Flow Cytometry Staining Buffer (1X) FC001 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemFlow Cytometry Staining Buffer (1X)
CompanyR&D Systems, a Bio-Techne Brand
Price $179.00Supplier Page View Company Product Page
Catalog NumberFC001
Quantity500 mL
ApplicationsFlow Cytometry
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Focused Ultrasound Thermal Ablation and CD40 Agonism Reprograms Breast Tumor Immunity to Drive Regression and MemorybioRxivMarch 4, 2026Zehra E. F. Demir, AeRyon Kim, Beyzanur G. Ak, Micaiah S.J. Lee, Thomas Sherlock, Stefanyda O. Maslova, Andrew T. Thede, Matthew R. DeWitt, Melanie R. Rutkowski, Natasha D. Sheybaniat 4°C, then centrifuged and washed twice with 1X Flow Cytometry Staining Buffer (FACS; R&D Systems #FC001). Surface staining was performed in Brilliant Stain Buffer (BD Biosciences #566349) for 30 mins at 4°C

(2) IL-6R blockade with tocilizumab disrupts pericyte- and tumor cell-driven IL-6/STAT3 signaling, enhancing docetaxel efficacy in ER+ breast cancerbioRxivJanuary 31, 2026Roza Kamila Przanowska, Jacky Gomez-Villa, Victoria J. Liu, Natalia Antonides-Jensen, Lavanya Visvabharathy, John C. Alverdy, Sonia L. Hernandez, Samantha S. Yeeneutralize the 0.25% or 0.05% trypsin-EDTA, respectively, and then resuspended in flow buffer (Bio-Techne #FC001). Cells were spun down at 1000 rpm for 3 to 4 minutes and resuspended in 100 μL volume flow buffer in

(3) Metabolic Profiling Reveals a Glycolytic Shift and an IRG1/Itaconate/NF2L2 Axis Regulating Neurotoxic Oxidative Stress in Inflammatory MicrogliaJournal of NeurochemistrySeptember 5, 2025Pinelopi Engskog‐Vlachos, Mikael K. R. Engskog, Martin Skandik, Kathleen Grabert, Noah Moruzzi, Marie‐Kim St‐Pierre, Ahmed M. Osman, Theodora Sylaidi, Klas Blomgren, Per‐Olof Berggren, et al.× g for 5 min at room temperature. Pellets were resuspended in a flow cytometry buffer (Biotechne, #FC001). Cells collected from all culture flasks were pooled and centrifuged as above. The cell pellet was

(4) Loss of integrin alpha7-mediated signaling induces a dendritic cell-like phenotype in macrophages cultured on laminin-211/221 isoformsJournal of Biological ChemistryJune 25, 2025Nagako Yoshiba, Tomoki Maekawa, Kiyotoshi Sekiguchi, Masaru Kaku, Kridtapat Sirisereephap, Meircurius Surboyo, Yurie Sato-Yamada, Andrea Rosenkranz, Akihiro Hosoya, Naoto Ohkura, et al.collected by heavy pipetting and washed three times with Flow Cytometry Staining Buffer (R&D System, FC001). Fluorescence was measured using flow cytometry. RNA sequencing A next-generation sequencing library

(5) Positive autoregulation of Sox17 is necessary for gallbladder and extrahepatic biliary duct formationDevelopmentJanuary 15, 2025Linh T. Trinh, Ryan R. Finnel, Anna B. Osipovich, Jessica R. Musselman, Leesa L. Sampson, Christopher V. E. Wright, Mark A. Magnusonat 1000 rpm (200 g) for 3 min at 4°C and resuspended in flow cytometry staining buffer (R&D Systems, FC001) containing 2 U/ml DNase I (Flow/DNaseI buffer). Fc-blocking occurred for all samples with mouse IgG

(6) Cannabidiol Enhances Atezolizumab Efficacy by Upregulating PD-L1 Expression via the cGAS–STING Pathway in Triple-Negative Breast Cancer CellsCancer Immunology ResearchDecember 3, 2024Bu Gyeom Kim, Bo Ram Kim, Dae Yeong Kim, Woo Young Kim, Sanghee Kang, Sun Il Lee, Sang Cheul Ohper minute (rpm) for 5 minutes. Cells were resuspended in 200 μL 1× flow cytometry staining buffer (FC001, R&D Systems) and incubated with PE-conjugated antibodies against PD-L1 or isotype control for 30 minutes

(7) Rapid and robust isolation of microglia and vascular cells from brain subregions for integrative single-cell analysesHeliyonAugust 30, 2024Efthalia Preka, Alejandro Lastra Romero, Ying Sun, Yara Onetti Vilalta, Thea Seitz, Adamantia Fragkopoulou, Christer Betsholtz, Ahmed M. Osman, Klas Blomgrencarefully discarded, and the cell pellet was resuspended in 1 ml flow cytometry buffer (R&D Systems #FC001). At this step, samples from the three animals were pooled into one 15 ml conical tube and centrifuged

(8) LINE-1 RNA triggers matrix formation in bone cells via a PKR-mediated inflammatory responseThe EMBO JournalJuly 1, 2024Arianna Mangiavacchi, Gabriele Morelli, Sjur Reppe, Alfonso Saera-Vila, Peng Liu, Benjamin Eggerschwiler, Huoming Zhang, Dalila Bensaddek, Elisa A Casanova, Carolina Medina Gomez, et al.then washed and resuspended in 100 μl of Flow Cytometry Staining Buffer (R&D System, USA, Cat. No. FC001). About 10 μl of 1 mg/ml Propidium iodide (PI) staining solution (Invitrogen, USA, Cat. No. P3566) was

(9) Define Critical Parameters of Trastuzumab-Mediated ADCC Assays via Assay Optimization Processes, Focusing on the Impact of Cryopreserved Effector Cells on Assay PerformanceCancersJune 27, 2024Peng H, Endo Y, Wu WJ.Briefly, PBMCs or NK cells (5 × 105 cells per sample) were washed with Staining Buffer (R&D Systems, Cat# FC001), Fc-blocked with BD Pharmingen™ Human BD Fc Block™ (BD, Cat# BDB564219) at 1 µg/106 cells in 100 µL

(10) Sex Dependent Disparities in the Central Innate Immune Response after Moderate Spinal Cord Contusion in RatCellsApril 6, 2024Ghosh M, Lee J, Burke AN, Strong TA, Sagen J, Pearse DD.purified microglia, the cells were suspended in flow cytometry staining buffer (1×) (R&D systems, Cat No. FC001, Minneapolis, MN, USA) and treated with purified mouse anti-rat CD32 (1 μg IgG per one million cells:

(11) Developing Folate-Conjugated miR-34a Therapeutic for Prostate Cancer: Challenges and PromisesInternational Journal of Molecular SciencesFebruary 9, 2024Wen (Jess) Li, Yunfei Wang, Xiaozhuo Liu, Shan Wu, Moyi Wang, Steven G. Turowski, Joseph A. Spernyak, Amanda Tracz, Ahmed M. Abdelaal, Kasireddy Sudarshan, et al.supplemented with 0.5% BSA and aliquoted to a density of 5 × 106 cells/mL in flow cytometry staining buffer (FC001, R&D Systems, Minneapolis, MN, USA). Next, flow cytometric analyses were performed following standard

(12) RIPK1 is aberrantly expressed in multiple B-cell cancers and implicated in the underlying pathogenesisDiscover OncologyJuly 18, 2023Baoyu Wu, Jingyu Li, Han Wang, Jianguo Liu, Jiayong Li, Fang Sun, Dong chuan Fengof 300×g. Furthermore, cells were resuspended using 100 μL of Flow Cytometry Staining Buffer (Cat: # FC001, R&D systems). 10 μL of PI staining solution was added to a control tube of otherwise unstained cells

(13) Acquired CFTR dysfunction and dense distribution of ionocytes in nasal mucosa of children with CRSEuropean Archives of Oto-Rhino-LaryngologyJuly 1, 2023Yang Han, Chao Jia, Tieshan Wang, Pengpeng Wang, Wenjing Liu, Yu Qin, Siyu Cai, Xiaojian Yang, Wei Zhang, Yuwei Liu, et al.was discarded. After resuspension with 400 μl Flow Cytometry Staining Buffer (R&D Systems®, Catalog # FC001), the number of ionocytes and the cells expressing CFTR in the nasal mucosa were analyzed using flow cytometry

(14) GSK-3 inhibitor elraglusib enhances tumor-infiltrating immune cell activation in tumor biopsies and synergizes with anti-PD-L1 in a murine model of colorectal cancerbioRxiv : the preprint server for biologyFebruary 7, 2023Huntington KE, Louie AD, Srinivasan PR, Schorl C, Lu S, Silverberg D, Newhouse D, Wu Z, Zhou L, Borden BA, et al.

(15) Differential miRNA Expression Contributes to Emergence of Multiple Cancer Stem Cell Subpopulations in Human Colorectal CancerbioRxiv : the preprint server for biologyFebruary 6, 2023Stark VA, Facey COB, Opdenaker L, Fields JZ, Boman BM.Cells were pelleted and then resuspended in 1 mL of Flow Cytometry Staining Buffer 1X (R&D Systems #FC001) for blocking. Cells were blocked for 1 hour on ice in the dark. After blocking, the cells were spun

(16) Differential miRNA Expression Contributes to Emergence of Multiple Cancer Stem Cell Subpopulations in Human Colorectal CancerJournal of stem cell research & therapyJanuary 1, 2023Stark VA, Facey COB, Opdenaker LM, Fields JZ, Boman BM.tube. Cells were pelleted and then suspended in 1 mL of Flow Cytometry Staining Buffer 1X (R&D Systems #FC001) for blocking. Cells were blocked for 1 hour on ice in the dark. After blocking, the cells were spun

(17) Interference with pathways activated by topoisomerase inhibition alters the surface expression of PD-L1 and MHC I in colon cancer cellsOncology lettersJanuary 1, 2023Hassan M, Trung V, Bedi D, Shaddox S, Gunturu D, Yates C, Datta P, Samuel T.(St. Louis, MO). AZD7762 (Enzo, ENZ-CHM185-0005) from ENZO. Flow Cytometry Staining Buffer (1X) (Cat# FC001), Mouse PE-IgG2a (R&D IC003p), and Human HLA Class I (MHC I) Phycoerythrin mAb (Clone W6/32) were purchased

(18) Mutant MESD links cellular stress to type I collagen aggregation in osteogenesis imperfecta type XXMatrix biology : journal of the International Society for Matrix BiologyJanuary 1, 2023Ghosh DK, Udupa P, Shrikondawar AN, Bhavani GS, Shah H, Ranjan A, Girisha KM.15400054) and then washed with PBS. Cells were resuspended in Flow Cytometry Staining Buffer (R&D systems, FC001) containing 1 μ g/ml propidium iodide (SA, P4170). Cells were kept in this solution for 30 min at 37°C

(19) Dentin Particulate for Bone Regeneration: An In Vitro StudyInternational journal of molecular sciencesAugust 18, 2022Brunello G, Zanotti F, Scortecci G, Sapoznikov L, Sivolella S, Zavan B.

(20) Anti-ACVR1 antibodies exacerbate heterotopic ossification in fibrodysplasia ossificans progressiva (FOP) by activating FOP-mutant ACVR1The Journal of clinical investigationJune 15, 2022Aykul S, Huang L, Wang L, Das NM, Reisman S, Ray Y, Zhang Q, Rothman N, Nannuru KC, Kamat V, et al.dissociation buffer (MilliPore, S-004-B) and resuspended in flow cytometry staining buffer (R&D Systems, FC001). After 15 minutes of blocking (Thermo Fisher Scientific, 14-9161-73), cells were stained with anti-ACVR1

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Flow Cytometry Staining Buffer (1X) from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Flow Cytometry Staining Buffer (1X) from R&D Systems, a Bio-Techne Brand

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