Rabbit anti-PHD3 Antibody, Affinity Purified A300-327A from Bethyl Laboratories, Inc.

Product Specs
ItemRabbit anti-PHD3 Antibody, Affinity Purified
CompanyBethyl Laboratories, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberA300-327A
Quantity100 µg
ApplicationsWB
Concentration1000 µg/ml
Conjugate/TagUnconjugated
FormatWhole IgG
ReactivityHuman, Mouse
TargetPHD3
HostRabbit
Antibody TypePrimary
NCBI Full Gene Nameegl-9 family hypoxia inducible factor 3
NCBI Gene AliasesHIFPH3, PHD3, HIFP4H3
ClonalityPolyclonal
IsotypeWhole IgG
Domain/Region/Terminusbetween 50 and 100
Storage2 - 8°C

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Bethyl Laboratories, Inc.

P.O. Box 850

Montgomery, Texas 77356

United States

Phone: 1-800-338-9579
Fax: 1-866-597-6105

Email: orders@fortislife.com

Company Profile

Website: fortislife.com

(1) Homo-PROTACs: bivalent small-molecule dimerizers of the VHL E3 ubiquitin ligase to induce self-degradationNature CommunicationsDecember 1, 2017Chiara Maniaci, Scott J. Hughes, Andrea Testa, Wenzhang Chen, Douglas J. Lamont, Sonia Rocha, Dario R. Alessi, Roberto Romeo, Alessio Ciulli#3434) 1:1,000, anti-PHD2 (Bethyl Laboratories; A300-322A) 1:1,000, anti-PHD3 (Bethyl Laboratories; A300-327A) 1:1000, anti-CRBN (Proteintech; 11435-1-AP) 1:1,000. Following incubation with a horseradish peroxidase-conjugated

(2) Potent and selective chemical probe of hypoxic signalling downstream of HIF-α hydroxylation via VHL inhibitionNature communicationsNovember 4, 2016Frost J, Galdeano C, Soares P, Gadd MS, Grzes KM, Ellis L, Epemolu O, Shimamura S, Bantscheff M, Grandi P, et al.129395; 1:1000), anti-PHD2 (Bethyl Laboratories; A300-322A; 1:1,000), anti-PHD3 (Bethyl Laboratories; A300-327A; 1:1,000), anti-BNIP3 (abcam; ab10433; 1:10,000), anti-VHL (Cell Signaling Techonology; #2738; 1:1,000)

(3) Enhanced snoMEN Vectors Facilitate Establishment of GFP-HIF-1α Protein Replacement Human Cell LinesPloS oneJanuary 1, 2016Ono M, Yamada K, Bensaddek D, Afzal V, Biddlestone J, Ortmann B, Mudie S, Boivin V, Scott MS, Rocha S, et al.Cell Signaling), CAIX (NB100-417, Novus Biologicals), PHD2 (Bethyl A300-322A), BNIP3 (ab10433, Abcam), PHD3 (A300-327A, Bethyl labs) and Actin (3700, Cell Signaling) were used for immuno blotting analysis. Immunoprecipitation

(4) CDK-dependent phosphorylation of PHD1 on serine 130 alters its substrate preference in cellsJournal of cell scienceJanuary 1, 2016Ortmann B, Bensaddek D, Carvalhal S, Moser SC, Mudie S, Griffis ER, Swedlow JR, Lamond AI, Rocha S.(2867, Cell Signaling), 1:1000; BNIP3 (ab10433, Abcam), 1:2000; GLUT3 (53520, Anaspec), 1:1000; PHD3 (A300-327A, Bethyl labs), 1:1000; PHD1 (Bethyl A300-326A; Novus NBP1-40773), 1:1000; PHD2 (Bethyl A300-322A), 1:1000;

(5) Cezanne regulates E2F1-dependent HIF2α expressionJournal of cell scienceAugust 15, 2015Moniz S, Bandarra D, Biddlestone J, Campbell KJ, Komander D, Bremm A, Rocha S.(PA1-16510, Thermo Scientific), Cezanne (custom antibody, Eurogentec), β-actin (3700, Cell Signaling), PHD3 (A300-327A, Bethyl Labs), BNIP3 (ab10433, Abcam), Glut-1 (53519, Anaspec), p52 (05-361, Merck Millipore), E2F1

(6) Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxiaPloS oneJanuary 1, 2015Sun Y, George J, Rocha S.1:1000 dilution), anti-PHD2 (Bethyl #A300-322A, rabbit polyclonal, 1:1000 dilution), anti-PHD3 (Bethyl #A300-327A, rabbit polyclonal, 1:1000 dilution), anti-NDRG1 (Cell Signaling Technology #9485, rabbit monoclonal

(7) PHD1 links cell-cycle progression to oxygen sensing through hydroxylation of the centrosomal protein Cep192Developmental cellAugust 26, 2013Moser SC, Bensaddek D, Ortmann B, Maure JF, Mudie S, Blow JJ, Lamond AI, Swedlow JR, Rocha S.used: anti-PHD1 (Bethyl A300-326A; Novus NBP1-40773), anti-PHD2 (Bethyl A300-322A), anti-PHD3 (Bethyl A300-327A), anti-Cep192 1:200 (immunofluorescence) and 1:1,000 (western) (Bethyl A302-324A; Novus NBP-84634),

(8) The von Hippel-Lindau tumor suppressor protein regulates gene expression and tumor growth through histone demethylase JARID1COncogeneFebruary 9, 2012Niu X, Zhang T, Liao L, Zhou L, Lindner DJ, Zhou M, Rini B, Yan Q, Yang H.(Klose et al., 2007). Antibodies against PLU-1/JARID1B (A301-813A), JARID1C (A301-035A) and EGLN3 (A300-327A) were from Bethyl Laboratories (TX). Anti-GLUT1 antibody (NB300-666) and anti-HIF2α (NB100-132) were

(9) A super TLR agonist to improve efficacy of dendritic cell vaccine in induction of anti-HCV immunityPloS oneJanuary 1, 2012Bangxing Hong, Sung-Hyung Lee, Xiao-Tong Song, Lindsey Jones, Keigo Machida, Xue F Huang, Si-Yi Chen

(10) Autocrine stimulation of clear-cell renal carcinoma cell migration in hypoxia via HIF-independent suppression of thrombospondin-1Scientific reportsJanuary 1, 2012Bienes-Martínez R, Ordóñez A, Feijoo-Cuaresma M, Corral-Escariz M, Mateo G, Stenina O, Jiménez B, Calzada MJ.

(11) Mechanism of hypoxia-induced NF-kappaBMolecular and Cellular BiologyOctober 15, 2010Carolyn Culver, Anders Sundqvist, Sharon Mudie, Andrew Melvin, Dimitris Xirodimas, Sonia Rocha(sc-47701; Santa Cruz), anti-PHD1 (A300-62A-2; Bethyl Laboratories), anti-PHD2 (ab4561; Abcam), anti-PHD3 (A300-327A; Bethyl Laboratories), anti-Ubc13 (4919; Cell Signaling), anti-UbcH5a, anti-Senp1, anti-Senp2, anti-Senp6,

(12) The role of NF-kappaB in hypoxia-induced gene expressionAnnals of the New York Academy of SciencesOctober 1, 2009Cormac T Taylor, Eoin P Cummins

(13) Inhibition of prolyl hydroxylase domain proteins promotes therapeutic revascularizationCirculationJuly 7, 2009Loinard C, Ginouvès A, Vilar J, Cochain C, Zouggari Y, Recalde A, Duriez M, Lévy BI, Pouysségur J, Berra E, et al.

(14) Macrophage oxygen sensing modulates antigen presentation and phagocytic functions involving IFN-gamma production through the HIF-1 alpha transcription factorThe Journal of ImmunologyMarch 1, 2009Bárbara Acosta-Iborra, Ainara Elorza, Isabel M. Olazabal, Noa B. Martín-Cofreces, Silvia Martin-Puig, Marta Miró, María J. Calzada, Julián Aragonés, Francisco Sánchez-Madrid, Manuel O. Landázuri

(15) Prolyl hydroxylase PHD3 activates oxygen-dependent protein aggregationMolecular biology of the cellMay 1, 2008Rantanen K, Pursiheimo J, Högel H, Himanen V, Metzen E, Jaakkola PM.two independent specific primary antibodies at 1:2500 dilution: Rabbit polyclonal PHD3 (cat. no. BET-A300-327A, Bethyl Laboratories, Montgomery, TX) was used in Supplementary Figure 1 and Figure 6B and rabbit polyclonal

(16) Deficiency or inhibition of oxygen sensor Phd1 induces hypoxia tolerance by reprogramming basal metabolismNature geneticsFebruary 1, 2008Julián Aragonés, Martin Schneider, Katie Van Geyte, Peter Fraisl, Tom Dresselaers, Massimiliano Mazzone, Ruud Dirkx, Serena Zacchigna, Hélène Lemieux, Nam Ho Jeoung, Diether Lambrechts, Tammie Bishop, Peggy Lafuste, Antonio Diez-Juan, Sarah K Harten, Pieter Van Noten, Katrien De Bock, Carsten Willam, Marc Tjwa, Alexandra Grosfeld, Rachel Navet, Lieve Moons, Thierry Vandendriessche, Christophe Deroose, Bhathiya Wijeyekoon, Johan Nuyts, Benedicte Jordan, Robert Silasi-Mansat, Florea Lupu, Mieke Dewerchin, Chris Pugh, Phil Salmon, Luc Mortelmans, Bernard Gallez, Frans Gorus, Johan Buyse, Francis Sluse, Robert A Harris, Erich Gnaiger, Peter Hespel, Paul Van Hecke, Frans Schuit, Paul Van Veldhoven, Peter Ratcliffe, Myriam Baes, Patrick Maxwell, Peter Carmeliet

(17) Activation of HIF-prolyl hydroxylases by R59949, an inhibitor of the diacylglycerol kinaseThe Journal of biological chemistryJune 24, 2005Temes E, Martín-Puig S, Acosta-Iborra B, Castellanos MC, Feijoo-Cuaresma M, Olmos G, Aragonés J, Landazuri MO.

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Rabbit anti-PHD3 Antibody, Affinity Purified from Bethyl Laboratories, Inc.

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Bethyl Laboratories, Inc.

Citations (17)

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Rabbit anti-PHD3 Antibody, Affinity Purified from Bethyl Laboratories, Inc.

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