Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091 from Abcam

Product Specs
ItemAlexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab199091
Quantity100 µL
ApplicationsFlow Cyt, Flow Cyt (Intra), ICC/IF, IHC-P
Concentration0.5 mg/ml
Conjugate/TagAlexa Fluor® 488
IsotypeIgG
CloneEPR25A
HostRabbit
ClonalityMonoclonal
Antibody TypeIsotype Control Antibody
ReactivityHuman

Questions and Answers

Can this isotype control antibody be used in flow cytometry experiments?

Yes, the Alexa Fluor® 488 Rabbit IgG isotype control has been utilized in flow cytometry experiments, as demonstrated in studies where it was used alongside specific antibodies to assess nonspecific labeling in various cell types, including HepG2 cells and others.

Is there any evidence of this antibody being used in immunohistochemical staining?

Yes, the Alexa Fluor® 488 Rabbit IgG isotype control has been referenced in immunohistochemical staining protocols. For instance, it was used as a secondary antibody in studies involving the staining of various proteins in tissue sections, indicating its applicability in this technique.

What role does this isotype control play in experimental setups involving cancer research?

The isotype control is crucial in cancer research experiments, particularly in validating the specificity of primary antibodies. For example, in studies involving oncolytic viruses and tumor imaging, the isotype control was used to ensure that observed staining was due to specific interactions rather than nonspecific binding.

How has this antibody been utilized in studies related to immune responses?

The Alexa Fluor® 488 Rabbit IgG isotype control has been employed in studies examining immune responses, such as in flow cytometry analyses of T cell populations. It serves as a control to distinguish between specific and nonspecific binding of antibodies targeting immune markers, thereby enhancing the reliability of the results.

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Characterisation of Bespoke Patient-Derived In Vitro Models of Ewing SarcomaCancers (Basel)February 4, 2026Elizabeth A. Roundhill, Elton J. R. Vasconcelos, John Davies, Susan A. BurchillControl Mouse IgG1, X0931 (Dako), Alexa Fluor 647 and 488 Rabbit IgG isotype controls (ab199093 and ab199091, Abcam Plc.)). Expression was detected for unconjugated primary antibodies using a goat anti-rabbit

(2) Proton beam therapy induces protective immunity via HMGB1-dependent signalingFrontiers in Public HealthJanuary 20, 2026Jialing Wen, Xuanzhang Tu, Wangcai Ren, Qiaojuan Wang, Yue Wang, Gang Guo, Kensuke Osada, Takashi Shimokawa, Akihisa Takahashi, Nakako Izumi Nakajima, et al.aliquots: one for anti-CRT (1:50, ab196158, Abcam, USA) staining and the other for isotype antibody (1:50, ab199091, Abcam, USA) staining (n = 3 samples per group). The antibody-treated cells were incubated on ice for

(3) Estrogen receptor α expression in tumor-infiltrating lymphocytes from patients with endometrial cancerOncol LettDecember 19, 2025Marcin A. Jedryka, Anna Slawek, Paulina Kubik, Daria Lorek, Anna Ewa Kedzierska, Andrzej Czekanski, Rafał Matkowski, Anna Chelmonska-Soytacat. no. AB194150; Abcam) or appropriate isotype control (AF488 rabbit IgG, clone: EPR25A; cat. no. AB199091; Abcam) at the same concentration as the specific antibody. The samples were washed twice with permeabilization

(4) AMPK is necessary for Treg functional adaptation to microenvironmental stress during malignancy and viral pneumoniaJournal of Clinical InvestigationMarch 18, 2025Manuel A. Torres Acosta, Jonathan K. Gurkan, Qianli Liu, Nurbek Mambetsariev, Carla Reyes Flores, Kathryn A. Helmin, Anthony M. Joudi, Luisa Morales-Nebreda, Kathleen Cheng, Hiam Abdala-Valencia, et al.1), anti-AMPKα1 (Abcam cat. no. ab32047), Alexa fluor 488-conjugated isotype control (Abcam cat. no. ab199091), or unconjugated isotype control (Abcam cat. no. ab172730). The following day, cells that were stained

(5) Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic stateCell reports. MedicineDecember 17, 2024Zhang X, Zhang C, Lu S, Dong J, Tang N, Wang Y, Han W, Pan X, Zhang X, Liu D, et al.flow cytometry antibodyAntibody (clone EPR3915),Abcam,Cat#ab195359; RRID: AB_2714026 isotype control,Abcam,Cat#ab199091; RRID: AB_2637027 GAPDH Antibody,Affinity Biosciences,Cat# AF7021; RRID: AB_2839421 GLUT1 Antibody,Affinity Biosciences

(6) Serum ADAM17 levels pre-antiviral therapy correlate with HIV patient immune reconstitutionHeliyonDecember 15, 2024Ying Lin, Ming Chen, Xuelian Zhu, xiaoya cui, Suyu Sun, Xingzhong Hu(BD, USA, catalogue number: 557746). The isotope was IgG-Alexa Fluor@488 (Abcam, UK, catalogue number: ab199091). All antibodies were directly labelled mouse-antihuman monoclonal antibodies. 2.4.2 Staining Blood

(7) Enhancer AAVs for targeting spinal motor neurons and descending motor pathways in rodents and macaquebioRxivAugust 3, 2024Emily Kussick, Nelson Johansen, Naz Taskin, Brooke Wynalda, Refugio Martinez, Erin L. Groce, Melissa Reding, Elizabeth Liang, Lyudmila Shulga, Cindy Huang, et al.control (BD Biosciences, 555749, 1:250 dilution), Alexa Fluor 488 rabbit IgG Isotype Control (Abcam ab199091, 1:250) or DAPI alone. Single-nucleus sorting was carried out on either a BD FACSAria II SORP or BD

(8) IRF1 governs the expression of SMARCC1 via the GCN5-SETD2 axis and actively engages in the advancement of osteoarthritisJournal of orthopaedic translationMarch 1, 2024Wang D, Zhang Y, Zhang L, He D, Zhao L, Miao Z, Cheng W, Zhu C, Zhu L, Zhang W, et al.Arg1 (ab96183, Abcam). Following this, the sections were incubated with the secondary antibody (1:500, ab199091, Abcam) at 37 °C for 15 min. Subsequently, the sections were incubated with horseradish-labeled streptavidin

(9) PIGT promotes cell growth, glycolysis, and metastasis in bladder cancer by modulating GLUT1 glycosylation and membrane traffickingJournal of Translational MedicineJanuary 2, 2024Mingyue Tan, Qi Pan, Chao Yu, Xinyu Zhai, Jianyi Gu, Le Tao, Dongliang XuAbcam). All readings were performed in triplicates and controlled using Alexa Fluor® 488 Rabbit IgG (ab199091; Abcam) or FITC Mouse IgG2a, κ Isotype Control RUO (555,573; BD Biosciences) to address nonspecific

(10) AMP-activated protein kinase is necessary for Treg cell functional adaptation to microenvironmental stressbioRxivDecember 1, 2023Manuel A. Torres Acosta, Nurbek Mambetsariev, Carla P Reyes Reyes Flores, Kathryn A. Helmin, Qianli Liu, Anthony M. Joudi, Luisa Morales-Nebreda, Jonathan Gurkan, Kathleen Cheng, Hiam Abdala-Valencia, et al.1), anti-AMPKα1 (Abcam cat. no. ab32047), Alexa fluor 488-conjugated isotype control (Abcam cat. no. ab199091), or unconjugated isotype control (Abcam cat. no. ab172730). The following day, cells that were stained

(11) Targeting FAcilitates Chromatin Transcription complex inhibits pleural mesothelioma and enhances immunotherapyJournal of Experimental & Clinical Cancer ResearchNovember 16, 2023Anand Singh, Nathanael Pruett, Shivani Dixit, Sudheer K. Gara, Haitao Wang, Roma Pahwa, David S. Schrump, Chuong D. Hoangantibody (Abcam, ab209959) or Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A]- Isotype control (Abcam, ab199091) according to manufacturer’s instructions. Data were acquired and analyzed on FACSCalibur™ flow cytometer

(12) Irf7 regulates the expression of Srg3 and ferroptosis axis aggravated sepsis-induced acute lung injuryCellular & molecular biology lettersNovember 9, 2023Ling X, Wei S, Ling D, Cao S, Chang R, Wang Q, Yuan Z.UK) primary antibody were added and incubated at 4℃ overnight. After that, secondary antibody (1:500, ab199091, Abcam) was added and incubated at 37 ℃ for 15 min, and then 40 μL horseradase-labeled streptomycin

(13) An Oncolytic Poxvirus Encoding hNIS, Shows Antitumor Efficacy and Allows Tumor Imaging in a Liver Cancer ModelMolecular cancer therapeuticsJuly 5, 2023Chaurasiya S, Valencia H, Zhang Z, Kim SI, Yang A, Lu J, Woo Y, Warner SG, Ede NJ, Fong Y.stained with AlexaFluor488-conjugated anti-calreticulin antibody (ab196158; Abcam) or isotype antibody (ab199091; Abcam). Cells were fixed with 4% paraformaldehyde and analyzed on a flow cytometer (Accuri C6; BD). Determination

(14) Engineering of a Bispecific Nanofitin with Immune Checkpoint Inhibitory Activity Conditioned by the Cross-Arm Binding to EGFR and PDL1BiomoleculesMarch 31, 2023Jacquot P, Muñoz-Garcia J, Fleury M, Cochonneau D, Gaussin R, Enouf E, Roze C, Ollivier E, Cinier M, Heymann D.control antibodies were used: recombinant Alexa Fluor 488 Rabbit IgG, monoclonal [EPR25A] (Abcam, #ab199091), and PE mouse IgG1 kappa isotype control antibody (Biolegend, #400111). The cell binding capacity of

(15) Spatially resolved gene regulatory and disease-related vulnerability map of the adult Macaque cortexNat CommunNovember 8, 2022Ying Lei, Mengnan Cheng, Zihao Li, Zhenkun Zhuang, Liang Wu, Yunong Sun, Lei Han, Zhihao Huang, Yuzhou Wang, Zifei Wang, et al.final dilution of 1:500) was added to experimental tube, rabbit IgG-monoclonal-isotype control (Abcam, ab199091, final dilution of 1:500) was added to control-isotype tube. After 30 min incubation at 4 °C, samples

(16) CDH18 is a fetal epicardial biomarker regulating differentiation towards vascular smooth muscle cellsNPJ Regenerative medicineFebruary 2, 2022Junghof J, Kogure Y, Yu T, Verdugo-Sivianes EM, Narita M, Lucena-Cacace A, Yoshida Y.conjugated antibodies used are as follows: WT1-Alexa488 (Abcam; ab202635), rb-IgG-Alexa-488 (Abcam; ab199091), CDH18-FITC (Biorbyt Ltd; orb7854), CD22-APC (BD; 562860) CD31-APC (BioLegend; 303116) and CD144-FITC

(17) Nanocell COVID-19 vaccine triggers a novel immune response pathway producing high-affinity antibodies which neutralize all variants of concernFrontiers in immunologyJanuary 1, 2022Gao SY, Amaro-Mugridge NB, Madrid-Weiss J, Petkovic N, Vanegas N, Visvanathan K, Williams BRG, MacDiarmid JA, Brahmbhatt H.Phosphate-Buffered Saline, 0.05% Tween® 20 (PBST) and incubated with HRP conjugated anti-rabbit (1:5000) (Cat. #ab199091, Abcam) or anti-mouse (1:5000) (Cat. #31430, ThermoFisher) IgG secondary antibody for 1 h at RT. The

(18) Targeted immunotherapy for HER2-low breast cancer with 17p lossScience translational medicineFebruary 10, 2021Li Y, Sun Y, Kulke M, Hechler T, Van der Jeught K, Dong T, He B, Miller KD, Radovich M, Schneider BP, et al.for IHC), anti-calreticulin antibody (ab196158; 1:100 diluted for flow cytometry), and isotype IgG (ab199091; 1:100 diluted for flow cytometry) were purchased from Abcam. Anti-IFNAR1 (clone MAR1–5A3; 10 μg/ml

(19) EZH2-Mediated microRNA-375 Upregulation Promotes Progression of Breast Cancer via the Inhibition of FOXO1 and the p53 Signaling PathwayFrontiers in geneticsJanuary 1, 2021Guan X, Shi A, Zou Y, Sun M, Zhan Y, Dong Y, Fan Z.Abcam Inc., Cambridge, MA, United States) at 4°C overnight and then with the secondary antibody (1:500, ab199091, Abcam) at 37°C for 15 min. Thereafter, the sections were incubated with 40 μl of horseradish peroxidase

(20) Protective role of Galectin‐7 for skin barrier impairment in atopic dermatitisClinical & Experimental AllergyAugust 1, 2020Takatsune Umayahara et al.FluorTM (AF) 488 conjugated rabbit anti–Gal‐7 mAb (ab207365; Abcam) or AF488‐conjugated rabbit IgG mAb (ab199091; Abcam). AF546‐conjugated phalloidin and AF647‐conjugated ZO‐1 were also stained (both from Thermo Fisher Scientific

Be the First to Write a Review!

Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control from Abcam

Questions and Answers

Abcam

Citations (28)

Reviews

Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map