TNF-alpha Antibody

Product Specs
ItemTNF-alpha Antibody - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $469.00Supplier Page View Company Product Page
Catalog NumberNBP1-19532
ImmunogenA synthetic peptide made to an internal portion of the human TNF alpha protein (between residues 100-200) [Uniprot: P01375]
Concentration1.0 mg/ml
TargetTNF-alpha
FormatPBS
HostRabbit
ApplicationsIF/IHC,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunohistochemistry Free-Floating,Immunocytochemistry/ Immunofluorescence
Conjugate/TagUnconjugated
Quantity0.1 mg (also 0.025 mg)
ReactivityCanine,Fish,Human,Mouse,Primate - Macaca mulatta (Rhesus Macaque),Rat
ClonalityPolyclonal
PurityImmunogen affinity purified
Research AreaAdaptive Immunity, Asthma, Autoimmune Diseases, Autophagy, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
IsotypeIgG
Antibody TypePrimary Antibodies
NCBI Full Gene Nametumor necrosis factor
NCBI Gene AliasesTNFSF2, DIF, TNF-alpha, TNLG1F, TNFA
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Sizes Available: 0.1 mg (also 0.025 mg)

Questions and Answers

What species has the TNF-alpha antibody been shown to work with in research studies?

The TNF-alpha antibody has demonstrated reactivity with several species, including human, mouse, rat, canine, non-human primate, and zebrafish, making it versatile for various experimental models.

Can you provide examples of how this antibody has been utilized in scientific research?

The TNF-alpha antibody has been used in immunohistochemistry to assess inflammation and cytokine expression in various studies. For instance, it was employed to evaluate TNF-alpha levels in the context of oral mucositis in dogs, where it helped identify reductions in inflammatory cytokines following treatment with a Smad7-based biologic (). Additionally, it has been used to study TNF-alpha expression in models of diabetic retinopathy, highlighting its role in inflammatory responses in retinal cells ().

What applications is the TNF-alpha antibody suitable for?

The TNF-alpha antibody is suitable for multiple applications, including Western blotting, flow cytometry, immunocytochemistry, immunofluorescence, and immunohistochemistry. Its versatility allows researchers to utilize it in various experimental setups to study TNF-alpha's role in different biological processes.

How does the TNF-alpha antibody perform in terms of specificity and sensitivity in immunohistochemical applications?

The TNF-alpha antibody has been cited in numerous studies for its effectiveness in immunohistochemical applications, where it has been used to detect TNF-alpha expression in various tissues. For example, it was utilized in a study examining the inflammatory response in salivary glands, demonstrating its ability to accurately identify TNF-alpha levels in tissue sections ().

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Neuroprotective Effects of Polyphenol-Rich Corinthian Currant Against a Rotenone Parkinson's Disease Model: Mitigation of MAO-B and Pro-Inflammatory Cytokines Upregulation in Motor and Limbic Brain RegionsAntioxidants (Basel)July 22, 2026Eleni Fanarioti, Martha Tsarouchi, Christina Mountaki, Vaios Karathanos, Angeliki Chroni, Catherine R. DermonWaltham, MA, USA, Cat# P420B), rabbit anti-TNF-α (1:250, Novus Biologicals, Centennial, CO, USA, Cat# NBP1-19532), mouse anti-AChE (1:500, Santa-Cruz Biotechnology, Dallas, TX, USA, Cat# sc-373901) or mouse anti-MAO-B

(2) Intradiscal Cutibacterium acnes Sustains Modic Type 1-Like Lesions Over Time in a Rat Lumbar Endplate Injury ModelJOR SpineApril 22, 2026Irina Heggli, Alon Lai, Denise Iliff, Marco D. Burkhard, Niklas Koehne, Harsev Singh, Jonathan J. Huang, Alan C. Seifert, Damien Laudier, Noah Bonnheim, et al.the following primary antibodies: (i) rabbit polyclonal antibody against rat TNF‐α (1:500 dilution, #NBP1‐19532, Novus Biologics, MN, USA), (ii) rabbit recombinant multiclonal antibody against rat NE (1:500 dilution,

(3) Therapeutic potential of casticin in alleviating ovarian torsion/detorsion-induced ovarian and lung injuriesIran J Basic Med SciFebruary 9, 2025Ersen Eraslan, Ayhan Tanyeli̇, Mustafa Can Güler, Fazile Nur Eki̇nci̇ Akdemi̇r, Ömer Topdaği, Şahin Yazici, Derya Güzel Erdoğan, Esra Çinar Tanriverdi̇, Selim Çomaklispecific to 8-OHdG (Santa Cruz, Cat. No: sc-66036, Dilution: 1:250) and TNF-α (Novus Biological, Cat. No: NBP1-19532, Dilution: 1:100) were applied to the tissue sections, which were then incubated at room temperature

(4) Immuno-therapeutic and prophylactic potential of Trichinella spiralis antigens for inflammatory bowel diseasesFood and waterborne parasitologyDecember 1, 2024Wakid MH, El Kholy WA, Alsulami MN, El-Wakil ES.(MA5–16422, Thermo Fisher Scientific Co., Waltham, Massachusetts, USA) and Anti-TNF-α (1:100 dilution) (NBP1–19532, Novus Bio. Co., Easter Ave Centennial, USA) at 4 °C. The tissue sections were washed with PBS and incubated

(5) Immune response and bacterial resistance of Oreochromis niloticus against bacterial fish pathogen with saffron dietOpen Vet JOctober 31, 2024Heba A. Tolba, Ahmed M. Aldawek, Refaat A. Eid, Sherine Aladdin, Nahla H. El-Shaermethanol for 15 minutes, to block endogenous peroxidase activity, then the primary antibody (TNFα, NBP1-19532, dilution: 1/250) and (CD68, NB100-683, dilution: 1/1000) from Novus Biologicals (Littleton, CO, USA)

(6) Immune response and bacterial resistance of Oreochromis niloticus against bacterial fish pathogen with saffron dietOpen Veterinary JournalOctober 31, 2024Heba A. Tolba, Ahmed M. Aldawek, Refaat A. Eid, Sherine Aladdin, Nahla H. El-Shaermethanol for 15 minutes, to block endogenous peroxidase activity, then the primary antibody (TNFα, NBP1-19532, dilution: 1/250) and (CD68, NB100-683, dilution: 1/1000) from Novus Biologicals (Littleton, CO, USA)

(7) SARS-CoV-2 Spike Protein Exacerbates Thromboembolic Cerebrovascular Complications in Humanized ACE2 Mouse ModelTranslational Stroke ResearchOctober 2, 2024Stan P. Heath, Veronica C. Hermanns, Maha Coucha, Mohammed Abdelsaidmg/mL,1:1000,Rabbit Β-actin,R&D,MAB8929,1 mg/mL,1:1000,Mouse PAI-1,Biotechne,MAB-17861-100,1 mg/mL,1:1000,Rabbit TNFα,NOVUS,NBP1-19532,1 mg/mL,1:500,Rabbit TF-III,R&D,MAB-3178,1 mg/mL,1:1000,Mouse Antibodies

(8) Analgesic Effect of Human Placenta Hydrolysate on CFA-Induced Inflammatory Pain in MicePharmaceuticals (Basel)September 7, 2024Keun-Tae Park, Heejoon Jo, So-Hyun Jeon, Kyeongsoo Jeong, Minju Im, Jae-Won Kim, Jong-Pil Jung, Hoe Chang Jung, Jae hun Lee, Woojin KimUSA), anti-IL-1β (1:500 dilution, #NB600-633, Novus, Littleton, CO, USA), anti-TNF-α (1:500 dilution, #NBP1-19532, Novus, Littleton, CO, USA) and anti-actin (1:1000 dilution, #NB100-1617, Novus, Littleton, CO, USA).

(9) Retroductal dexamethasone administration promotes the recovery from obstructive and inflammatory salivary gland dysfunctionFrontiers in ImmunologyJuly 9, 2024Seungyeon Hwang, Jae-Min Cho, Yeo-Jun Yoon, Sunyoung Seo, Yongpyo Hong, Jae-Yol Limutilized with the ABC-HRP Kit (# PK-6200, VECTASTAIN, Newark, CA, USA). Primary antibodies against TNF (# NBP1–19532, 1:200; NOVUS, Centennial, CO, USA), IL-1β (# NB600–633,1:200; NOVUS), F4/80 (# A18637, 1:100; Abclonal,

(10) Direct-acting antivirals sofosbuvir and daclatasvir attenuate carbon tetrachloride-induced liver fibrosis in miceLiver researchMarch 1, 2023Abdelsalam MM, El-Mahdy N, Abou-Saif S.dilution 1/200), anti-MMP-9 (Cat.# PA5-13199, Thermofisher, USA; dilution 1/100) and anti-TNF-α (Cat.# NBP1-19532, Novus Bio. Co., USA; dilution 1/100). Afterwards, they were rinsed with phosphate-buffered saline (PBS)

(11) Lactate attenuates astrocytic inflammation by inhibiting ubiquitination and degradation of NDRG2 under oxygen-glucose deprivation conditionsJournal of neuroinflammationDecember 26, 2022Xu J, Ji T, Li G, Zhang H, Zheng Y, Li M, Ma J, Li Y, Chi G.antibody (1:5000; Sigma-Aldrich, HPA002896), rabbit anti-TNFα antibody (1:200, Novus, St. Louis, MO, USA; NBP1-19532), and rabbit anti-c-Jun (phospho S63) antibody (1:100; Abcam, ab32385), and the nuclei were counterstained

(12) Scutellarin attenuates hypertension-induced expression of brain Toll-like receptor 4/nuclear factor kappa BMediators of inflammationJanuary 1, 0001"Chen X, Shi X, Zhang X, Lei H, Long S, Su H, Pei Z, Huang R."

(13) Glycerol Improves Skin Lesion Development in the Imiquimod Mouse Model of Psoriasis: Experimental Confirmation of Anecdotal Reports from Patients with PsoriasisInternational journal of molecular sciencesJanuary 1, 0001"Choudhary V, Kaddour-Djebbar I, Custer VE, Uaratanawong R, Chen X, Cohen E, Yang R, Ajebo E, Hossack S, Bollag WB."

(14) Apoptotic vesicles restore liver macrophage homeostasis to counteract type 2 diabetesJournal of extracellular vesiclesJanuary 1, 0001"Zheng C, Sui B, Zhang X, Hu J, Chen J, Liu J, Wu D, Ye Q, Xiang L, Qiu X, et al."1:200), anti‐CD11b (101201, Biolegend, USA; diluted at 1:100), anti‐tumour necrosis factor‐alpha (TNF‐α) (NBP1‐19532, Novus Biologicals, USA; diluted at 1:100), anti‐CD206 (MCA2235, Bio‐Rad Laboratories, USA; diluted

(15) Aspirin Improves Nonalcoholic Fatty Liver Disease and Atherosclerosis through Regulation of the PPARδ-AMPK-PGC-1α Pathway in Dyslipidemic ConditionsBioMed research internationalJanuary 1, 0001"Han YM, Lee YJ, Jang YN, Kim HM, Seo HS, Jung TW, Jeong JH."

(16) P16^INK4a played a critical role in exacerbating acute tubular necrosis in acute kidney injuryAmerican journal of translational researchJanuary 1, 0001"Gu X, Peng CY, Lin SY, Qin ZY, Liang JL, Chen HJ, Hou CX, Wang R, Du YQ, Jin JL, et al."

(17) 5-HT(1A)R alleviates Aβ-induced cognitive decline and neuroinflammation through crosstalk with NF-κB pathway in miceInternational immunopharmacologyJanuary 1, 0001"Wang M, Zong HF, Chang KW, Han H, Yasir Rizvi M, Iffat Neha S, Li ZY, Yang WN, Qian YH."

(18) Siglec1 enhances inflammation through miR-1260-dependent degradation of IκBα in COPDExperimental and molecular pathologyJanuary 1, 0001"Li S, Jiang L, Yang Y, Cao J, Zhang Q, Zhang J, Wang R, Deng X, Li Y."

(19) Cardioprotective effect of caffeic acid phenethyl ester on cardiac contusion following blunt chest trauma in ratsBiotechnic & histochemistry : official publication of the Biological Stain CommissionJanuary 1, 0001"Bıçakçı N, Karaboğa I, Dökmeci AH, Güzel S, Fidanol Erboğa Z."

(20) "Loganin Ameliorates Painful Diabetic Neuropathy by Modulating Oxidative Stress, Inflammation and Insulin Sensitivity in Streptozotocin-Nicotinamide-Induced Diabetic Rats"CellsJanuary 1, 0001"Cheng YC, Chiu YM, Dai ZK, Wu BN."

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