Fig 1: Isolation of DDX4 Ab+ cells with different fluorescent probes. DDX4 Ab+ cells were detected using secondary Ab labeled with either AF594 (excited by 561 nm laser, upper panel) or AF647 (excited by 640 nm laser, lower panel). DDX4-AF594 detects 8.8% of the cells as DDX4 Ab+ and allows for better separation of the 2 populations compared to DDX4-AF647 that detects 5.2% DDX4 Ab+ cells. Both labeling strategies mainly target perivascular cells identified as positive for CD146 and CD9. Green marks cells positive for DDX4 Ab, and red marks cells double positive for CD146/CD9. As a negative control, the isotype control Ab recommended by Abcam (ab171870) was used. Abbreviations: Ab, antibody; FSC, forward scatter.