Fig 2: Flow cytometric analysis of ADMSCs. Expression of CD105, CD90, CD34 and CD45 was detected in ADMSCs isolated from bilateral inguinal fat pads of healthy Sprague Dawley rats. The cells at the third passage were incubated with FITC-labeled monoclonal antibodies against CD90 and CD45, or PE-labeled CD34 and CD105 for 30 min at 4°C and analyzed by flow cytometry to determine the surface marker expression of the ADMSCs. The control samples were not labeled with antibodies. The ADMSCs were positive for the mesenchymal stem cell markers CD90 and CD105 (A, green line; B, red line), and negative for the hematopoietic marker CD34 and CD45 (C, green line; D, red line). The blue line represents the control. ADMSCs, adipose-derived mesenchymal stem cells; FITC, fluorescein isothiocyanate; PE, phycoerythrin.

Fig 3: Identification of BMSCs. Morphological images (×100 magnification) of BMSCs cultured continuously for (A) 7 and (B) 14 days. BMSCs at 4 passages were positive for (C) oil red O staining (×200 magnification) and (D) ALP staining (×400 magnification) after 3 weeks of osteogenesis induction. (E) BMSCs surface markers expressed CD29, CD44 and CD90, and were negative for hematopoietic cell surface antigen markers CD34 and CD45.