Fig 2: The nuclear import of RPRM induced by ionizing radiation is mediated by IPO11(A) IPO11 deletion (?IPO11) blocks the nuclear import of RPRM in H460 cells with RPRM overexpression after exposed to 10 Gy X-irradiation.(B) The nuclear import of RPRM after radiation exposure is restored in IPO11 knockout cells re-expressing WT IPO11 (IPO11), but not in cells expressing N-terminal mutant IPO11 (?N-IPO11). Reprinted with permission from: Zhang et al., iScience, 2022, “RPRM negatively regulates ATM levels through its nuclear translocation on irradiation mediated by CDK4/6 and IPO11”, iScience, 25, 105115.

Fig 3: IPO11 is essential for the nuclear translocation of RPRM upon irradiation(A) An interaction between RPRM and IPO11 in H460-RPRM cells was confirmed by Co-IP performed 30 min after 10 Gy X-irradiation.(B) Representative immunofluorescence images of co-localization of RPRM (green) and IPO11 (red) in RPRM-/- MEFs re-expressing RPRM. Scale bar, 10 µm.(C) Change in the subcellular localization of RPRM in negative control H460-RPRM cells (?NC) and cells with IPO11 deletion (?IPO11) after 10 Gy X-irradiation. Scale bars, 10 µm.(D) Immunoblotting on the nuclear and cytoplasmic RPRM of H460-RPRM-?NC/?IPO11 cells 30 min after 10 Gy X-irradiation.(E and F) Changes in the nuclear and cytoplasmic RPRM in IPO11-deleted H460-RPRM cells re-expressing WT IPO11 (E) and dominant-negative IPO11 mutant (F) 30 min after 10 Gy X-irradiation.(G) Change of ATM protein levels in H460-RPRM-?NC/?IPO11 cells after 2 Gy X-irradiation.Data shown represent the means (±SEM) of three biological replicates, *p < 0.05, ***p < 0.001; significance was determined by unpaired two-sample t test.