Flow Cytometry Fixation & Permeabilization Buffer Kit I FC009 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemFlow Cytometry Fixation & Permeabilization Buffer Kit I
CompanyR&D Systems, a Bio-Techne Brand
Price $249.00Supplier Page View Company Product Page
Catalog NumberFC009
Quantity1 Kit
ApplicationsFlow Cytometry
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Blocking Feedback Immunosuppression of Antigen Presentation in Brain Tumor During Oncolytic Virotherapy with oHSV-mshPKRMolecular Cancer TherapeuticsMarch 4, 2025Nobushige Tsuboi, Kimberly A. Rivera-Caraballo, Upasana Sahu, Rafal Pacholczyk, Eugene Douglass, Theodore S. Johnson, Qin Wang, Ravindra Kolhe, Catherine C. Hedrick, David H. Munn, et al.30 min as described(19). For intracellular staining, cells were permeabilized with Fix/Perm buffer (#FC009, R&D systems) for 20min and then washed with Perm/Wash buffer (R&D systems, Minneapolis, MN). All samples

(2) IKKε-deficient macrophages impede cardiac repair after myocardial infarction by enhancing the macrophage-myofibroblast transitionExperimental & molecular medicineSeptember 1, 2024Cho HH, Rhee S, Cho DI, Jun JH, Heo H, Cho SH, Kim D, Wang M, Kang BG, Yoo SJ, et al.intracellular molecules were detected using a Fixation and Permeabilization Buffer Kit (R&D Systems, FC009). The antibodies used are listed in Supplementary Table 4. Cell fluorescence was measured with a BD

(3) Rare presence and function of neuroendocrine cells in the nasal mucosaFrontiers in immunologyJanuary 1, 2024Wils T, Backaert W, Jacobs I, Ruysseveldt E, Cremer J, Dilissen E, Bullens DM, Talavera K, Steelant B, Van Gerven L, et al.intracellular staining was performed using Flow Cytometry Fixation & permeabilization Buffer Kit I (FC009, Biotechne) with 50 µl/ml PE Anti-Human Chromogranin A Clone S21-537 (RUO) antibody (5564563, BD Pharmingen).

(4) Hematopoietic and stromal DMP1-Cre labeled cells form a unique niche in the bone marrowSci RepDecember 16, 2023Sierra H. Root, Brya G. Matthews, Elena Torreggiani, Hector Leonardo Aguila, Ivo Kalajzicfixed and stained according to manufacture protocol using Flow Fix and Perm Buffer Kit (R&D Systems, FC009) and anti-Aire (Thermofisher, 14-5934-82) with a rabbit anti-rat FITC secondary. For histology, hydrated

(5) Discovery of a new marker to identify myeloid cells associated with metastatic breast tumoursCancer cell internationalNovember 18, 2023Bokil AA, Le Boulvais Børkja M, Wolowczyk C, Lamsal A, Prestvik WS, Nonstad U, Pettersen K, Andersen SB, Bofin AM, Bjørkøy G, et al.cells were washed twice with cold FACS buffer and fixed using the Fixation Buffer (R&D Systems, Cat #FC009) for 10 min at RT according to the manufacturer’s instructions. Following fixation, the cells were washed

(6) Vitamin B complex suppresses neuroinflammation in activated microglia: in vitro and in silico approach combined with dynamical modelingInternational immunopharmacologyAugust 1, 2023Rakić M, Lunić T, Bekić M, Tomić S, Mitić K, Graovac S, Božić B, Božić Nedeljković B.

(7) PKR induces TGF-β and limits oncolytic immune therapyJournal for ImmunoTherapy of CancerFebruary 16, 2023Bangxing Hong, Upasana Sahu, Matthew P Mullarkey, Evan Hong, Guangsheng Pei, Yuanqing Yan, Yoshihiro Otani, Yeshavanth Banasavadi-Siddegowda, Huihui Fan, Zhongming Zhao, et al.30 min as described.54 For intracellular staining, cells were permeabilized with Fix/Perm buffer (#FC009, R&D Systems, Minneapolis, Minnesota, USA) for 20 min and then washed with Perm/Wash buffer (R&D Systems).

(8) Ribonuclease 1 Enhances Antitumor Immunity against Breast Cancer by Boosting T cell ActivationInternational journal of biological sciencesJanuary 1, 2023Wang YN, Lee HH, Jiang Z, Chan LC, Hortobagyi GN, Yu D, Hung MC.min in the dark. After washing with PBS twice, the cells were fixed by fixation buffer (R&D Systems, #FC009) for 10 min at room temperature in the dark, washed twice with PBS, and resuspended the cell pellet

(9) Profiling of mature-stage human breast milk cells identifies six unique lactocyte subpopulationsScience advancesJuly 1, 2022Gleeson JP, Chaudhary N, Fein KC, Doerfler R, Hredzak-Showalter P, Whitehead KA.

(10) Oncolytic HSV therapy modulates vesicular trafficking inducing cisplatin sensitivity and anti-tumor immunityClinical Cancer ResearchJanuary 15, 2021Bangxing Hong et al.30 min as described (9). For intracellular staining, cells were permeabilized with Fix/Perm buffer (FC009, R&D systems) for 20min and then washed with Perm/Wash buffer (R&D systems, Minneapolis, MN). Fluorochrome

(11) Establishment of tumor inflammasome clusters with distinct immunogenomic landscape aids immunotherapyTheranosticsJanuary 1, 2021Liang Q, Wu J, Zhao X, Shen S, Zhu C, Liu T, Cui X, Chen L, Wei C, Cheng P, et al.intracellular staining was performed using Flow Cytometry Fixation & Permeabilization Buffer Kit I (#FC009, R&D Systems). IFN-γ (#554412, BD Pharmingen) and TNF-α (#554420, BD Pharmingen) antibodies were used

(12) DC-SIGN signalling induced by Trichinella spiralis products contributes to the tolerogenic signatures of human dendritic cellsScientific reportsNovember 20, 2020Cvetkovic J, Ilic N, Gruden-Movsesijan A, Tomic S, Mitic N, Pinelli E, Sofronic-Milosavljevic L.

(13) Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm StateCell stem cellNovember 5, 2020Zhang L, Avery J, Yin A, Singh AM, Cliff TS, Yin H, Dalton S.

(14) Human Umbilical Cord Blood Derived-Mesenchymal Stem Cells Alleviate Dextran Sulfate Sodium-Induced Colitis by Increasing Regulatory T Cells in MiceFrontiers in cell and developmental biologyJanuary 1, 2020Li Y, Ma K, Zhang L, Xu H, Zhang N.560408). Cells were fixed and permeabilized using fixation/permeabilization buffer kit (R&D Systems, FC009)for Foxp3 staining complying with the manufacturer’s instructions. The numbers of Treg gate represented

(15) Human Pluripotent Stem Cell-Derived Multipotent Vascular Progenitors of the Mesothelium Lineage Have Utility in Tissue Engineering and RepairCell reportsMarch 5, 2019Colunga T, Hayworth M, Kreß S, Reynolds DM, Chen L, Nazor KL, Baur J, Singh AM, Loring JF, Metzger M, et al.Intracellular markers were analyzed using Flow Cytometry Permeabilization/Wash Buffer I (1x, R&D Systems, FC009). Cell cycle analysis of MesoT cells was performed using a Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit

(16) Brucella canis induces canine CD4⁺ T cells multi-cytokine Th1/Th17 production via dendritic cell activationComparative immunology, microbiology and infectious diseasesFebruary 1, 2019Pujol M, Borie C, Montoya M, Ferreira A, Vernal R.

(17) Acute Drug Effects on the Human Placental Tissue: The Development of a Placental Murine Xenograft ModelReproductive sciences (Thousand Oaks, Calif.)December 1, 2018Verheecke M, Hermans E, Tuyaerts S, Souche E, Van Bree R, Verbist G, Everaert T, Cortès-Calabuig A, Van Houdt J, Van Calsteren K, et al.

(18) Trichinella spiralis Excretory-Secretory Products Induce Tolerogenic Properties in Human Dendritic Cells via Toll-Like Receptors 2 and 4Frontiers in immunologyJanuary 1, 2018Ilic N, Gruden-Movsesijan A, Cvetkovic J, Tomic S, Vucevic DB, Aranzamendi C, Colic M, Pinelli E, Sofronic-Milosavljevic L.

(19) Preconditioning of murine mesenchymal stem cells synergistically enhanced immunomodulation and osteogenesisStem cell research & therapyDecember 6, 2017Lin T, Pajarinen J, Nabeshima A, Lu L, Nathan K, Jämsen E, Yao Z, Goodman SB.

(20) MYC Controls Human Pluripotent Stem Cell Fate Decisions through Regulation of Metabolic FluxCell stem cellOctober 5, 2017Cliff TS, Wu T, Boward BR, Yin A, Yin H, Glushka JN, Prestegaard JH, Dalton S.× g for 4 minutes. Cells were resuspended in 0.5 mL of Flow Cytometry Fixation Buffer (R&D Systems, FC009) and incubated at room temperature for 10 min. Following fixation in 1% formaldehyde cells were washed

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Flow Cytometry Fixation & Permeabilization Buffer Kit I from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Flow Cytometry Fixation & Permeabilization Buffer Kit I from R&D Systems, a Bio-Techne Brand

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