Purified (azide-free) Rabbit Polyclonal Isotype Ctrl 910801 from BioLegend

Product Specs
ItemPurified (azide-free) Rabbit Polyclonal Isotype Ctrl
CompanyBioLegend
Price $108.00Supplier Page View Company Product Page
Catalog Number910801
Quantity100 µL
ApplicationsWB - Quality tested, IHC-P, ELISA - Verified
Concentration1mg/ml
Conjugate/TagUnconjugated
FormatPhosphate-buffered solution
ClonePoly29108
HostRabbit
ImmunogenThis antibody was derived from rabbits
ClonalityPolyclonal
IsotypeIgG
Antibody TypeIsotype Control

Questions and Answers

Can this isotype control antibody be used in flow cytometry applications?

Yes, the Purified Rabbit Polyclonal Isotype Control has been utilized in flow cytometry as a control for staining. For instance, in a study examining peripheral blood mononuclear cells, the isotype control was employed to ensure specificity in the detection of target antibodies ().

Is this antibody suitable for immunohistochemistry, and how has it been applied in research?

The isotype control antibody has been shown to work in immunohistochemistry applications. It was used as a negative control in a study analyzing neutrophils and neutrophil extracellular traps in human thrombemboli, helping to validate the specificity of the primary antibodies used in the staining process ().

What role does this isotype control play in studies involving macrophages?

The Purified Rabbit Polyclonal Isotype Control has been referenced in studies focusing on macrophage polarization and phagocytic activity. It serves as a control to assess the specificity of antibodies targeting CD206, which is crucial for understanding macrophage responses in various inflammatory conditions ().

How has this isotype control been utilized in the context of dengue virus research?

In research related to dengue virus infection, the isotype control was employed to ensure accurate interpretation of results when assessing the expression of TLR2 on monocytes. This application highlights its importance in validating findings related to immune responses during viral infections ().

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Company Profile

Website: biolegend.com

(1) Microfluidic nanomagnetically isolated neuron- and astrocyte-derived extracellular vesicles to differentiate Lewy body and Alzheimer's diseaseNPJ BiosensMarch 12, 2026Stephanie J. Yang, Andrew A. Lin, Hanfei Shen, Laura W. Pappalardo, Griffin B. Spychalski, Jean Rosario, Leah K. Forsberg, Kiera M. Grant, Joshua R. Buser, Rodolfo Savica, et al.isotype control antibodies at the same concentrations (rabbit IgG isotype control antibody, BioLegend, 910801 for GluR2 or mouse IgG2a isotype control antibody, Miltenyi Biotec, 130-106-546 for GLAST, biotinylated

(2) Activin A secretion by muscle-repairing macrophages induces heterotopic ossification in miceJ Clin InvestMarch 2, 2026Wenqiang Yin, Kazuo Okamoto, Asuka Terashima, Warunee Pluemsakunthai, Takehito Ono, Taku Ito-Kureha, Shizuo Akira, Yoshinobu Hashizume, Roland Baron, Satoshi Ueha, et al.with 200 ng/mL anti-GFP antibodies (catalog ab183734; abcam) and rabbit IgG isotype control (catalog 910801; BioLegend), followed by overnight incubation at 4°C. Sections were then treated with a biotinylated

(3) Targeting β-catenin degradation with GSK3β inhibitors induces cell death in acute lymphoblastic leukemiaNature CancerJanuary 8, 2026Kadriye Nehir Cosgun, Huda Jumaa, Mark E. Robinson, Zhangliang Cheng, Salim Oulghazi, Kohei Kume, David Fonseca Arce, Nikol Agadzhanian, Klaus M. Kistner, Etienne Leveille, et al.were incubated with antibodies recognizing MTA2 (8106, Abcam), CHD4 (72418, Abcam) or isotype control (910801, Biolegend) for 4 h at 4 °C. Precleared DNA fragments were incubated with the antibody-conjugated magnetic

(4) UBQLN2 is necessary for UBE3A-mediated proteasomal degradation of the domesticated retroelement PEG10Journal of Cell ScienceNovember 14, 2025Julia E. Roberts, Phuoc T. Huynh, Luis O. Carale, Alexandra M. WhiteleyUBQLN2 (6H9 clone, Novus Biologicals), M2 FLAG-tag (Sigma-Aldrich F3165), or IgG control (BioLegend 910801) were conjugated to protein A or G resin beads (50% slurry) at a ratio of 1:8, using 40 µl of resin

(5) Epigenetic silencing of interleukin-10 by host-derived oxidized phospholipids supports a lethal inflammatory response to infectionsImmunitySeptember 9, 2025Marco Di Gioia, Valentina Poli, Piao J. Tan, Roberto Spreafico, Anne Chu, Alex G. Cuenca, Zhongyang Wu, Mehdi Benamar, Laura Pandolfi, Federica Meloni, et al.phospho-EZH2 (S21) (Affinity Biosciences, Cat# AF3822) or rabbit IgG isotype control (BioLegend, Cat# 910801) followed by PE-conjugated anti-rabbit antibody (BioLegend, Cat# 406421) staining. Samples were acquired

(6) Targeting Melanoma Cell Adhesion Molecule as a Novel Therapeutic Approach for Acral MelanomaExperimental DermatologySeptember 2, 2025Yuka Tanaka, Takamichi Ito, Kiichiro Nishio, Keiko Tanegashima, Takeshi NakaharaMCAM (1:200, 81701; Cell Signalling Technology, Danvers, MA) and rabbit isotype control IgG (1:200, 910801; BioLegend, San Diego, CA). The secondary antibody was goat anti‐rabbit IgG conjugated with AlexaFluor488

(7) Brain-derived extracellular vesicle microRNAs in Lewy body and Alzheimer’s diseasebioRxivJune 12, 2025Stephanie J. Yang, Andrew A. Lin, Hanfei Shen, Laura W. Pappalardo, Griffin B. Spychalski, Jean Rosario, Leah K. Forsberg, Kiera M. Grant, Rodolfo Savica, Sid O’Bryant, et al.control antibodies at the same respective concentrations (rabbit IgG isotype control antibody, BioLegend, 910801 for GluR2; mouse IgG2a isotype control antibody, Miltenyi, 130–106-546 for GLAST) on 500 μL of pooled

(8) Flow Cytometric Analysis and Sorting of Murine Enteric Nervous System Cells: An Optimized ProtocolInt J Mol SciMay 18, 2025Faidra Karkala, Indy de Bosscher, Jonathan D. Windster, Savio Stroebel, Lars van Zanten, Maria M. Alves, Andrea Sacchetti(Dallas, TX, USA); sc-2025",1 µg/mL Control Rabbit IgG,-,Unconjugated,"Biolegend (San Diego, CA, USA); 910801",0.4 µg/mL Control Mouse IgG2a,,PE,BD Pharmingen™; 555574,1 µg/mL Secondary Antibody,Reactivity,,Supplier;

(9) Evaluating biomarkers in canine cytotoxic interface dermatitis reactions to account for clinical and histopathological similarities and differencesFrontiers in Veterinary ScienceJanuary 22, 2025Shriya Kannan, Neil B. Wong, Grace E. Ryan, Nia E. R. James, Ayodeji Ajayi, Janet E. Lubov, Clement N. David, Linda Wrijil, Nicholas A. Robinson, Kelly Hughes, et al.Biological), anti-canine IgG (catalog # 7316; Novus Biologicals) or isotype control (Biolegend catalog # 910801) at 1:100 dilution using a Dako automated slide staining machine. All sections were counterstained with

(10) Optical Methods for Determining the Phagocytic Activity Profile of CD206-Positive Macrophages Extracted from Bronchoalveolar Lavage by Specific Mannosylated Polymeric LigandsPolymers (Basel)December 30, 2024Igor D. Zlotnikov, Alexander A. Ezhov, Natalia I. Kolganova, Dmitry Yurievich Ovsyannikov, Natalya G. Belogurova, Elena V. Kudryashovaanti-CD206 antibodies (ab64693, Abcam, Cambridge, MA, USA, 1:100) or rabbit polyclonal control IgG (910801, Biolegend, California, USA) as a control for 2 h at 22 °C and subsequently with goat–anti-rabbit antibody

(11) Evidence of Neutrophils and Neutrophil Extracellular Traps in Human NMSC with Regard to Clinical Risk Factors, Ulceration and CD8+ T Cell InfiltrateInternational Journal of Molecular SciencesOctober 2, 2024Linda-Maria Hildegard Moeller, Carsten Weishaupt, Fiona SchedelAdditionally, an adjacent slice of every specimen was stained with isotype antibodies (rabbit IgG: Biolegend #910801, San Diego, CA, USA, 1:50 and mouse IgM: Biolegend #401601, San Diego, CA, USA, 1:50) as negative controls. Between

(12) RANKL inhibition reduces lesional cellularity and Gα(s) variant expression and enables osteogenic maturation in fibrous dysplasiaBone researchFebruary 20, 2024de Castro LF, Whitlock JM, Michel Z, Pan K, Taylor J, Szymczuk V, Boyce B, Martin D, Kram V, Galisteo R, et al.Rabbit and goat isotype control antibodies were used at similar concentrations (BioLegend, Cat No. 910801 and R&D Systems, Cat No. AB-108-C, respectively), and no significant nonspecific staining was observed

(13) Spatiotemporal analysis of SARS-CoV-2 infection reveals an expansive wave of monocyte-derived macrophages associated with vascular damage and virus clearance in hamster lungsMicrobiology spectrumJanuary 11, 2024Bagato O, Balkema-Buschmann A, Todt D, Weber S, Gömer A, Qu B, Miskey C, Ivics Z, Mettenleiter TC, Finke S, et al.#9661, 1:200), and rabbit anti-Uteroglobin (Abcam, #ab40873, 1:500). Isotype control rabbit (Biolegend, #910801), rat (Biolegend, # 400602), and mouse (Biolegend, #401402) antibodies were used. Secondary antibodies

(14) Cathepsin W, T-cell receptor-associated transmembrane adapter 1, lymphotactin and killer cell lectin like receptor K1 are sensitive and specific RNA biomarkers of canine epitheliotropic lymphomaFrontiers in Veterinary ScienceNovember 3, 2023Jadesola Temitope Olayinka, Akanksha Nagarkar, Diana Junyue Ma, Neil B. Wong, Andrew Romasco, Cesar Piedra-Mora, Linda Wrijil, Clement N. David, Heather L. Gardner, Nicholas A. Robinson, et al.rabbit-anti-canine/human CTSK/CTSO/CTSX/CTSO2 (catalog # 139662; US Biological) or isotype control (Biolegend catalog # 910801) at 1:100 dilution using a Dako automated slide staining machine. Briefly, antigen retrieval was performed

(15) Biomimetic System Based on Reconstituted Macrophage Membranes for Analyzing and Selection of Higher-Affinity Ligands Specific to Mannose Receptor to Develop the Macrophage-Focused MedicinesBiomedicinesOctober 12, 2023Igor D. Zlotnikov, Elena V. Kudryashovaanti-CD206 antibodies (ab64693, Abcam, Cambridge, MA, USA, 1:100) or rabbit polyclonal control IgG (910801, Biolegend, San Diego, CA, USA) as a control for 2 h at 22 °C and subsequently with goat–anti-rabbit

(16) Neutrophil S100A9 supports M2 macrophage niche formation in granulomasiScienceMarch 17, 2023Mizutani T, Ano T, Yoshioka Y, Mizuta S, Takemoto K, Ouchi Y, Morita D, Kitano S, Miyachi H, Tsuruyama T, et al.(2B10),BD Biosciences,Cat#565833; AB_2739373 Purified Rabbit Polyclonal Isotype Ctrl,BioLegend,Cat#910801; AB_2722735 HRP-conjugated goat anti-rabbit IgG,Jackson ImmunoResearch Lab,Cat#110-035-1442; AB_2307391 Purified

(17) Novel Potential Markers of Myofibroblast Differentiation Revealed by Single-Cell RNA Sequencing Analysis of Mesenchymal Stromal Cells in Profibrotic and Adipogenic ConditionsBiomedicinesMarch 10, 2023Olga Grigorieva, Nataliya Basalova, Maksim Vigovskiy, Mikhail Arbatskiy, Uliana Dyachkova, Maria Kulebyakina, Konstantin Kulebyakin, Pyotr Tyurin-Kuzmin, Natalia Kalinina, Anastasia EfimenkoVictoria, Australia, DF4245), RDH10 (Affinity Biosciences, DF12105), or rabbit polyclonal IgG (Biolegend, 910801) in 1% BSA solution at +4° overnight. Then, the samples were incubated with fluorescence-labeled goat

(18) Application Prospects of FTIR Spectroscopy and CLSM to Monitor the Drugs Interaction with Bacteria Cells Localized in Macrophages for Diagnosis and Treatment Control of Respiratory DiseasesDiagnostics (Basel, Switzerland)February 12, 2023Zlotnikov ID, Ezhov AA, Vigovskiy MA, Grigorieva OA, Dyachkova UD, Belogurova NG, Kudryashova EV.with a solution of anti-CD206 antibodies (ab64693, Abcam, 1:100) or rabbit polyclonal control IgG (910801, Biolegend) as a control for 2 h at RT and subsequently with goat-anti-rabbit antibody conjugated with

(19) Human epidermal growth factor receptor 3 serves as a novel therapeutic target for acral melanomaCell death discoveryFebruary 10, 2023Tanaka Y, Ito T, Kaku-Ito Y, Tanegashima K, Tsuji G, Kido-Nakahara M, Oda Y, Nakahara T.reported method [62]. After fixation and blocking, cells were incubated with isotype control (1:200, 910801; BioLegend, San Diego, CA) or rabbit anti-human HER3/ErbB3 (1:200) at 4 °C overnight. Cells were further

(20) Interleukin 36 receptor-inducible matrix metalloproteinase 13 mediates intestinal fibrosisFrontiers in immunologyJanuary 1, 2023Koop K, Enderle K, Hillmann M, Ruspeckhofer L, Vieth M, Sturm G, Trajanoski Z, Kühl AA, Atreya R, Leppkes M, et al.rabbit anti-human collagen type VI (Abcam, #ab182744), rabbit polyclonal isotype control (BioLegend, #910801), rat IgG2a isotype control (BioLegend, #400512). The following secondary antibodies were used: A555-labelled

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Purified (azide-free) Rabbit Polyclonal Isotype Ctrl from BioLegend

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Purified (azide-free) Rabbit Polyclonal Isotype Ctrl from BioLegend

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