are Regulated by the ERFVII Factors and the PCO Branch of the N‐Degron Pathway According to Iron AvailabilityPlant, Cell & EnvironmentJune 1, 2026Yuri Telara, Moez Maghrebi, Mikel Lavilla‐Puerta, Noemi La Monaca, Giulia Ambrogini, Alessio Sbrana, Sara Delucchi, Pierdomenico Perata, Gianpiero Vigani, Beatrice Giuntoliused for the immunodetection of RAP2.33xHA. Actin‐11 was detected with a polyclonal primary antibody (AS13 2640, Agrisera, 1:5000 dilution) coupled with a goat anti‐rabbit IgG HRP secondary antibody (Agrisera AS09 602

(4) Dual Cytoplasmic and Chloroplastic Mechanisms Fine‐Tune Chloroplast Division through ARC3 Protein StabilityAdvanced ScienceMay 26, 2026Yang Yuan, Denghao Xiang, Ruiying Li, Zhichuang Yue, Zhipeng Wang, Honghong HuUK, #ab9085). Actin was used as a loading control (anti‐Actin, 1:5,000; Agrisera, Shanghai, China, #AS13 2640). 4.11 Cell‐Free Protein Degradation Assay Total proteins were extracted from 4‐week‐old Arabidopsis

(5) Assembly of two functionally-distinct protein import complexes in the outer membrane of plant chloroplastsNature CommunicationsApril 20, 2026Sreedhar Nellaepalli, Domagoj Baretić, Astrid F. Brandner, Sybille Kubis-Waller, Duorong Xu, Ziad Soufi, Shuyang Cheng, Sireesha Kodru, Jun Fang, Úrsula Flores-Pérez, et al.(1:100000)71; anti-atTic56 (1:5000)39, anti-atTic100 (1:5000)39, anti-atTic214 (1:5000)39, anti-actin (1:3000; AS132640, Agrisera); anti-histone H3 (1:1000; ab1791, Abcam); and anti-HA (1:1000; H6908, Sigma). The secondary

(6) AUXIN RESPONSE FACTOR thermostabilityNature communicationsMarch 27, 2026Wilkinson EG, Sageman-Furnas K, Pereyra ME, Borniego MB, Casal JJ, Strader LC.was removed, and imaged for loading control analysis, or with 1:5000 anti-Actin Antibody (Agrisera, AS13 2640). The membrane was blocked in 8% milk (Great Value) in TBS-T buffer for 1 hour. 1:5000 Anti-GFP (Agrisera,

(7) Gallic Acid‐Responsive microRNAs Reprogram Lignification During Drought Acclimation Process in SpearmintPlant Biotechnology JournalMarch 14, 2026Alessia D'Agostino, Gabriele Di Marco, Gerardo Pepe, Adelaide Teofani, Chiara Pontecorvi, Manuela Helmer‐Citterich, Antonella Canini, Angelo GismondiSchuell). Blots were incubated with the following primary antibodies: rabbit polyclonal anti‐β‐ACTIN (AS132640; Agrisera AB, Sweden) and rabbit polyclonal anti‐LACCASE (HPA040150; Merck, Milan, Italy). Finally,

(8) Biochemical and structural differences between C3 cotyledons and C4 leaves in species of Salsoloideae (Chenopodiaceae)Sci RepOctober 17, 2025Ferit Kocacinar, Faik Ceylan, Sabahattin Cömertpay(Santa Cruz Biotechnology, SC-2357, USA) secondary antibody for two hours at room temperature. Actin (Agrisera-AS132640, Sweden) antibody was used as the loading control. After that, membranes were treated with ECL substrate

(9) High Dose of Nickel Unbalances Carbon Metabolism and Nitrogen Assimilation in Barley (Hordeum vulgare L.)Plants (Basel)September 20, 2025Alessia De Lillo, Ivana De Rosa, Giorgia Capasso, Giorgia Santini, Concetta Di Napoli, Noemi Russo, Ermenegilda Vitale, Stefania Grillo, Sergio Esposito, Simone Landi(EC 4.1.1.31) (gentle gift of Prof. R.P. Walker, Sheffield, UK). Immunoblottings using anti-actin (AS13 2640) and anti-Tubulin β-chain (AS10 681) (Agrisera, Vännäs, Sweden) were used as reference for equal loading

(10) The nuclear pore complex acts as a hub for pri-miRNA transcription and processing in plantsNucleic Acids ResearchSeptember 17, 2025Lucia Gonzalo, Delfina Gagliardi, Camila Zlauvinen, Tomasz Gulanicz, Agustín L Arce, Josefina Fernandez, Damian A Cambiagno, Catharina Merchante, Agnieszka Zienkiewicz, Artur Jarmolowski, et al.appropriate primary (anti-GFP (Agrisera AS152987); anti-HYL1 (Agrisera AS09532); anti-ACTIN8 (Agrisera AS132640)) and secondary (polyclonal IgG conjugated with HRP (Agrisera AS09602)), antibodies to detect proteins

(11) VEL-dependent polymerization maintains the chromatin association of Polycomb proteins for the switch to epigenetic silencingMolecular CellSeptember 4, 2025Anna Schulten, Geng-Jen Jang, Alex Payne-Dwyer, Marc Fiedler, Mathias L. Nielsen, Eduardo Mateo-Bonmatí, Mariann Bienz, Mark C. Leake, Caroline DeanAgriSera) and anti-RFP/mScarlet (6G6, Chromotek) were diluted 1:1000, anti-Actin was diluted 1:5000 (AS132640, Agrisera). Secondary antibodies were HRP-coupled. Blots were washed with TBS containing 0.05% Tween-20

(12) Pectin Metabolism Influences Phloem Architecture and Flowering Time in Arabidopsis ThalianaAdvanced ScienceJuly 29, 2025Qing Zhang, Juan Du, Shuang Hao, Zhengmei Leng, Weiguo Liu, Chengjian Zhao, Yue Yu, Longquan Chen, Charles T. Anderson, Chaowen Xiaoantibody (1:5000, Agrisera, catalog No. AS152987), an anti‐Actin antibody (1:5000, Agrisera, catalog No. AS132640), and HRP conjugated goat anti‐rabbit IgG (1:10 000, Agrisera, catalog No. AS09602). The signal was

(13) A fungal effector promotes infection via stabilizing a negative regulatory factor of chloroplast immunityNat CommunJuly 29, 2025Kunqin Xiao, Feng Yang, Wenjing Cui, Anmo Li, Jeffrey A. Rollins, Jinxin Guo, Xinhua Sun, Fengting Wang, Xiaojie Wang, Xun Xu, et al.anti-MORF2 (1:1000, this work), rabbit anti-RbcL (AS03037, 1:10000; Agrisera), rabbit polyclonal anti-actin (AS132640, 1:5000; Agrisera), goat anti-mouse IgG-HRP (D110087, 1:5000; Sangon), or goat anti-rabbit IgG-HRP (A9169,

(14) Heterologous expression of Arabidopsis FLS2 in Nicotiana benthamiana confers responsiveness to Ralstonia flg22 and activation of anti‐bacterial immunityPlant Biotechnology JournalJuly 25, 2025Meng Li, Yujiao Chen, Yali Wei, Alberto P. Machoanti‐phosphorylated MAPK (Cell signalling, Dancers, USA, 4370S), anti‐actin (Agrisera, Vannas, Sweden, AS132640), anti‐GFP (Abicode, CA, USA, M0802‐3a) and anti‐HA (Roche, Basel, Switzerland, No.11583816001) antibodies. ROS

(15) In-depth amino acid mutational analysis of the key interspecific incompatibility factor Stigmatic Privacy 1Plant And Cell PhysiologyJuly 24, 2025Yoshinobu Kato, Shun Tadokoro, Shota Ishida, Maki Niidome, Yuka Kimura, Seiji Takayama, Sota Fujiiand 0.05% (v/v) Tween-20). Polyclonal antibody against Actin and GFP were purchased from Agrisera (AS13 2640, Vännäs, Sweden) and MBL (598, Tokyo, Japan), respectively, and used at a dilution of 1:5000. Polyclonal

(16) A Blast‐Resistant NLR Gene Confers Drought Resistance by Competitively Interacting with an E3 Ligase to Protect Phenylalanine Ammonia‐Lyase in RiceAdvanced ScienceJuly 21, 2025Denghao Xiang, Haifu Tu, Yang Yuan, Yilong Yao, Wanwen Liao, Huaijun Wang, Yu Yan, Yao Wang, Yu Chen, Di Liu, et al.(ABclonal, AE003, 1:5000 dilution). Actin was used as a loading control (anti‐actin, 1:5000; Agrisera, #AS132640). Conflict of Interest The authors declare no conflict of interest. Author Contributions D.X., H.T.,

(17) Natural variation of AcEGY3 mediates chloroplastic ROS homeostasis to confer kiwifruit thermotoleranceNat CommunJuly 4, 2025Chengcheng Ling, Jun Yang, Jiale Xu, Wei Tang, Yunyan Liu, Yingzhen Wang, Pengwei Li, Yan He, Zhiyin Ouyang, Shuqi Chen, et al.membranes were probed with the different antibodies. Anti-Actin antibodies were purchased from Agrisera (#AS132640). Anti-GFP antibodies were purchased from Chengdu Zen-Bioscience Co., Ltd (#300943). Anti-HA (#ABT2040)

(18) Streamline Protocol for Arabidopsis Apoplastic Fluid Isolation Enables a Detailed Proteomic View of the Plant Extracellular SpacePlant DirectJuly 2, 2025Kuo-En Chen, Marilee Karinshak, Richard D. Vierstraat a 1:5000 dilution, including RBCL (BIOSS, bs6988R), cFBPase (Agrisera, AS04043), actin (Agrisera, AS132640), histone H3 (Agrisera, AS10710), catalase1 (PhytoAB, PHY3681S), RPS6 (PhytoAB, PHY7297S), and RPL13‐1

(19) Switchable client specificity in a dual functional chaperone coordinates light-harvesting complex biogenesisSci AdvJune 13, 2025Alex R. Siegel, Gerard Kroon, Changqi Zhao, Peng Wang, Peter E. Wright, Shu-Ou ShanCHLH (PHY1691; 1:1000) were purchased from PhytoAB (CA); those for LHCa1 (AS01005; 1:2000) and actin (AS132640; 1:1000) were purchased from Agrisera (Vännäs). Antibodies against cpSRP43 (1:1000) and cpSRP54 (1:1000)

(20) JA‐Mediated Regulation of Amino Acid Homeostasis Adjusts Metabolic Flux and Enhances Spider Mite Tolerance via the SlJAZ8‐SlWRKY57‐SlAVT6s Module in TomatoAdvanced ScienceJune 10, 2025Yingchen Hao, Xiaolong Wang, Langchen Guo, Lijun Xiang, Enxi Luo, Peng Cao, Penghui Liu, Yue Zhong, Chun Li, Jun Lai, et al.immunoblotted with rabbit anti‐GFP (Abcam, ab290, 1:5000 dilution) or rabbit anti‐actin (Agrisera, AS132640, 1:5000 dilution) as the primary antibody and anti‐HRP (ABclonal, AS014, 1:5000 dilution) as the secondary

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Anti-ACT : Actin (polyclonal)

Anti-ACT antibody - Actin (polyclonal) from Agrisera

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Specifications/Features

Questions and Answers

Agrisera

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Anti-ACT antibody - Actin (polyclonal) from Agrisera

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