Flow Cytometry Permeabilization/Wash Buffer I (1X) from R&D Systems, a Bio-Techne Brand

Product Specs
ItemFlow Cytometry Permeabilization/Wash Buffer I (1X)
CompanyR&D Systems, a Bio-Techne Brand
Price $179.00Supplier Page View Company Product Page
Catalog NumberFC005
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
ApplicationsFlow Cytometry
Quantity250 mL

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Aurantio-obtusin modulates Wilms Tumour 1 within the breast tumour microenvironment reducing immunosuppression and tumour growthCell Commun SignalJuly 1, 2025Rui Li, Dómhnall J O’Connor, Barry Digby, Pilib Ó. Broin, Xiao Hu, Ning Ge, Paul G. Loftus, Vatsal Kumar, Eoin McEvoy, Stephen J. Elliman, et al.fixation buffer (R&D systems, FC004) for 10 min and permeabilized with 200 µl of wash buffer (R&D systems, FC005) for 20 min at room temperature in dark. Cells were then blocked with Fc block (BD Biosciences, 564,220;

(2) A new fluorescent probe for the visualization of progerinBioorganic chemistryJanuary 1, 2024Macicior J, Fernández D, Ortega-Gutiérrez S.

(3) The Impact of Biomaterial Surface Properties on Engineering Neural Tissue for Spinal Cord RegenerationInternational journal of molecular sciencesSeptember 4, 2023da Silva VA, Bobotis BC, Correia FF, Lima-Vasconcellos TH, Chiarantin GMD, De La Vega L, Lombello CB, Willerth SM, Malmonge SM, Paschon V, et al.room temperature, then washed and resuspended in flow cytometry permeabilization/wash buffer I (1X) (FC005, R&D Systems, Minneapolis, MN, USA). Marker antibodies, TUJ1 (60052, Stemcell Technologies, Vancouver,

(4) Acquired CFTR dysfunction and dense distribution of ionocytes in nasal mucosa of children with CRSEuropean Archives of Oto-Rhino-LaryngologyJuly 1, 2023Yang Han, Chao Jia, Tieshan Wang, Pengpeng Wang, Wenjing Liu, Yu Qin, Siyu Cai, Xiaojian Yang, Wei Zhang, Yuwei Liu, et al.were re-suspended with 200 μl Flow Cytometry Permeabilization/Wash Buffer (R&D Systems®, Catalog # FC005). NBP2-70747AF488 (W001-071624-AF488), PE/Cyanine7 anti-human-CD45 (304061) and NBP2-54509AF647 (1 μg)

(5) PAX9 Is Involved in Periodontal Ligament Stem Cell-like Differentiation of Human-Induced Pluripotent Stem Cells by Regulating Extracellular MatrixBiomedicinesSeptember 22, 2022Sugiura R, Hamano S, Tomokiyo A, Hasegawa D, Yoshida S, Sugii H, Fujino S, Adachi O, Kadowaki M, Yamashita D, et al.

(6) Methods for culturing adult CNS neurons reveal a CNS conditioning effectCell Rep MethodsJuly 18, 2022van Niekerk EA, Kawaguchi R, Marques de Freria C, Groeniger K, Marchetto MC, Dupraz S, Bradke F, Geschwind DH, Gage FH, Tuszynski MH.as above. Pellet was resuspended in 200 μL flow cytometry permeabilization/wash buffer (R&D systems, FC005) and Tuj1-APC antibody (Sony, 4606095) was added at 1:50 dilution; CTIP2-488 antibody (Abcam, AB18465)

(7) Wnt Site Signaling Inhibitor Secreted Frizzled-Related Protein 3 Protects Mitral Valve Endothelium From Myocardial Infarction-Induced Endothelial-to-Mesenchymal TransitionJournal of the American Heart AssociationApril 5, 2022Alvandi Z, Nagata Y, Passos LSA, Hashemi Gheinani A, Guerrero JL, Wylie-Sears J, Romero DC, Morris BA, Sullivan SM, Yaghoubian KM, et al.Minneapolis, MN; No. FC004) and labeled in Flow Cytometry Permeabilization/Wash Buffer I (R&D Systems; No. FC005) for 45 minutes (100 000 cells/100 µL buffer I). Anti–VE‐cadherin antibody (1:50; Aviva Systems Biology,

(8) A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosisNature communicationsSeptember 30, 2021Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, et al.

(9) Searching for new molecular markers for cells obtained from abdominal aortic aneurysmJournal of Applied GeneticsJune 2, 2021Marta Lesiak, Aleksandra Augusciak-Duma, Karolina L. Stepien, Agnieszka Fus-Kujawa, Malwina Botor, Aleksander L. Sieronto a final density of 106 cells in 200 µl of Flow Cytometry Permeabilization/Wash Buffer I (cat. no. FC005, R&D Systems, Minneapolis, MN, USA). Fluorochrome-conjugated antibodies against cytoplasmic antigens

(10) β2M Signals Monocytes Through Non-Canonical TGFβ Receptor Signal TransductionCirculation researchMarch 5, 2021Hilt ZT, Maurya P, Tesoro L, Pariser DN, Ture SK, Cleary SJ, Looney MR, McGrath KE, Morrell CN.intermittently. Cells were resuspended into 200 μL of Flow Cytometry Permeabilization/Wash Buffer (FC005, R&D Systems) and stained with intracellular antibodies (pJNK, pSMAD2/3, NFκB, NR4A1, SMAD4) for 30

(11) Overcoming bioprocess bottlenecks in the large-scale expansion of high-quality hiPSC aggregates in vertical-wheel stirred suspension bioreactorsStem cell research & therapyJanuary 13, 2021Borys BS, Dang T, So T, Rohani L, Revay T, Walsh T, Thompson M, Argiropoulos B, Rancourt DE, Jung S, et al.Aggregate samples were then rinsed twice with PBS and resuspended in 200-μL permeabilization buffer (Cat#FC005, R&D Systems) with 1 μg/106 cells antibody stain and 1 μM/106 cells nuclei stain and incubated for 3

(12) A novel cardiac differentiation method of a large number and uniformly-sized spheroids using microfabricated culture vesselsRegenerative therapyDecember 1, 2020Miwa T, Idiris A, Kumagai H.

(13) Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm StateCell stem cellNovember 5, 2020Zhang L, Avery J, Yin A, Singh AM, Cliff TS, Yin H, Dalton S.

(14) Optimized serial expansion of human induced pluripotent stem cells using low-density inoculation to generate clinically relevant quantities in vertical-wheel bioreactorsStem cells translational medicineSeptember 1, 2020Borys BS, So T, Colter J, Dang T, Roberts EL, Revay T, Larijani L, Krawetz R, Lewis I, Argiropoulos B, et al.Dissociated cells were rinsed twice with PBS then resuspended in 200 μL of permeabilization buffer (Cat#FC005, R&D Systems) with 1 μg/106 cells antibody stain and 1 μM/106 cells nuclei stain and incubated for 1

(15) Multi-model functionalization of disease-associated PTEN missense mutations identifies multiple molecular mechanisms underlying protein dysfunctionNature communicationsApril 29, 2020Post KL, Belmadani M, Ganguly P, Meili F, Dingwall R, McDiarmid TA, Meyers WM, Herrington C, Young BP, Callaghan DB, et al.for 10 min in 3.2% PFA. Cells were permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005, R&D Systems) and stained with Rabbit anti-pAKT (1:100; Cell Signaling, Ser473, cat. #9271) and Mouse

(16) Acetylcholine decreases formation of myofibroblasts and excessive extracellular matrix production in an in vitro human corneal fibrosis modelJournal of cellular and molecular medicineApril 1, 2020Słoniecka M, Danielson P.PBS and the pellet was resuspended in 200 µL of Flow Cytometry Permeabilization/Wash Buffer I (R&D, #FC005). 10 µL of human alpha‐smooth muscle actin PE‐conjugated antibody (R&D Systems # IC1420P) was added.

(17) Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stressThe Journal of biological chemistryNovember 8, 2019Merriman C, Fu D.

(18) Role of vimentin in modulating immune cell apoptosis and inflammatory responses in sepsisScientific reportsApril 5, 2019Su L, Pan P, Yan P, Long Y, Zhou X, Wang X, Zhou R, Wen B, Xie L, Liu D.resuspended in 200 μL of the Flow Cytometry Permeabilization/Wash Buffer I (R&D Systems, Catalog # FC005). FITC-conjugated mouse anti-human-vimentin (ab128507, Abcam, Cambridge, MA) was added to the cells

(19) Generation of iPSCs from Jaw Periosteal Cells Using Self-Replicating RNAInternational journal of molecular sciencesApril 3, 2019Umrath F, Steinle H, Weber M, Wendel HP, Reinert S, Alexander D, Avci-Adali M.

(20) Generation of two iPSC lines with either a heterozygous V717I or a heterozygous KM670/671NL mutation in the APP geneStem cell researchJanuary 1, 2019Frederiksen HR, Holst B, Ramakrishna S, Muddashetty R, Schmid B, Freude K.

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Flow Cytometry Permeabilization/Wash Buffer I (1X) from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Flow Cytometry Permeabilization/Wash Buffer I (1X) from R&D Systems, a Bio-Techne Brand

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