Fig 1: Characterization of the interaction between Integrator and AGO2.(A) Flag-AGO2 affinity purification, either without (left) or with (right) RNase treatment. (B) Flag-INTS11 affinity purification, either without (left) or with (right) RNase treatment. Inp., input; FT, flowthrough; E, elution. (C) Flag-Mock affinity purification. (D) Representative immunofluorescence of INTS11 (green; Abcam, #ab75276) and AGO2 (red; Abcam, #ab57113) and merged images including DAPI (4',6-diamidino-2-phenylindole; blue) in induced shControl HeLa cells. Scale bar, 50 µm. (E) Quantified Pearson colocalization values of AGO2-INTS11 compared to AGO2-DAPI and INTS11-DAPI using ImageJ 1.53o plug-in Coloc 2. (F) Representative immunofluorescence of INTS11 (green; Abcam, #ab75276) and pan-AGO (red; Abcam, #MABE56) and merged images including DAPI (blue) in induced shControl HeLa cells. Scale bar, 50 µm. (G) Quantified colocalization of pan-AGO2–INTS11 compared to pan-AGO2–DAPI and INTS11-DAPI.