PerCP/Cyanine5.5 anti-mouse CD3 100217 from BioLegend

Product Specs
ItemPerCP/Cyanine5.5 anti-mouse CD3
CompanyBioLegend
Price $118.00Supplier Page View Company Product Page
Catalog Number100217
NCBI Gene AliasesCtg-3, Ctg3, T3g
Clone17A2
ApplicationsFC - Quality tested
Conjugate/TagPerCP/Cyanine5.5
Quantity25 µg
ReactivityMouse
IsotypeRat IgG2b, kappa
HostRat
Antibody TypePrimary
NCBI Full Gene NameCD3 antigen, gamma polypeptide
Concentration0.2mg/ml
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenγδTCR-positive T-T hybridoma D1
ClonalityMonoclonal
TargetCD3

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Oncolytic virus OVV-03 enhances CAR-T cell therapy against glioblastoma via immune modulation and specific HER2 upregulationOncoImmunologyDecember 31, 2026Yi Tang, Qinzhi E, Liang Ma, Xuetao Li, Yang Zhang, Weichao Wang, Zuoyu Jiang, Xuewen Zhang, Yingbo Hou, Jiaming Du, et al.mouse splenocytes and tumor-infiltrating lymphocytes were stained with anti-CD3 (BioLegend, Cat.no. 100218), anti-CD45 (BioLegend, Cat.no. 103108), anti-CD4 (BioLegend, Cat.no. 100430), and anti-CD8 (BioLegend,

(2) Newly isolated human-derived Lactobacillus fermentum strain stimulates IFN-γ-secreting CD8+ T cells for enhanced anti-cancer immunityiScienceMay 15, 2026Tae-Young Kim, Seungil Kim, Sohyeon Lee, Su-Hyun Lee, Jiyoung Yu, Kyunggon Kim, Mi-Na Kweon,BD Biosciences,Cat# 557659; RRID: AB_396774 Anti-mouse CD3 Rat IgG monoclonal antibody,BioLegend,Cat# 100217; RRID: AB_1595597 Anti-mouse CD4 Rat IgG monoclonal antibody,Thermo Fisher Scientific,Cat# 25-0042-82;

(3) Type I interferon drives T cell responses to amyloid beta in the central nervous systemNat CommunApril 23, 2026Julius J. Michel, Khwab Sanghvi, Jakob Rosenbauer, Lea Humbs, Clara Tejido Dierssen, Saskia Grudzenski-Theis, Viktoria Sachs, Kristine Jähne, Karoline Degenhardt, Lutz Frölich, et al.at 37 °C. Migrated cells in the lower chamber were collected, stained for CD3(PercP Cy5.5, Biolegend 100218), CD8(PE-Cy7, Biolegend 100722), and CXCR3 (APC, Biolegend 126512), and analyzed by flow cytometry (ZE5 Cell analyzer

(4) A senescent tumor cell-derived nanovesicle directly primes splenic T cells to potentiate cancer radiotherapyCell Reports MedicineApril 21, 2026Jiaxing Pan, Shun Zhang, Yuchentian Xu, Zhuoliang Li, Meiqin Luo, Zirui Peng, Aojia Jin, Bo Hou, Xiji Qin, Xingang Cui, et al.17-0251-81; RRID: AB_469365 "Anti-mouse CD3 antibody (Clone 17A2), Percp-Cy5.5 conjugated",Biolegend,Cat# 100218; RRID: AB_1595492 "Anti-mouse CD4 antibody (Clone GK1.5), APC-Cy7 conjugated",BD Biosciences,Cat# 552051;

(5) Sunitinib induces immunogenic cell death through eIF2α phosphorylation to potentiate immunotherapy in HCCiScienceApril 17, 2026Yimei Gu, Hanbing Mai, Peijian Huang, Hui Yuan, Mingxuan Sun, Huixin Gao, Chenyu Shang, Yangfen Ou, Jizhou Tan, Xianzhang Huang, et al.Pacific Blue anti-mouse CD80,BioLegend,Cat#104723; RRID:AB_2076001 PerCP/Cyanine5.5 anti-mouse CD3,BioLegend,Cat#100218; RRID:AB_1595492 FITC anti-mouse CD8a,BioLegend,Cat# 100803; RRID:AB_312764 PE anti-mouse CD45,BioLegend,Cat#147711;

(6) TRIM36 and CAMK2N2 regulate ferroptosis and antigen presentation in small cell lung canceriScienceApril 17, 2026Yifan Cai, Shuang Zhu, Honglin Wang, Fang Huang, Zhongyuan Yin, Jun NieViability Kit (Zombie NIR Kit) (Biolegend: Cat#423105), CD45 (Biolegend: Cat#157607), CD3 (Biolegend: Cat#100217), IFNγ (Biolegend: Cat#505829) and CD8 (Biolegend: Cat#100751). Flow cytometry was used to gate the

(7) Extracellular vesicles from Akkermansia muciniphila protect streptozotocin-induced diabetic mice by regulating glucose homeostasis, oxidative stress, and immune toleranceFront ImmunolApril 7, 2026Yunxiang Chang, Cheng Zhang, Zhipeng Li, Shikai Wang, Jialin Wu, Jiacheng Xie, Xinsheng Chengfluorochrome-conjugated antibodies against CD3 (PerCP/Cyanine5.5, clone 17A2, BioLegend, catalog no. 100218), CD4 (FITC, clone RM4-5, BioLegend, catalog no. 100510), and CD25 (APC, clone PC61, BioLegend, catalog

(8) DNA delivered by lipid nanoparticles induces CD8+ T cell–dependent antitumor responses and enhances anti–PD-L1 therapyJCI InsightMarch 23, 2026Seoyun Yum, Alba Rodríguez-Garcia, Joan Castellsagué, Marta Giménez-Alejandre, Guillem Colell, Salut Colell, Teresa Lobo-Jarne, Mark A. LaRue, Michael A. Minnier, Mustafa N. Yazicioglu, et al.from BioLegend were used: CD16/32 for blocking (101301, clone 93), CD45.2 (103127, clone 30-F11), CD3 (100217, clone 17A2), CD4 (100527, clone RM4-5), CD8 (100707, clone 53-6.7), NK1.1 (108709, clone PK136), CD69

(9) A single-dose mRNA vaccine induces potent and long-lasting humoral and cellular immunity against the varicella-zoster virus in a murine modelFrontiers in ImmunologyMarch 23, 2026Wenyu Cheng, Ning Ren, Li Xie, Wenjin Zhou, Rui Quan, Heng Weianalysis of memory T cells, the cells were incubated with PerCP/Cyanine5.5 anti-mouse CD3 antibody (100218, Biolegend), FITC anti-mouse CD4 antibody (100204, Biolegend), PE anti-mouse CD62L antibody (161204,

(10) ATAD2 drives immunotherapy resistance by promoting lactic acid-mediated CD8+ T cell dysfunction in lung adenocarcinomaFrontiers in ImmunologyMarch 19, 2026Wanfeng Gao, Jialei Xu, Yue Li, Jingchang Zhang, Chenghao Ma, Junfeng Chen, Jiajing Chen(BioLegend, 423102), APC/Cyanine7 anti-CD45 (BioLegend, 103116), PerCP/Cyanine5.5 anti-CD3 (BioLegend, 100218), Brilliant Violet 605TM anti-CD8 (BioLegend, 100744), PE/Cyanine7 anti-NK1.1 (BioLegend, 156514), APC

(11) Weaning drives microbiome-mediated epigenetic regulation to shape immune memory in miceNature MicrobiologyMarch 19, 2026Li Yang, Robert C. Peery, Shirui Zhou, Xiaomin Chen, Leah M. Farmer, Fabiola Gutierrez, Stephanie Fowler, Lanjing Zhang, Julia M. Salamat, Karen Riggins, et al.103145, 1:100), TCRβ (BUV395, BD, clone H57-597, 569248, 1:100), CD3 (PerCP/Cy5.5, BioLegend, clone 17A2, 100217, 1:50), CD4 (APC/Cy7, BioLegend, clone RM4-5, 100525, 1:50), CD8a (Alexa Fluor 700, BioLegend, clone

(12) Fluorination augments tumor engagement and antitumor immunity of a D-peptide-based radiotheranostic agent for combinatorial immune checkpoint blockade therapyJ Immunother CancerMarch 9, 2026Siqi Zhang, Can Liu, Qingshuang Lu, Wenhao Liu, Jiang Wu, Jianjing Liu, Lina Tong, Kuo Zhao, Qichen Hu, Xueyao Chen, et al.antibodies were purchased from Thermo (Waltham, USA), and anti-CD11b-APC-CY7 (101226), anti-CD3-PerCP-cy5.5 (100218), anti-XCR1-BV421 (148216), anti-PD-L1-PerCP-cy5.5 (124334), anti-iNOS-PE (696805) were purchased from

(13) High-salt diet aggravates skin inflammation of psoriasis-like mouse model with CCL20‒CCR6 axis further activationJID InnovationsMarch 1, 2026Mengyan Hu, Han Cao, Li Zhang, Yuhan Xia, Jiayi Zhang, Feng Xue, Xia Li, Jie ZhengCD45.2 antibody (BioLegend, catalog number 109822), PerCP/Cy5.5 antimouse CD3 antibody (BioLegend, catalog number 100218), and allophycocyanin antimouse TCRγ/δ antibody (BioLegend, catalog number 118119) and then incubated

(14) Intein‐based modular chimeric antigen receptor platform for specific CD19 / CD20 co‐targetingMolecular OncologyMarch 1, 2026Pablo Gonzalez‐Garcia, Noelia Moares, Wenjie Yi‐He, Rosa Luna‐Espejo, Ricardo Fernandez‐Cisnal, Javier Ocaña‐Cuesta, Juan P. Muñoz‐Miranda, Antonio Gabucio, Cecilia M. Fernandez‐Ponce, Francisco Garcia‐Cozarwashed and labeled with anti‐human CD69‐APC (Cat# 310909, RRID:AB_314844) and CD3‐PerCP/Cy5.5 (Cat# 100218, RRID:AB_1595492) antibodies (both from Biolegend). To exclude dead cells and debris from analysis,

(15) Deficiency of lysosomal TMEM175 in myeloid macrophages exerts anti-tumor immunity via inflammasome and cross-presentation pathwayNature CommunicationsFebruary 14, 2026Ziqi Zhang, Xue li, Tianqi Lu, Jingyun Yang, Xuejiao Han, Zhenfei Bi, Jingwen Luo, Aqu Alu, Weiqi Hong, Siyuan Chen, et al.(BioLegend, 505829), PE-Cy7 anti-mouse-IFN-γ (BioLegend, 505826), PerCP-Cy5.5 anti-mouse CD3 (BioLegend, 100218), PE anti-mouse CD3 (BD Biosciences, 555275), BV421 anti-mouse CD3 (BioLegend, 100227), PE-Cy7 anti-mouse

(16) Immunotherapy targeting drug-tolerant Mycobacterium tuberculosis persisters accelerates tuberculosis cure in preclinical modelsJournal of Clinical InvestigationFebruary 3, 2026Styliani Karanika, Tianyin Wang, Addis Yilma, Jennie Ruelas Castillo, James T. Gordy, Hannah Bailey, Darla Quijada, Kaitlyn Fessler, Rokeya Tasneen, Elisa M. Rouse Salcido, et al.BV421-conjugated anti-CD45 (BioLegend catalog 103134), PerCPCy5.5 conjugated anti-CD3 (BioLegend, catalog 100218); PE/Dazzle 594–conjugated anti-CD4 (BioLegend, catalog 100566); Alexa Fluor 700–conjugated anti-CD8

(17) Single-immunocyte transcriptomics reveal the role of natural killer cell-dependent exogenous antigen presentation in ankylosing spondylitis severityExperimental & Molecular MedicineJanuary 28, 2026Dianshan Ke, Hanhao Dai, Yibin Su, Hongyi Zhu, Xiaofeng Liu, Xiaochun Bai, Changqing Zhang, Jie Xu, Jinshan Zhang(346708, BioLegend), HLA-DRB1-PE (362301, BioLegend), CD4-APC (100412, BioLegend), CD3-PerCP/Cyanine5.5 (100217, BioLegend) and NK1.1-APC (156505, BioLegend) antibodies according to different sorting strategies.

(18) Dendritic cells regulate the innate-adaptive balance in lymph nodes for optimal host defenseCellJanuary 22, 2026Jessica Y. Huang, Michael Y. Gerner17A2),BD Biosciences,CAT# 555275 PerCP/Cyanine5.5 Rat monoclonal anti-CD3 (clone 17A2),Biolegend,CAT# 100218 BUV661 Rat monoclonal anti-CD3 (clone 17A2),BD Biosciences,CAT# 741562 BUV737 Rat monoclonal anti-CD3

(19) Targeting senescent EGR1+ B cells enhances immunotherapy efficacy in esophageal squamous cell carcinomaCell Reports MedicineJanuary 20, 2026Pingjing Zhou, Yuchen Zhang, Hongyu Zhang, Pengyuan Zhao, Yifan Guo, Guangyin Zhao, Yiwei Chu, Di Ge, Ronghua Liu, Jie GuII),Thermo Fisher Scientific,Cat# 47-5321-82; RRID:AB_1548783 PE/Cyanine5 anti-mouse CD3,Biolegend,Cat# 100218; RRID:AB_1595492 PerCP/Cyanine5.5 anti-mouse CD19,Biolegend,Cat# 152406; RRID:AB_2629815) Alexa Fluor® 700 anti-mouse CD4

(20) CAR-T triggers TAM reeducation and adaptive anti-tumor response via TREM2 deficiency or CD40 agonistCell Reports MedicineJanuary 20, 2026Ting Liu, Huixin Gao, Zhihui Xi, TianTian Yu, Yimei Gu, Hanbing Mai, Hui Yuan, Yafang Liu, Haikuan Liu, Qiaoxuan Zhang, et al.,Thermo Fisher,Cat#D1306 anti-CD16/CD32,BioLegend,Cat#101302 PerCP/Cyanine5.5 anti-mouse CD3,Biolegend,Cat#100218 PE anti-mouse CD8a,Biolegend,Cat#100708 Brilliant Violet 421 anti-mouse PD-1,Biolegend,Cat#135217 Pacific Blue anti-mouse IFN-γ

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(1)

2/19/2019Anti-Mouse CD3 PerCP/Cy5.5 Antibody Lakshmi BolluThis antibody was used in flow cytometry analysis to detect CD3-positive lymphocytes in spleen and tumor samples.
(1)

10/29/2018Anti-Mouse CD3 Antibody for Flow Cytometry Alexander RaymourOur lab studies immune responses to infection in mouse models. The detection of various immune cell subsets is important in delineating the immune responses in various tissue compartments. The use of ... Read Review
(1)

8/1/2016Good Anti-Mouse CD3 Antibody Tima ThomasWe used this antibody to determine the percentage of CD3 positive T cells in the spleen of Balb/C mouse. Single cell suspensions were isolated from Balb/C mouse splenocytes and were stained with ... Read Review
(1)

3/22/2016Anti-Mouse CD3 Antibody Sachin SamuchiwalI studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice system using anti-mycobacterial ESAT6 binding peptides.
(1)

11/5/2015Anti-CD3 Antibody With Low Level Fluorescence Gurpanna SagguPERCP-Cy5.5 is not the best channel, but was the only one available in my staining panel. So, I decided to use it for a lineage marker like anti-CD3. Usually, the expression is high enough to get a ... Read Review

PerCP/Cyanine5.5 anti-mouse CD3 from BioLegend

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PerCP/Cyanine5.5 anti-mouse CD3 from BioLegend

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