Fig 1: Effect of depletion of CEACAM6. (A) Changes over 3 days in GBC-SD and SGC-996 cells measured by CCK8 assay. (B and C) Colony formation analysis of the cell colony number after transfection with CEACAM6 siRNA and NC siRNA in GBC-SD cells and in SGC-996 cells. *P<0.05, **P<0.01 vs. NC group. CEACAM6, carcinoembryonic antigen-related cell adhesion molecule 6; NC, negative control; siRNA, small interfering RNA.
Fig 2: Effect of CEACAM6 knockdown on wound healing. (A) Wound-healing analysis of cell migration after transfection of CEACAM6 siRNA and NC siRNA in GBC-SD and SGC-996 cells (magnification, ×50). (B) Relative migration is shown in relevant graph bars. **P<0.01 vs. NC group. CEACAM6, carcinoembryonic antigen-related cell adhesion molecule 6; NC, negative control; siRNA, small interfering RNA.
Fig 3: Kaplan-Meier analysis of the association of CEACAM6 expression with overall survival in patients with gall bladder cancer (n=68). CEACAM6, carcinoembryonic antigen-related cell adhesion molecule 6.
Fig 4: Effect of CEACAM6 depletion on cell migration. (A and C) Transwell analysis of cell migration potential after transfection of SGC-996 and GBC-SD cells with siRNA targeting CEACAM6 and NC siRNA (magnification, ×200). (B and D) Cell counts are shown in relevant graph bars. **P<0.01 vs. NC group. CEACAM6, carcinoembryonic antigen-related cell adhesion molecule 6; NC, negative control; siRNA, small interfering RNA.
Fig 5: Forest plots showing hazard ratios for overall survival: CEACAM6 overall (a) and by subgroups based on disease site (b). Hazard ratios for each study are represented by squares: the size of the square represents the weight of the study in the meta-analysis; the horizontal line passing through the square represents the 95% confidence interval. All statistical tests were two-sided. The diamonds represent the estimated pooled effect. Test for overall effect based on z-test. All p values are two-sided. (a) CEACAM6 OS Overall. (b) CEACAM6 OS by disease site.CI, confidence interval; OR, odds ratio.
Supplier Page from Abcam for Anti-CEACAM6 antibody [9A6]