Fig 1: Effect of insulin therapy on InsB:12-20-reactive Helios- and/or Neuropilin-1 (Nrp-1)-expressing Foxp3+ Tregs. Frequencies of InsB:12-20-reactive cells within the CD4+FoxP3+Nrp1+ T cell population (A, D), CD4+FoxP3+Helios+ T cell population (B, E), and CD4+FoxP3+Helios+Nrp1+ T cell population (C, F) are shown at 15 weeks of age in PLN and pancreas of NOD mice. Female NOD mice received four weekly subcutaneous injections, from 10 until 13 weeks of age, of InsB:8-24 peptide formulated in alum (Ins+alum), InsB:8-24 peptide dissolved in buffer (Ins), alum alone (alum), or left untreated (Untr). Data presented as median with interquartile range; symbols (n = 2-6) represent individual mice. *P = 0.05; Treg, regulatory T cell; NOD, non-obese diabetic; PLN, pancreas-draining lymph nodes.