Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 from Abcam

Product Specs
ItemRabbit IgG, monoclonal [EPR25A] - Isotype Control
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab172730
Quantity200 µg
ApplicationsChIC/CUT&RUN-seq, ChIP-seq, Flow Cyt, Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB
Conjugate/TagUnconjugated
IsotypeIgG
CloneEPR25A
HostRabbit
ClonalityMonoclonal
Antibody TypeIsotype Control Antibody

Questions and Answers

Can this isotype control antibody be used in RNA immunoprecipitation assays?

Yes, the Rabbit IgG, monoclonal [EPR25A] - Isotype Control has been utilized as an isotype control in RNA immunoprecipitation assays, as referenced in multiple studies, including those examining RNA interactions in various cellular contexts.

What applications has this antibody been shown to work in?

The Rabbit IgG, monoclonal [EPR25A] - Isotype Control has been shown to work in several applications, including Western blotting, flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry, immunoprecipitation, and chromatin immunoprecipitation sequencing.

Is there any specific concentration recommended for using this antibody in experiments?

In the literature, the recommended dilution for using the Rabbit IgG, monoclonal [EPR25A] - Isotype Control in various applications, such as chromatin immunoprecipitation, is typically around 1:100.

How has this isotype control antibody been referenced in recent scientific studies?

The Rabbit IgG, monoclonal [EPR25A] - Isotype Control has been cited in numerous scientific articles, including studies on chromatin remodeling and neuronal injury, indicating its relevance in various experimental contexts.

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(1) Lung cancer cell-derived exosomal EHF drives M2 macrophage polarization via transcriptional activation of RNF41 to promote tumor progressionRegenerative TherapyJune 1, 2026Zhongjie Chen, Junjie Ying, Gang Huang, Wenjun Shang, Ke Shi, Ze Wang, Li Wang, Dongxiao Ding, Chong Zhang, Yizheng Tanincubated with anti-EHF antibody (Catalog #: 27195-1-AP, Proteintech) or control anti-IgG (Catalog #: ab172730, Abcam) for immunoprecipitation. Following washes and cross-link reversal, precipitated DNA was extracted

(2) Poldip2 deficiency attenuates lung disease severity in a mouse model of COVID-19PLoS OneApril 29, 2026Ruinan Hu, Alejandra Valdivia, Taylor White, Willy Ju, Maegan L. Brockman, Zhan Zhang, Hongyan Qu, Georgette Gafford, Giji Joseph, Samantha Burton, et al.Sigma Aldrich) and CD31 (mouse, Ab9498, Abcam), or with the corresponding IgG isotype controls (ab18450 and ab172730, Abcam) were incubated overnight at 4°C. After washing with PBS, sections were incubated with anti-rabbit

(3) K63-linked ubiquitylation of S2P-RNAPII regulates transcription in a DNAPK inter-dependent manner in response to double-strand breaksNucleic Acids ResApril 28, 2026Vasiliki Pantazi, Paul Smith, Zoltan G Pahi, Manuela Katona, Adam Pap, Zsuzsanna Darula, Roman Fischer, Iolanda Vendrell, Benedikt M Kessler, Marcel A T M van Vugt, et al.USA) at 4°C overnight, followed by the addition of 40 μl of beads at 4°C for 4 h. Rabbit IgG (Abcam, ab172730, Cambridge, UK) and mouse IgG antibodies (Abcam, ab18443, Cambridge, UK) were used for the detection

(4) Vascular endothelial cell-derived small extracellular vesicles: A protective role against cardiomyocyte apoptosis in ischemia-reperfusion injury through miR410-3p-modulated smad7 pathwayJ Int Med ResApril 15, 2026Xiaoqiang Liu, Yulong Ge, Xing Xing, Junyi Liu, Hangwei Chen, Di Wang, Huaner Ni, Weifeng Li, Fang Wang(AGO2) antibody (Abcam, UK; 1:50; ab186733) or an immunoglobulin G (IgG) antibody (Abcam, UK; 1:50; ab172730) at 4°C. RNA was then purified and reverse-transcribed into cDNA for RT-PCR analysis. Statistical analysis All

(5) NSUN4-mediated m5C RNA methylation protects retinal cells against excitotoxic injury via the SHH signaling pathwayPLoS OneApril 15, 2026Yahong Li, Dian Li, Chao Geng, Ruihua Wei, Yajian Duanwith 5 μg of m5C antibody (ab214727, Abcam, Cambridge, UK) or negative control normal anti-rabbit IgG (ab172730, Abcam, Cambridge, UK) to capture the RNAs used for quantitative polymerase chain reaction (qPCR). Each

(6) Thrombospondin-1 triggers calreticulin expression in human mucoepidermoid carcinoma MC-3 cells via the PERK/CHOP pathwayOncol LettApril 14, 2026Shengwei Bao, Yuanyuan Zhu, Lijuan Guo, Sen Yangantibody (1:100; cat. no. ET1068-60; HUABIO) or an isotype-matched control antibody (1:100; cat. no. ab172730; Abcam) for 1 h at room temperature in the dark. After washing, the cells were incubated with a fluorescent

(7) Galectin-9high Neutrophils Exacerbate Radiation-Induced FrailtyAging CellApril 7, 2026Zhuo Cheng, Le Ma, Yan Chen, Jialun Li, Yali Dai, Wentao Xie, Yingjie Li, Tianlang Xu, Zhihe Hu, Weilin Huang, et al.R&D Systems #3535‐GA‐050), anti‐GAL‐9 antibody (1 mg, BioXCell#BE0218), or IgG isotype control (1 mg, Abcam #ab172730). C57BL/6J mice were used for local skin radiation, anesthetized with 4% isoflurane (RWD), and fixed

(8) Hsa_circ_0101645 contributes to excessive autophagy and apoptosis in intervertebral disc degeneration by acting as a miR-1304-5p sponge modulating BNIP3 expressionNon-coding RNA ResearchApril 1, 2026Jianhai Luo, Tong Yu, Pengcheng Hu, Jie LiuRNA Immunoprecipitation Kit (P0101; Geneseed, China), using AGO2 antibody (ab186733; Abcam, UK), and anti-IgG antibody (ab172730; Abcam). Finally, RT-qPCR was used to detect the expression of hsa_circ_0101645 in the immunoprecipitated

(9) Rapid expansion of primary human vocal fold epithelial cells via targeted pathway inhibition and anchorage-independent sphere cultureCell Rep MethodsMarch 23, 2026Xudong Shi, Ryo Suzuki, Haiyan Lu, Hua Zhang, Lingjun Li, Nathan V. Welham,Santa Cruz Biotechnology,Cat# sc-3878; RRID: AB_737242 "Rabbit monoclonal IgG, clone EPR25A (1:200 dilution)",Abcam,Cat# ab172730; RRID: AB_2687931 ,, Biological samples,Biological samples,Biological samples ,, Cadaveric human

(10) NRF2 Deficiency Disrupts Mitochondrial Homeostasis via NDUFS7 in Trabecular MeshworkResearch (Wash D C)March 20, 2026Xuejing Yan, Shen Wu, Xiaowei Fan, Qian Li, Yufei Teng, Ningli Wang, Jingxue Zhang10 μl of pre-cleared protein A/G agarose beads with anti-NRF2 (Abcam, ab62352) or anti-IgG (Abcam, ab172730) antibodies. The recovered DNA fragments were sequenced on the NovaSeq platform, and NRF2-binding sites

(11) The brain imaging feature-related gene NRP2 drives the malignant progression of glioblastoma through the FAK pathway: a Mendelian randomization studyTransl OncolMarch 19, 2026Yang Li, Jun Que, Yong Xia, Lei Wang, Pinshan Zhang, Zhen Cheng, Bo Gaoantibodies and was stored at −20 °C; the IgG group was added with rabbit anti-IgG antibody (Abcam, ab172730, UK); the IP group was added with the target antibodies Anti-FAK antibody(Proteintech, 12,636-1-AP,

(12) FBXO6 regulates colon cancer migration and invasion via ITGB1 ubiquitination and downstream signalingCell Death & DiseaseMarch 19, 2026Niansheng Ren, Long Cheng, Zijian Huang, Xuchen Hu, Fengxu Chi, Yuekun Zhu, Gang Wangwith RIPA buffer to extract proteins. For experimental controls [1]. IgG control: normal rabbit IgG (ab172730, Abcam) was used in place of primary antibody [2]. Blank bead control: protein A/G agarose beads without

(13) CASC19 stabilization by ALYREF via m5C modification and its impact on SCD in colorectal cancer cell aggressiveness and stemnessCell & BioscienceMarch 7, 2026Li Zhao, Rongjun Xie, Li Li, Xianzhou Lu, Li Wang, Hui Wang, Longfei LiuNo. ab19862), and m5C (Cat. No. ab10805) (Abcam), or an IgG isotype control (5 µg, Abcam, Cat. No. ab172730), were pre-incubated with magnetic beads at 4 °C for 2 h. The cell lysate was then added and incubated

(14) ACSL4 mediates ferroptosis to promote immunoglobulin A nephropathy progression: scRNA-seq analysisScientific ReportsMarch 7, 2026Ningjun Shao, Kuibi Tan, Ping Chen, Qun Luotemperature for 30 min and then washed three times with PBS for 5 min/time. The secondary antibody (ab172730, 1:100, goat anti-rabbit IgG, Abcam, UK) was added dropwise and incubated in the dark for 1 h. The sections

(15) TROP-2 Is Not a Therapeutic Target in Melanoma: a Morphological StudyDermatology and TherapyMarch 7, 2026Sonia Fantone, Daniela Marzioni, Elisa Molinelli, Alessandra Filosa, Gaia Goteri, Anna Campanati, Roberto Campagna, Giovanni Tossettacontrols were performed by omitting the first or secondary antibody or using an isotype control antibody (ab172730 and ab18443; Abcam, Cambridge, UK) at the same dilution of primary antibody. Sections were visualized

(16) Mechanism of N6-methyladenosine (m6A)-mediated upregulation of LINC00958 in the growth of cervical cancerFront OncolMarch 6, 2026Yuting Li, Zhengying Liu, Hongling JinHeLa cells (23) were re-suspended in RIP lysis buffer with anti-c-MYC (ab32072, Abcam) or anti-IgG (ab172730, Abcam). Then, the cells in each group were added with magnetic bead and proteinase K. Finally, the

(17) Interleukin-18 Inhibition Aggravates Atherosclerosis in Jak2V617F Clonal HematopoiesisJACC: Basic to Translational ScienceFebruary 25, 2026Mojdeh Tavallaie, Cheng-Chieh Hsu, Brian D. Hardaway, Huijuan Dou, Trevor Fidler, Eunyoung Kim, Sandra E. Schiavone, David Ngai, Tong Xiao, Nan Wang, et al.1:100), or rat isotype control IgG (BioLegend, 400431, 1:200) or rabbit isotype control IgG (Abcam, Ab172730, 1:150), and DAPI (BioLegend, 42280, 1:2,500). Sections were incubated with primary antibodies at 4

(18) Osteocytic Lipocalin-2 regulates bone formation locally through iron-dependent ferroptosis and Wnt suppressionCell Death DiscoveryFebruary 25, 2026Vivek Khanal, Madeline Carroll, Fatemeh Moradi, Jayden Carter, Ying Zhong, Chikkamagaluru G. Shashank, Amy Y. Sato, Ryan M. Allen, Umesh D. Wankhade, Neha S. DoleGoat Anti-Rabbit IgG H&L (1:1000, Abcam, ab150080, Alexa Fluor® 594). Corresponding nonimmune IgGs (Abcam, ab172730 for rabbit and R&D systems, AB-108-C for goat) were used as negative controls. H&E staining was performed

(19) S100A14 Facilitates Pancreatic Cancer Progression via S100A16-Mediated p53 SuppressionOncology ResearchFebruary 24, 2026Pingping Hu, Zhenhao Fei, Jianhua Bai, Zhiwen Wang, Yun Jin(ab233189, Abcam) and 2 μg anti-S100A16 antibody (ab240572, Abcam). In parallel, normal rabbit IgG (Abcam, ab172730) was used as an IgG isotype negative control (IP:IgG) to confirm the specificity of the immunoprecipitation.

(20) Collagen-mediated pro-tumorigenic MAPK activation drives stromal-immune reprogramming in solid cancersFrontiers in ImmunologyFebruary 24, 2026Junli Ding, Hongxin Lin, Hanfang Fan, Liangfeng Xu, Haihua Zhou, Huning Jiang, Zeyu Wang, Tiansong Xia, Yun Cai, Yichao Zhu, et al.(prediluted, Cat. GT2195, GeneTech), PMS2 (prediluted, Cat. GT2159, GeneTech), and IgG (1:100 dilution, Cat. ab172730, Abcam). Masson staining evaluation involved determining positively stained area percentages and IHC

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Rabbit IgG, monoclonal [EPR25A] - Isotype Control from Abcam

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Rabbit IgG, monoclonal [EPR25A] - Isotype Control from Abcam

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