BD Horizon™ BV421 Goat Anti-Rabbit IgG Polyclonal 0.1 mg RUO 565014 from BD Biosciences

Product Specs
ItemBD Horizon™ BV421 Goat Anti-Rabbit IgG Polyclonal 0.1 mg RUO
CompanyBD Biosciences
Price $230.00Supplier Page View Company Product Page
Catalog Number565014
Quantity0.1 mg
ApplicationsFlow cytometry, Immunofluorescence
Concentration0.2 mg/ml
Conjugate/TagBV421
ReactivityGoat
TargetIgG
IsotypeIg
ClonePolyclonal
ClonalityPolyclonal
Regulatory StatusRUO
FormatAqueous buffered solution containing BSA and ≤0.09% sodium azide

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BD Biosciences

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Milpitas, California 95035

United States

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Website: www.bdbiosciences.com

(1) Enhanced γ-globin reactivation and sickle cell correction through a repressor-to-activator motif switch in the HBG1/2 promotersMolecular Therapy Nucleic AcidsSeptember 8, 2026Anne Chalumeau, Panagiotis Antoniou, Maria Bou Dames, Pierre Martinucci, Elena Retana, Pragya Gupta, Mike Firth, Muralidhar Reddivari, Aathira Sarath Chandran, Jonathan S. Yen, et al.staining with a secondary antibody recognizing rabbit IgG (1/200 BV421-conjugated anti-rabbit IgG, 565014, BD Biosciences). Flow cytometry analysis of CD36, CD71, GPA, BAND3, and α4-Integrin erythroid surface

(2) Thymic cortical epithelial Psmb11-encoded β5t in mouse and humanInt ImmunolJune 9, 2026Mei-Ting Yang, Akihide Shimizu, Mami Matsuda-Lennikov, Jie Li, Meleca Gluckman, Alison Jacques, Shigeo Murata, Rhonda R. Roberts, Tessa W. Caceres, Sandra S. Garcia-Buntley, et al.temperature, followed by the staining with a BV421-conjugated goat anti-rabbit IgG (BD Biosciences, Cat. #565014) for 30 minutes at room temperature. Data were acquired on a BD FACS Celesta flow cytometer and analyzed

(3) MX1+ effector T cells hyperactivation at the maternal-fetal interface in unexplained recurrent pregnancy lossJournal of Translational MedicineFebruary 25, 2026Miaoxian Ou, Linying Yu, Yijie Gao, Lijun Huang, Weinan Deng, Jiahui Zhang, Haijiao Zou, Dunjin Chen, Lili Du, Shuang ZhangProteintech) and CLEC9A (222185, Zenbio), corresponding to the fluorescent secondary antibodies BV421 (565014, BD Biosciences) and APC (ab150115, Abcam), respectively. Direct antibodies used included CD3-FITC (300452,

(4) Deficiency of lysosomal TMEM175 in myeloid macrophages exerts anti-tumor immunity via inflammasome and cross-presentation pathwayNature CommunicationsFebruary 14, 2026Ziqi Zhang, Xue li, Tianqi Lu, Jingyun Yang, Xuejiao Han, Zhenfei Bi, Jingwen Luo, Aqu Alu, Weiqi Hong, Siyuan Chen, et al.(BioLegend, 123110), FITC anti-mouse F4/80 (BioLegend, 123108), BV421 anti-rabbit IgG (BD Biosciences, 565014), BV650 anti-mouse CD11b (BioLegend, 101239), FITC anti-mouse CD11b (BioLegend, 101206), PE-Cy7 anti-mouse

(5) Microglia replacement by peripheral delivery of CSF1R inhibitor-resistant hematopoietic cellsMolecular TherapyFebruary 4, 2026Sonia I. Lombroso, Emily I. Church, Ryan N. Anderson, Carleigh A. O’Brien, Yixuan Zhu, Asif A. Dar, Freddy S. Purnell, Eli M. Levitt, Brian Temsamrit, Fazeela Yaqoob, et al.catalog no. ab209064, 1:200) with goat anti-rabbit (BV421, polyclonal, BD Biosciences, catalog no. 565014, 1:500). The typical panel used to assess KSL status of HSPCs was as follows: propidium iodide (red,

(6) Advantages of Cell Proliferation and Immune Regulation in CD146+NESTIN+ HUMSCs: Insights from Single-Cell RNA SequencingStem CellsJune 24, 2025Peng Huang, Xiaofei Qin, Chuiqin Fan, Huifeng Zhong, Manna Wang, Fuyi Chen, Maochuan Liao, Nanpeng Zheng, Hongwu Wang, Bingchun Lin, et al.N5413), followed by a secondary antibody, Goat Anti-Rabbit conjugated with BV421 (BD Pharmingen, Cat# 565014). For CD146, cells were directly stained with a conjugated antibody, CD146 (mouse anti-human, Alexa

(7) Recognition of Staphylococcus aureus by the pattern recognition molecules langerin, mannan-binding lectin, and surfactant protein D: the influence of capsular polysaccharides and wall teichoic acidFront ImmunolJanuary 7, 2025Kirstine Mejlstrup Hymøller, Stig Hill Christiansen, Anders Grønnegaard Schlosser, Uffe B. Skov Sørensen, Jean C. Lee, Steffen Thielbacteria were subsequently incubated with 2.5 µg/mL BV421 polyclonal goat anti-rabbit IgG (cat. no. 565014, BD Bioscience) for 45 min at 4°C and washed twice in HBS+, 0.2% goat serum before flow cytometry analysis

(8) SKI Regulates Medullary Thymic Epithelial Cell Differentiation to Control Peripheral T Cell Responses in MiceThe Journal of ImmunologyJuly 1, 2024Honyin Chiu, Kristin N Weinstein, Sabine Spath, Alex Hu, Stephanie Varela, Kazushige Obata-Ninomiya, Steven F ZieglerCCL21,R&D Systems,IC457R-100UG Purified α-mouse DCLK1,Abcam,AB31704 BV421 Goat anti-rabbit IgG,BD,565014 Near-IR fixable Viability dye,Biolegend,423105

(9) Methamphetamine induces a low dopamine transporter expressing immunophenotype without altering total number of peripheral immune cellsBasic & Clinical Pharmacology & ToxicologyNovember 1, 2023Adithya Gopinath, Tabish Riaz, Emily Miller, Leah Phan, Aidan Smith, Ohee Syed, Stephen Franks, Luis R. Martinez, Habibeh KhoshboueiNIR,N/A,N/A,Biolegend,423105,FC,1:1000 TH,poly/chicken,AF647,Encor,CPCA-TH,FC,1:50 DAT,poly/rabbit,N/A,Sigma,AB5802,FC,1:200 Rabbit,poly/goat,BV421,BD,565014,FC,1:25 Antibodies used in this study.

(10) Evolutionarily conserved amino acids in MHC-II mediate bat influenza A virus entry into human cellsPLOS BiologyJuly 6, 2023Okikiola M. Olajide, Maria Kaukab Osman, Jonathan Robert, Susanne Kessler, Lina Kathrin Toews, Thiprampai Thamamongood, Jacques Neefjes, Antoni G. Wrobel, Martin Schwemmle, Kevin Ciminski, et al.step, cells were secondarily stained with BV421 goat anti-rabbit antibody (BD Biosciences, catalog no. 565014, 1:200) and APC goat anti-mouse antibody (BD Biosciences, catalog no. 550826, 1:200) for 30 min on ice.

(11) NETosis and thrombosis in vaccine-induced immune thrombotic thrombocytopeniaNature communicationsSeptember 5, 2022Leung HHL, Perdomo J, Ahmadi Z, Zheng SS, Rashid FN, Enjeti A, Ting SB, Chong JJH, Chong BH.histone H3 (1:50, ab5103), anti-MPO (1 μg/mL, PE, BD 341642) and goat anti-rabbit IgG (1:400, BV421, BD 565014). Monocytes and low density granulocytes were identified using anti-CD14 (1:50, V500, BD 561391) and

(12) DAT and TH expression marks human Parkinson's disease in peripheral immune cellsNPJ Parkinson's diseaseJune 7, 2022Gopinath A, Mackie P, Hashimi B, Buchanan AM, Smith AR, Bouchard R, Shaw G, Badov M, Saadatpour L, Gittis A, et al.mg/mL DAT,Polyclonal/Rabbit,N/A,Sigma,AB5802,FC,1:200,0.02 mg/mL Rabbit,Polyclonal/Goat,BV421,BD,565014,FC,1:40,0.005 mg/mL Rat,Polyclonal/Goat,APC,BD,551019,FC,1:40,0.005 mg/mL CD14,MfP9/Mouse,FITC,BD,347493,FC,1:50,0.0005

(13) Immature Vascular Smooth Muscle Cells in Healthy Murine Arteries and Atherosclerotic Plaques: Localization and ActivityInternational journal of molecular sciencesFebruary 3, 2022Balatskiy A, Ozhimalov I, Balatskaya M, Savina A, Filatova J, Kalinina N, Popov V, Tkachuk V.A-11058, 4 μg/mL for flow cytometry, 8 μg/mL otherwise), BV421 Goat Anti-Rabbit IgG (BD Biosciences, 565014, 2 μg/mL), DyLight405 AffiniPure Donkey Anti-Rabbit IgG (H+L) (Jackson Immunoresearch 711-475-152 1:100),

(14) Extracellular vesicle-based interorgan transport of mitochondria from energetically stressed adipocytesCell metabolismSeptember 7, 2021Crewe C, Funcke JB, Li S, Joffin N, Gliniak CM, Ghaben AL, An YA, Sadek HA, Gordillo R, Akgul Y, et al.594,ThermoFisher,Cat #: A-21207 RRID:AB_141637 BV421 Goat Anti-Rabbit IgG (Flow Cytometry),BD Biosciences,Cat #: 565014 RRID:AB_2716308 "FITC Rat Anti-Mouse Ig, κ Light Chain (Flow Cytometry)",BD Biosciences,Cat #: 550003

(15) Behavioural Fever Promotes an Inflammatory Reflex Circuit in EctothermsInternational Journal of Molecular SciencesAugust 17, 2021Nataly Sanhueza et al.(5 mg/mL) primary monoclonal antibody (mAb). Stained cells were detected with Brilliant Violet 421 (565014, BD Bioscience, Franklin Lakes, NJ, USA). To elucidate whether spleen cells expressed CD4-1+ and/or

(16) TNFα increases tyrosine hydroxylase expression in human monocytesNPJ Parkinson's diseaseJuly 20, 2021Gopinath A, Badov M, Francis M, Shaw G, Collins A, Miller DR, Hansen CA, Mackie P, Tansey MG, Dagra A, et al.TH (Millipore-Sigma, AB152, 1:100) followed by a species-specific secondary (anti-Rabbit BV421, BD, 565014). After resuspending the sample in a final volume of 250 µL PBS, 5 µL of Invitrogen CountBright Absolute

(17) Nuclei isolation of multiple brain cell types for omics interrogationNature protocolsMarch 1, 2021Nott A, Schlachetzki JCM, Fixsen BR, Glass CK.UV-laser is available or not, a secondary antibody Goat anti-rabbit IgG (H+L) BV421 (BD Biosciences; 565014) or Goat anti-rabbit IgG (H+L) APC/Cy7 (Invitrogen; A-21039), respectively, can be used (at step 59).

(18) Quantitative assessment of glioblastoma phenotypes in vitro establishes cell migration as a robust readout of Crk and CrkL activityJournal of Biological ChemistryFebruary 6, 2021Taeju Park, Neka Large, Tom CurranAPC-conjugated anti-mouse IgG (BD Biosciences, 550826) and BV421-conjugated anti-rabbit IgG (BD Biosciences, 565014). Cells were analyzed by flow cytometry using the On-chip Sort (On-chip Biotechnologies). Protein purification

(19) A Genome-Wide CRISPR-Cas9 Screen Reveals the Requirement of Host Cell Sulfation for Schmallenberg Virus InfectionJournal of VirologyAugust 17, 2020Thiprampai Thamamongood, Andrea Aebischer, Valentina Wagner, Max W. Chang, Roland Elling, Christopher Benner, Adolfo García-Sastre, Georg Kochs, Martin Beer, Martin Schwemmlenumber 550826; BD Pharmingen) at 1:200; for LACV, anti-rabbit IgG antibody coupled to BV421 (catalog number 565014; BD Pharmingen) at 1:200; for RVFV, anti-rat IgG(H⫹L) cross-absorbed antibody coupled to Alexa Fluor

(20) A novel approach to study markers of dopamine signaling in peripheral immune cellsJournal of immunological methodsJanuary 1, 2020Gopinath A, Doty A, Mackie PM, Hashimi B, Francis M, Saadatpour L, Saha K, Shaw G, Ramirez-Zamora A, Okun MS, et al.Sub.,2uL anti-Rat,Polyclonal/Goat,APC,BD,551019,Secondary,2.5uL anti-Rabbit,Polyclonal/Goat,BV421,BD,565014,Secondary,2.5uL CD16,3G8/Mouse,APC,Biolegend,302012,Mono. Sub.,2uL (mfg) Mouse Isotype control,"IgG2b,k/Mouse",FITC,Biolegend,402208,Isotype,2uL Rat

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BD Horizon™ BV421 Goat Anti-Rabbit IgG Polyclonal 0.1 mg RUO

BD Horizon™ BV421 Goat Anti-Rabbit IgG Polyclonal 0.1 mg RUO from BD Biosciences

BD Biosciences

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BD Horizon™ BV421 Goat Anti-Rabbit IgG Polyclonal 0.1 mg RUO from BD Biosciences

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