Fig 1: Immunogenic monoclonal antibodies differ in their capacity to promote DC activation. Immature DCs were treated with media, LPS, Control mAb, mAb1, mAb2, or mAb3 for 48 hours. (a) Flow cytometric analysis of percentage of activated DCs as measured by CD80, CD83, or CD86 expression. Results are expressed as the percent of marker expression. Results from 3 independent experiments are shown, individual points representing the mean of individual donor responses. (b) Analysis of cytokine release by DCs after treatment with the indicated test antigens. Results are expressed as fold change over media only control. The response of eight cytokines was measured by a multiplex cytokine analysis. Bar Graphs represent the mean ± SEM fold-change in cytokine levels to media control. P values were generated by a one-way ANOVA followed by a paired T-test of log10 transformed concentrations of each test article versus media. A response was considered positive if the fold change (p < .05) was >2-fold above media control (***p < .0005, **p < .005, and *p < .05)