Fig 1: FUT2 stimulates autophagy through the nuclear p53-DRAM1 axis in A549 cells. (A) RNA-seq sequencing showing the genes associated with autophagy or apoptosis regulated by FUT2 in A549 cells. (B) Representative Western blot showing that the knockdown of FUT2 inhibited the expression of DRAM1 in A549 cells. (C) Representative Western blot showing the plasma and nuclear p53 protein levels in A549 cells. (D) Representative image of immunofluorescence staining showing the distribution of p53 in FUT2-knockdown A549 cells or control cells. (E) Representative Western blot showing the expressions of DRAM1 and LC3-II in FUT2-knockdown A549 cells or control cells in the presence or absence of siDRAM1. (F) Co-immunoprecipitation (Co-IP) assay showing an interaction between FUT2 and p53 or DRAM1 in A549 cells. (* p < 0.05, ** p < 0.01, and *** p < 0.001).