Flow Cytometry Fixation Buffer (1X) from R&D Systems, a Bio-Techne Brand

Product Specs
ItemFlow Cytometry Fixation Buffer (1X)
CompanyR&D Systems, a Bio-Techne Brand
Price $179.00Supplier Page View Company Product Page
Catalog NumberFC004
Quantity100 mL
ApplicationsFlow Cytometry
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) An Anti-BCMA Affibody Affinity Protein for Therapeutic and Diagnostic Use in Multiple MyelomaInternational Journal of Molecular SciencesMay 28, 2025Kim Anh Giang, Johan Nilvebrant, Hao Liu, Harpa Káradóttir, Yumei Diao, Stefan Svensson Gelius, Per-Åke Nygrentwice with Flow staining buffer, fixed with Fixation buffer (Flow Cytometry Fixation Buffer, cat. no. FC004, R&D Systems, Minneapolis, MN, USA) for 10 min at RT, then washed again and resuspended in Flow staining

(2) The Impact of Biomaterial Surface Properties on Engineering Neural Tissue for Spinal Cord RegenerationInternational journal of molecular sciencesSeptember 4, 2023da Silva VA, Bobotis BC, Correia FF, Lima-Vasconcellos TH, Chiarantin GMD, De La Vega L, Lombello CB, Willerth SM, Malmonge SM, Paschon V, et al.and centrifuging at 600 g for 5 min. The cells were then fixed with flow cytometry fixation buffer (FC004, R&D Systems, Minneapolis, MN, USA) and incubated for 30 min at room temperature, then washed and resuspended

(3) Long-term changes of Th17 and regulatory T cells in peripheral blood of dogs with spinal cord injury after intervertebral disc herniationBMC veterinary researchJuly 22, 2023Wesolowski M, Can P, Warzecha K, Freise F, Carlson R, Neßler J, Tipold A.

(4) Acquired CFTR dysfunction and dense distribution of ionocytes in nasal mucosa of children with CRSEuropean Archives of Oto-Rhino-LaryngologyJuly 1, 2023Yang Han, Chao Jia, Tieshan Wang, Pengpeng Wang, Wenjing Liu, Yu Qin, Siyu Cai, Xiaojian Yang, Wei Zhang, Yuwei Liu, et al.washed with PBS buffer. Fixed cells 0.5 ml Flow Cytometry Fixation Buffer (R&D Systems®, Catalog # FC004) resuscitated 5 × 105 cells, incubated at room temperature for 10 min and swirled the cells continuously.

(5) Methods for culturing adult CNS neurons reveal a CNS conditioning effectCell Rep MethodsJuly 18, 2022van Niekerk EA, Kawaguchi R, Marques de Freria C, Groeniger K, Marchetto MC, Dupraz S, Bradke F, Geschwind DH, Gage FH, Tuszynski MH.centrifuged at 300xg for 10 min and resuspended in 500 μL flow cytometry fixation buffer (R&D systems, FC004) and incubated for 10 min at room temp. Cells were washed with PBS and centrifuged again as above. Pellet

(6) Wnt Site Signaling Inhibitor Secreted Frizzled-Related Protein 3 Protects Mitral Valve Endothelium From Myocardial Infarction-Induced Endothelial-to-Mesenchymal TransitionJournal of the American Heart AssociationApril 5, 2022Alvandi Z, Nagata Y, Passos LSA, Hashemi Gheinani A, Guerrero JL, Wylie-Sears J, Romero DC, Morris BA, Sullivan SM, Yaghoubian KM, et al.The cell suspension was fixed using Flow Cytometry Fixation Buffer (R&D Systems, Minneapolis, MN; No. FC004) and labeled in Flow Cytometry Permeabilization/Wash Buffer I (R&D Systems; No. FC005) for 45 minutes

(7) Systemic T Cell Exhaustion Dynamics Is Linked to Early High Mobility Group Box Protein 1 (HMGB1) Driven Hyper-Inflammation in a Polytrauma Rat ModelCellsJune 30, 2021Muire PJ, Schwacha MG, Wenke JC.

(8) Evaluation of the involvement of Th17-cells in the pathogenesis of canine spinal cord injuryPloS oneJanuary 1, 2021Kämpe A, Knebel A, Carlson R, Rohn K, Tipold A.California, USA",10270–106,- Flow Cytometry Fixation Buffer (1X),"R&D Systems®, Minneapolis, USA",FC004,- Goat Anti-Mouse IgG MicroBeads,"MACS Miltenyi Biotec GmbH, Bergisch Gladbach, Germany",130-048-042,1: 5

(9) Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm StateCell stem cellNovember 5, 2020Zhang L, Avery J, Yin A, Singh AM, Cliff TS, Yin H, Dalton S.

(10) Extracellular Vesicle-Mediated In Vitro Transcribed mRNA Delivery for Treatment of HER2⁺ Breast Cancer Xenografts in Mice by Prodrug CB1954 without General ToxicityMolecular cancer therapeuticsMarch 1, 2020Forterre AV, Wang JH, Delcayre A, Kim K, Green C, Pegram MD, Jeffrey SS, Matin AC.

(11) Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stressThe Journal of biological chemistryNovember 8, 2019Merriman C, Fu D.

(12) c-Myc shuttled by tumour-derived extracellular vesicles promotes lung bronchial cell proliferation through miR-19b and miR-92aCell death & diseaseOctober 7, 2019Borzi C, Calzolari L, Ferretti AM, Caleca L, Pastorino U, Sozzi G, Fortunato O.

(13) Generation of iPSCs by Nonintegrative RNA-Based Reprogramming Techniques: Benefits of Self-Replicating RNA versus Synthetic mRNAStem cells internationalJanuary 1, 2019Steinle H, Weber M, Behring A, Mau-Holzmann U, Schlensak C, Wendel HP, Avci-Adali M.

(14) E3 Ubiquitin Ligase RNF125 Activates Interleukin-36 Receptor Signaling and Contributes to Its TurnoverJournal of innate immunityJanuary 1, 2018Saha SS, Caviness G, Yi G, Raymond EL, Mbow ML, Kao CC.and Ranjith-Kumar et al. [17]. NCI cells were treated with fixation buffer (R&D Systems; catalog No. FC004) followed by the permeabilization buffer I (R&D Systems; catalog No. FC005). The cells were stained

(15) CD45 Expression in Mitral Valve Endothelial Cells After Myocardial InfarctionCirculation ResearchNovember 11, 2016Joyce Bischoff et al.suspension of MV cells. Isolated cells were fixed using Flow Cytometry Fixation Buffer (R&D Systems, MN; #FC004) and labeled in Flow Cytometry Permeabilization/Wash Buffer I (R&D Systems; #FC005) for 45 minutes (100

(16) Signal Transduction and Intracellular Trafficking by the Interleukin 36 ReceptorThe Journal of biological chemistrySeptember 25, 2015Saha SS, Singh D, Raymond EL, Ganesan R, Caviness G, Grimaldi C, Woska JR Jr, Mennerich D, Brown SE, Mbow ML, et al.

(17) The Spiegelmer NOX-A12, a novel CXCL12 inhibitor, interferes with chronic lymphocytic leukemia cell motility and causes chemosensitizationBloodFebruary 13, 2014Hoellenriegel J, Zboralski D, Maasch C, Rosin NY, Wierda WG, Keating MJ, Kruschinski A, Burger JA.

(18) Coxsackievirus B3-induced cellular protrusions: structural characteristics and functional competenceJournal of virologyJuly 1, 2011Paloheimo O, Ihalainen TO, Tauriainen S, Välilehto O, Kirjavainen S, Niskanen EA, Laakkonen JP, Hyöty H, Vihinen-Ranta M.

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Flow Cytometry Fixation Buffer (1X) from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Flow Cytometry Fixation Buffer (1X) from R&D Systems, a Bio-Techne Brand

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