Fig 1: Transcription of XRN2 and IFIT3 mRNA is induced during RSV infection. HEp2 cells were infected with RSV or left uninfected (-) for indicated durations. Total RNA extracts were reverse transcribed and analyzed by qRT-PCR. Specific RNA expression levels were measured for IFIT3 and XRN2 and normalized to β-actin mRNA expression levels. Numbers above horizontal bars indicate n-fold change of the RNA expression level detected in infected cells relative to mock-infected cells.
Fig 2: XRN2 and IFIT3 expression during RSV infection. HEp2 cells were infected with RSV (+) or left uninfected (-) and harvested at indicated time points post infection. Cells were lysed and proteins analyzed by SDS-PAGE and subsequent immunoblotting using polyclonal antibody raised against IFIT3 (A) or either XRN2a or XRN2a/b (B). Western blot analysis of indicated offgel fractions of a whole cell lysate of RSV infected cells 24 hpi using a polyclonal antibody raised against XRN2a (C).
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