Fig 1: Expression of cell adhesion and epithelial markers in primary trophoblast cultures. (A) Expression of cell adhesion and trophoblast markers at the RNA level by culture days. Cultures (n = 7) were analyzed at the CTB and EVT phase for mRNA expression of ITGA1, ITGA5, ITGA6, TEAD4, EPCAM, and CGA. (B) Representative gating strategy from one donor (RC2), to select singlet cells by forward-sideward scatter that were viable (7-AADneg). The figure shows dot blots of the markers studied in CTB (upper two panels) and EVT after 6 days of differentiation (lower two panels). (C) Expression of cell adhesion and epithelial markers at the protein level by culture days. Cultures (n = 4) were analyzed by flow cytometry at the CTB and EVT phase for surface expression of integrins, CDH1, and three members of the ErbB family.