RIP (D94C12) XP ® Rabbit mAb 3493S from Cell Signaling Technology

Product Specs
ItemRIP (D94C12) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $371.00 Inquire
Catalog Number3493S
Quantity100 µl
ApplicationsW, W-S, IP, IF-IC, FC-FP
ReactivityH, M, R, Hm, Mk
TargetRIPK1
HostRabbit
NCBI Gene AliasesAIEFL, IMD57, RIP, RIP-1, RIP1
ClonalityMonoclonal
NCBI Full Gene Namereceptor interacting serine/threonine kinase 1
Research AreaApoptosis
CloneD94C12
Molecular Weight78

Questions and Answers

Can this antibody be used to study necroptosis in keratinocytes?

Yes, the RIP (D94C12) XP® Rabbit mAb has been utilized in studies investigating necroptosis, particularly in keratinocytes. For instance, it was shown that inhibiting RIPK1 with this antibody provided a protective effect against psoriatic inflammation by suppressing necroptosis mediated by RIPK1/RIPK3/MLKL in keratinocytes ().

Is this antibody effective for detecting RIPK1 in mouse models of ischemic stroke?

Yes, the RIP (D94C12) XP® Rabbit mAb has been effectively used in immunoblot assays to detect RIPK1 in mouse models of ischemic stroke. It was cited in a study that explored the roles of various cell death pathways during ischemia/reperfusion, highlighting its utility in understanding RIPK1's involvement in these processes ().

What applications has this antibody been shown to work in?

The RIP (D94C12) XP® Rabbit mAb has been shown to work in several applications, including Western blotting, flow cytometry, immunocytochemistry, immunofluorescence, and immunoprecipitation. This versatility allows researchers to utilize it in various experimental setups to study RIPK1 protein levels and functions.

Does this antibody cross-react with other RIP family members?

No, the RIP (D94C12) XP® Rabbit mAb has not been shown to cross-react with other RIP family members, making it a specific tool for detecting endogenous levels of RIPK1 protein without interference from other related proteins.

Cell Signaling Technology

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Phone: 978-867-2388
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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Transcranial Ultrasound Stimulation Pulsed at 40 Hz Improves Cognition and Neuroinflammation in Female Mice with Alzheimer's DiseaseResearch (Wash D C)April 20, 2026Shasha Yi, Junjie Zou, Xiaofei He, Houminji Chen, Zhengrong Lin, Liying Zhang, Yiyue Zhu, Zejie Zuo, Zehao Chen, Xiquan Hu, et al.antibody,Species,Conc.,Company Phosphorylated RIPK1,Rabbit,"1:1,000",Cell Signaling (31122) RIPK1,Rabbit,"1:1,000",Cell Signaling (3493) Phosphorylated RIPK3,Rabbit,"1:1,000",HUABIO (HA721428) RIPK3,Rabbit,"1:1,000",HUABIO (ER1901-27) Phosphorylated

(2) Schisandrol B protects against lithocholic acid-induced cholestatic liver injury in mice through mitochondrial biogenesisChin MedApril 14, 2026Xiao Yang, Hangfei Liang, Xuan Li, Jianing Tian, Shicheng Fan, Min Huang, Jianbo Wan, Zhong Zuo, Haibiao Guo, Huichang Bi(Cell Signaling Technology, 4494); PINK1 (Abcam, ab23707); PARKIN (Abcam, ab77924); RIP1 (Cell Signaling Technology, 3493); RIP (Phospho Ser166) (Immunoway, YP1467); RIP3 (Cell Signaling Technology, 95702); Phospho-RIP3 (Thr231/Ser232)

(3) Arsenic-Induced PPARγ, with the Coordinated Action of p62, Inhibits Apoptosis and Necroptosis and Activates the DNA Damage Response in A549 Lung Cancer Cells, Leading to CarcinogenesisCellsApril 8, 2026Hak-Ryul Kim, Seon-Hee Ohphospho-Chk1 (#2348), Chk1 (#2360), MLKL (#26539), p21 (#73543), K63-polyubiquitin (#12930), RIP1 (#3493), phospho-γ-H2AX (S139, #2577), PPARγ (2443), and poly/mono-ADP-ribose (#89190) antibodies were purchased

(4) Chemotherapy Enrichment of ID Family Expression Is Associated with IL-6 Signaling in Ovarian CancerCancers (Basel)April 8, 2026Megan Anne Keene, Darren Lighter, Cassandra Brenner, Ixchel Urbano, Katelyn Shelby, Samuel F. Gilbert, Mikella Robinson, Carrie D. HouseDanvers, MA, USA, 1:1000), caspase 8 (MA1-41280, Thermo Fisher, Waltham, MA, USA, 1:1000), and RIP (3493, Cell Signaling Technology, Danvers, MA, USA, 1:1000). Original western blots are presented in File

(5) Curcumin induces and enhances the PARP1‑mediated parthanatos in diffuse large B cell lymphomaOncology LettersMarch 30, 2026Yayun Wang, Ruili Qi, Xuanzhu Zhao, Hanwei Mei, Minghan Qiu, Fengting Liu, Huaqing Wangcat. no. A19684; ABclonal Biotech Co., Ltd.), receptor interacting protein 1 (RIP1; 1:1,000; cat. no. #3493; Cell Signaling Technology, Inc.), receptor interacting protein 3 (RIP3; 1:1,000; cat. no. #13526; Cell Signaling Technology

(6) ADA2-deficient cells exhibit increased levels of cell death and metabolic disturbancesCell Death DiscoveryMarch 23, 2026Lisa Ehlers, Marjon Wouters, Bethany Pillay, Selket Delafontaine, Giorgia Bucciol, Marco Baggio, Mariia Dzhus, Anneleen Hombrouck, Alexandra Damerau, Lien De Somer, et al.(clone: D1L3S, #65746, 1:1000; Cell Signaling Technology; RRID:AB_2799693), anti-RIP (clone: D94C12, #3493, 1:1000; Cell Signaling Technology; RRID:AB_2305314), anti-cleaved caspase-3 (Asp175) (clone: 5A1E,

(7) Caspase-3/7 deficiency results in enhanced intestinal inflammation and reduced tumorigenesisSci AdvMarch 20, 2026Wei Xie, Laura Wyckaert, Mike Vadi, Bruno Verstraeten, Tatyana Divert, Jef Haerinck, Riet De Rycke, Femke Baeke, Mohamed Lamkanfi, Geert Berx, et al.CST), caspase-11 (1:200; NB120-10454, NOVUS), pRIPK1 (Ser166) (1:1000; 53286S, CST), RIPK1 (1:1000; 3493, CST), pRIPK3 (Thr231/Ser232) (1:1000; 57220, CST), pMLKL (Ser345) (1:1000; 37333, CST), MLKL (1:1000;

(8) Context-dependent Activities of Mitrephorone Link Lipid Redirection, Anti-inflammatory Action, and Ferroptosis Control to Hepatocyte ProtectionTheranosticsMarch 17, 2026Lorenz Waltl, Lukas A. Wein, Leonhard Bereuter, Fengting Su, Henriett Barta, Loc Le Xuan, David Holubek, Zahra Mahmoudi Eshkaftaki, Katharina Puskac, Anita Siller, et al.anti-phospho-RIPK1 (Ser166) (1:500, D1L3S, #65746; Cell Signaling, Danvers, MA), rabbit anti-RIPK1 (1:500, D94C12, #3493; Cell Signaling), rabbit monoclonal anti-β-actin (1:1000; #4970; Cell Signaling), or mouse monoclonal

(9) Study on the Function and Mechanism of Neutrophil Extracellular Traps in Regulating Necroptosis Following Traumatic Brain InjuryBrain BehavMarch 10, 2026Ao Li, Tian-Wei Pei, Hao Qi, Li-Biao Song, Juan Fang, Zhi-Song Ding, Tao Chen(1:1000, ab208670, Abcam), Bcl‐2 (1:1000, A0208, Abclonal), Bax (1:1000, A19684, Abclonal), RIP1 (1:1000, #3493, Cell Signaling Technology), RIP3 (1:1000, #95702, Cell Signaling Technology), MLKL (1:1000, #37705,

(10) Toll-like receptor signaling outcome is determined by the stoichiometry of the endogenous TRIFosomeSci AdvMarch 6, 2026Martin C. Moncrieffe, Prasanna Suresh, Joe Boyle, Yuhao Cui, Bharti Nawalpuri, Brett Verstak, Yu P. Zhang, Ziwei Zhang, Marcus Taylor, Edward H. Egelman, et al.Tween 20) wash performed between these two steps. Either an RIP rabbit monoclonal antibody (D94C12, no. 3493, Cell Signaling) or a rabbit IgG isotype control (Abcam, EPR25A) was then introduced to independent

(11) Macrophage metabolic exhaustion and PANoptotic cell death drive chronic tissue inflammation in rheumatoid arthritisImmunityMarch 6, 2026Tao Huang, Yoshinori Takashima, Jitendra Kumar, Jose Morales, Kevin Le, Zhensheng Hu, Selene Rubino, Robert T. Trousdale, Gerald J. Berry, Jorg J. Goronzy, et al.,Cell Signaling Technology,Cat# 9661; RRID: AB_2732034 RIP (D94C12) XP® Rabbit mAb,Cell Signaling Technology,Cat# 3493; RRID: AB_2305314 ASC/TMS1 Polyclonal antibody,proteintech,Cat# 10500-1-AP; RRID: AB_2174862 ,, Bacterial

(12) Early Development of Ocadusertib, a Selective Receptor-Interacting Serine/Threonine-Protein Kinase 1 InhibitorClin Transl SciMarch 6, 2026Simon J. Shaw, Vanessa C. Taylor, Jonathan T. Sims, Stephen Ho, Chrystelle Lamagna, Lucy Yan, Nicholas C. Reising, Andrew Chow, Eric P. Hanson, Andrew C. Vendel, et al.(UB; 2% BSA in PBST) for 1 h. Plates were washed, then 80 ng/mL anti‐RIPK1 D94C12 XP (Cell Signaling #3493) in UB was added for 1 h. Plates were washed, then 110 ng/mL anti‐rabbit‐HRP (Southern Biotech #4049‐05)

(13) Amomum tsaoko extract from Nujiang alleviates DSS-induced colitis through inhibiting necroptosisFrontiers in Cell and Developmental BiologyFebruary 26, 2026Yuanyuan Wang, Keyi Lu, Yuhang Gong, Yanna Shao, Siqi Liu, Yuan Fang, Yifan Shi, Erping Xu, Yanqiong Yang, Si Yuan, et al.(HY-15989A) was acquired from Med Chem Express. Z-VAD-fmk (T6013) was bought from TargetMol. Anti-RIPK1 (3493S) and human-specific anti-phospho-RIPK1 (65746S) was bought from Cell Signaling Technology. The BCA protein assay kit

(14) Mitochondrial permeability transition pore desensitization by a novel dispiranic derivative prevents cardiac reperfusion injuryRedox BiologyFebruary 19, 2026Giampaolo Morciano, Gaia Pedriali, Giulia Turrin, Cristina Algieri, Esther Densu Agyapong, Debora La Mantia, Chiara Bernardini, Giorgia Canini, Anna Fantinati, Elena Nicoletta Colarusso, et al.PARP (Cell signaling, 9541, 1:1000), Caspase 3 (Cell signaling, 9662, 1:1000), RIP (Cell signaling, 3493, 1:1000), β-Actin (Merck, A1978, 1:5000), ATP5A (Abcam, ab14748, 1:5000). The membranes were then treated

(15) The metabolite α-ketoglutarate induces AIM2-dependent PANoptosis through demethylase TET2Cell Communication and SignalingFebruary 16, 2026Yi Li, Qing Tu, Chenchen Liu, Jiamin Ma, Yuwei Chen, Lin Wang, Ying Chen, Jinbao Li, Jiali Zhu, Yun Zou, et al.,Cell Signaling Technology, #8438), receptor-interacting protein kinase 1 (RIPK-1) (1:2000,Cell Signaling Technology, #3493), phosphorylated RIPK1 (pRIPK-1) (1:1000,Cell Signaling Technology, #31,122), mixed lineage kinase domain-like

(16) NLK facilitates Caspase-8 activation to drive macrophage PANoptosis in sepsisClin Transl MedFebruary 11, 2026Yun Xia, Ren-Qi Yao, Hui-Fan Liu, Guo-Qing Jing, Delida Aidebaike, Jing Zuo, Shi-Qi Wan, Die Wu, Peng-Yue Zhao, Hai-Long Gong, et al.(#96662), Caspase‐7 (#9492), MLKL (#37705), p‐MLKL (#37333), RIPK3 (#95702), p‐RIPK3 (#91702S), RIPK1 (#3493), NLRP3 (#15101), GSDMD (#39754), and ASC (#67824) were purchased from Cell Signaling Technology (Danvers,

(17) Dietary Polyphenols Curcumin and Resveratrol Exert Selective Anticancer Effects in Melanoma CellsNutrientsFebruary 6, 2026Moon-Kyun Cho, Yeji Lee, Ki Dam Kim, Min Hyuk Choi, Sang-Han Lee, Dongsic Choi, Hae-Seon Nam, Yoon-Jin Lee,Cell Signaling Technology,#5625,1:500 Phospho-RIP,78,Rabbit,Cell Signaling Technology,#65746,1:500 RIP,78,Rabbit,Cell Signaling Technology,#3493,1:500 Phospho-MLKL,54,Rabbit,Cell Signaling Technology,#91689,1:500 MLKL,54,Rabbit,Cell Signaling Technology

(18) The C3-C3aR axis drives rotenone-induced cognitive damage via synaptic engulfment, dark microglia and PANoptosisRedox BiologyJanuary 30, 2026Jianing Liu, Liyan Hou, Yu Ma, Lu Tian, Jau-Shyong Hong, Jie Zhao, Qingshan Wang(9101S), p-ERK (4695T), p65 (8242T), p-p65 (3033S), IκBα (2859T), p-IκBα (4814T), RIP1 (53286S), p-RIP1 (3493S), MLKL (37333S), p-MLKL (37705S), and RIP3 (95702S) antibodies from Cell Signaling Technology (Danvers,

(19) Rhein protects against renal aging and fibrotic injury by multiple targets through inhibition of TNF-α-mediated autophagy and necroptosis crosstalkFrontiers in PharmacologyJanuary 26, 2026Yue Tu, Wenjie Liu, Tianqing Sang, Liuyunxin Pan, Wei Wu, Qijun Fang, Yinglu Liu, Buhui Liu, Yigang Wan(1:1000, #23214), anti-beclin1 (1:1000, #3495), anti-LC3A/B (1:1000, #12741), anti-RIPK1 (1:1000, #3493), anti-RIPK3 (1:1000, #15828), anti-caspase-8 (1:1000, #4790) and anti-cleaved-caspase-8 (1:1000, #8592)

(20) SIGLEC12 mediates plasma membrane rupture during necroptotic cell deathNatureJanuary 8, 2026Hyunjin Noh, Zeena Hashem, Elena Boms, Ayaz Najafovreagents The following antibodies were used in this study: RIPK1 (Cell Signaling Technology, catalogue no. 3493); p-hRIPK1 (Cell Signaling Technology, catalogue no. 65746); hRIPK3 (Cell Signaling Technology, catalogue

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RIP (D94C12) XP ® Rabbit mAb

RIP (D94C12) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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RIP (D94C12) XP ® Rabbit mAb from Cell Signaling Technology

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