EEA1 (C45B10) Rabbit mAb 3288S from Cell Signaling Technology

Product Specs
ItemEEA1 (C45B10) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number3288S
Quantity100 µl
ApplicationsW, IP, IF-F, IF-IC
ReactivityH, M, R
TargetEEA1
HostRabbit
NCBI Gene AliasesMST105, MSTP105, ZFYVE2
ClonalityMonoclonal
NCBI Full Gene Nameearly endosome antigen 1
Research AreaProtein Folding and Trafficking
IsotypeIgG
CloneC45B10
Molecular Weight170

Questions and Answers

What applications has the EEA1 antibody been used for in scientific research?

The EEA1 (C45B10) Rabbit mAb has been shown to work in applications such as Western blotting, immunocytochemistry, immunofluorescence, and immunoprecipitation. It has been utilized in studies to investigate endosomal trafficking and the role of EEA1 in various cellular processes.

Can this antibody be used to study endosomal dynamics in different species?

Yes, the EEA1 antibody is reactive with mouse, human, and rat samples, making it suitable for studies involving these species. This broad reactivity allows researchers to explore endosomal dynamics across different biological contexts.

Are there any specific studies that highlight the use of the EEA1 antibody in immunofluorescence?

Yes, the EEA1 antibody has been employed in immunofluorescence studies to stain early endosomes in various cell types. For instance, it was used in a study to visualize early endosomal compartments in cells treated with different stimuli, demonstrating its utility in examining endosomal trafficking pathways.

How has the EEA1 antibody contributed to understanding disease mechanisms?

The EEA1 antibody has been referenced in studies investigating the role of endosomal trafficking in diseases such as glioblastoma. For example, it was used to assess the impact of ionizing radiation on endosomal dynamics, which may enhance the delivery of therapeutic agents in cancer treatment, thereby providing insights into potential therapeutic strategies.

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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Cell swelling and upright mounting-based imaging for high-resolution visualization of intracellular trafficking across the BBB using conventional confocal microscopyDrug DeliveryDecember 31, 2026Da Hee Oh, Ji Hee Kang, O Hyun Lee, Young Tag Kocompartment,Dilution,Supplier,Cat. no. Early Endosome Antigen 1 (EEA1),Early Endosome (EE),1:1000,Cell Signaling Technology,3288S Rab5,Early Endosome (EE),1:1000,Cell Signaling Technology,3547S Rab11,Recycling Endosome (RE),1:1000,ABclonal,A3251 Vesicle-associated

(2) The use of genistein and ambroxol may be an effective approach in correcting cellular dysfunctions of mucopolysaccharidosis-plus syndromeMammalian GenomeDecember 1, 2026Zuzanna Cyske, Estera Rintz, Lidia Gaffke, Karolina Pierzynowska, Grzegorz Węgrzynwith 0.1% Tween-20). Membranes were incubated overnight at 4 °C with primary antibodies: anti-EEA1 (#3288S, Cell Signaling) and anti-LC3 (G-4, #sc-398822, Santa Cruz Biotechnology). After two washes in PBST,

(3) Control of lysosome function by the GTPase-activating protein TBC1D9B and its binding partner TMEM55BNat CommunMarch 14, 2026Valentin Duhay, Miaomiao Tian, Klaudia Kosieradzka, Michael Ebner, Wen-Ting Lo, Michael Krauss, Henner-Linus Sprengel, Matthias Voss, Mara Riechmann, Jeffrey N. Savas, et al.20281,RRID:AB_10853311 Cox IV (clone 3E11),Cell signaling,Cat# 4850,RRID:AB_2085424 EEA1 (clone C45B10,Cell signaling,Cat# 3288,RRID:AB_2096811 GFP (clone 7.1 + 13.1),Sigma Aldrich,Cat#11814460001,RRID:AB_390913 HA (clone 3F10),Sigma

(4) Amphotericin B promotes respiratory viral entry by enhancing late endosomal maturation and fusion via glucocerebrosidase-mediated ceramide remodelingNature CommunicationsMarch 9, 2026Di He, Wenting Zuo, Zhiguang Xiang, Jiankang Zhao, Wei Tong, Hongyan Li, Qing Fang, Xin Li, Yun Zhang, Ying Zheng, et al.were co-stained with anti-IAV NP (Cat# ab20343, 1:100; Abcam, USA) and either rabbit anti-EEA1 (Cat# 3288, 1:100; Cell Signaling Technology, USA) or rabbit anti-RAB7 (Cat# ab137029, 1:100; Abcam, USA). Colocalization

(5) Nur77 Regulates the Phosphorylation of Smad3, Thereby Influencing Skeletal Muscle Fibrosis Caused by ObesityFASEB JFebruary 15, 2026Na Li, Tingting Tian, Qihe Zhao, Difei Wangprimary antibodies: rabbit anti‐COL1A1 (Millipore, MAB348, 1:200 dilution), rabbit anti‐COL3A1 (CST, 3288, 1:100 dilution), mouse anti‐Rab7 (Santa Cruz, sc‐376 362, 1:100 dilution). Coverslips were then washed

(6) SLC33A1 exports oxidized glutathione to maintain endoplasmic reticulum redox homeostasisbioRxivFebruary 3, 2026Shanshan Liu, Mark Gad, Caifan Li, Kevin Cho, Yuyang Liu, Khando Wangdu, Viktor Belay, Alon Millet, Hiroyuki Kojima, Henry Sanford, et al.(65880S), CHOP (2895), ATF4(11815S), XBP-1s (40435S), SYVN1 (14773S), HA (2367S), HA (3724S), EEA1 (3288P) from Cell Signaling Technology; ATF6-N (A0202) from ABClonal; V5 (V8012–50UG), FLAG (F1804–200UG) from

(7) Cytoskeletal remodeling via CAMSAP3 downregulation drives resistance to osimertinib in NSCLC cellsCell Death & DiseaseDecember 11, 2025Fei Yang, Zhen Wang, Xiao Han, Jinjin Zhong, Zhanwu Hou, Shuying Bian, Jiangang Long, Huadong LiuSanta Cruz Biotechnology; Phospho-ERK1/2 (T202/Y204), TA1015, Abmart; ERK1/2, T40071, Abmart; EEA1, 3288T, Cell Signaling Technology; RAB11A, D4F5, Cell Signaling Technology; GAPDH, AC002, ABclonal; LAMP1,

(8) PPARγ mediated enhanced lipid biogenesis fuels Mycobacterium tuberculosis growth in a drug-tolerant hepatocyte environmentElifeDecember 8, 2025Binayak Sarkar, Jyotsna Jyotsna, Mohit Yadav, Priya Sharma, Raman Deep Sharma, Shweta Singh, Aakash Chandramouli, Kritee Mehdiratta, Ashwani Kumar, Siddhesh S Kamat, et al.Ab75852,RRID:AB_152367",Abcam,IF/ICC (1:150) 4.,LAMP1,"Cat#: 9091T,RRID:AB_2687579",CST,IF/ICC (1:200) 5.,EEA1,"Cat#: 3288T,RRID:AB_2096811",CST,IF/ICC (1:200) 6.,ASPGR1,"Cat#:PA5-32030,RRID:AB_2549503",Invitrogen,IF/ICC (1:500) 7.,PPAR

(9) Efficient cellular transformation via protein delivery through the protrusion-derived extracellular vesiclesNat CommunDecember 8, 2025Toshifumi Fujioka, Tamako Nishimura, Hiroki Kawana, Koichiro M. Hirosawa, Renta Yamakawa, Hani Sapili, Kayoko Oono-Yakura, Ryoya Nakagawa, Takanari Inoue, Osamu Nureki, et al.for WB), mouse anti-Annexin I (Santa Cruz, sc-12740, 1:1000 for WB), rabbit anti-EEA1 (Cell signaling, #3288, 1:100 for IF), mouse anti-Lamp1 (Santa Cruz, #sc-20011,1:50 for IF), mouse anti-β-actin (Santa Cruz,

(10) Mitochondrial components secretion in extracellular vesicles promotes alveolar epithelial mitochondrial quality controlNature CommunicationsDecember 5, 2025Bowen Liu, Yue Han, Yuan Jin, Meng Ye, Zeliang Yang, Jingyi Yang, Minglu Zhu, Xuyang Zhao, Yan Jin, Yuxin YinPrimary antibodies against the proteins are as followed: EEA1 (Cell Signaling Technology, Cat. No. 3288), CD63 (Santa Cruz, Cat. No. sc-5275) and LAMP1 (Cell Signaling Technology, Cat. No. 9091). Subsequently,

(11) A distinct PP2A subunit regulates local protein phosphorylation at the axon initial segmentNat CommunDecember 3, 2025Andrew P. Anderson, Sanghyun Kim, Allison J. Melton, Xiaoyun Ding, Wei Zhang, Alexander B. Saltzman, Anna Malovannaya, Matthew N. Rasband, Yudong Gao#29312-1-AP, 1:500), mouse anti-LAMP1 (Santa Cruz #sc-20011, 1:100), rabbit anti-EEA1 (Cell signaling #3288T, 1:500). For stimulated emission depletion (STED) super-resolution imaging, samples were blocked with

(12) Class A and B GPCRs trigger rapid Gαs translocation to late and slow recycling endosomesCommunications BiologyNovember 28, 2025Andréanne Laniel, Brian Holleran, Émy Labonté, Sarah-Janne Grondin, Pierre-Luc Boudreault, Christine LavoieUSA). Salmon sperm DNA (SSD) was from Invitrogen. Anti-Myc mouse (#2276) and anti-EEA1 rabbit 1:1000 (#3288) were purchased from Cell Signaling (Denver, Massachusetts, USA). HRP anti-rabbit IgG was purchased

(13) Annexin A7 enhances TIA1 axonal trafficking to counteract pathological aggregation in neuronsThe EMBO JournalNovember 3, 2025Yu Feng, Tongshu Luan, Zhenda Zhang, Wei Wang, Yuanyuan Chu, Sijia Wan, Xiaorong Pan, Jie Li, Yifan Liu, Yaqian Xu, et al.,Cell Signaling Technology,Cat#9091; RRID: AB_2687579 EEA1 (C45B10) Rabbit mAb,Cell Signaling Technology,Cat#3288; RRID: AB_2096811 DCP1A Polyclonal antibody,Proteintech,Cat#22373-1-AP; RRID: AB_2879092 TDP-43 (D9R3L)

(14) JIP4 and RILPL1 utilize opposing motor force to dynamically regulate lysosomal tubulationJournal of Cell BiologyNovember 3, 2025Luis Bonet-Ponce, Tsion Tegicho, Nuria Fernandez-Martinez, Irene A. Rozenberg, Mia Ashriem, Alexandra Beilina, Jillian H. Kluss, Yan Li, Mark R. Cookson1:500 for ICC), rat anti-myc (9E1; 1:20,000; ChromoTek for WB and 1:500 for ICC), rabbit anti-EEA1 (3288; 1:100; Cell Signaling Technology for ICC), rabbit anti-LC3B (2775; 1:1,000; Cell Signaling Technology

(15) The Chromosome 19 miRNA cluster guards trophoblasts against overacting innate immunityCommun BiolOctober 30, 2025Jean-Francois Mouillet, Yingshi Ouyang, Elena Sadovsky, Vinoth K. Kothandan, Heather L. Sorenson, Logan J. Badeau, Saumendra N. Sarkar, Tianjiao Chu, Alexander Sorkin, Yoel Sadovskywith unlabeled Poly(I:C) (InvivoGen) with anti-EEA1 (1:50, rabbit monoclonal, Cell Signaling, Cat# 3288) or anti-LAMP1 (1:50, rabbit monoclonal, Cell Signaling, Cat#9091) in 2% bovine serum albumin (BSA)

(16) Bergeyella cardium variant induces a unique cytoplasmic vacuolization cell death floatptosis in macrophageCell DiscovOctober 21, 2025Rudi Mao, Hongwei Pan, Luyu Yang, Zhenyu Fan, Yanfeng Li, Xinran Yu, Zhen Li, Ying Chen, Yang Yu, Wei Wang, et al.(CST, 9367); anti-SLC9A9 (Proteintech, 66577-1-Ig), anti-GST (Proteintech, 10000-0-AP), anti-EEA1 (CST,3288), anti-RAB5 (CST, 3574) or anti-cleaved caspase-3 (CST, 9661) — all at 1:300 to 1:500 dilution, overnight

(17) Endolysosomal dysfunction impairs proteostasis and induces neurodegeneration in vivoiScienceOctober 17, 2025Wei Shao, Ellen A. Albagli, Karen Jansen-West, Lillian M. Daughrity, Jimei Tong, Anxhela Hysi, Peizhou Jiang, Tiffany W. Todd, Emma Lee, Desiree Zanetti Alepuz, et al.IgG (H+L),Jackson ImmunoResearch,Cat# 611-035-215; RRID: AB_2721879 anti-EEA1,Cell Signaling,Cat# 3288S; RRID: AB_2096811 anti-CatD,ProteinTech,Cat# 21327-1-AP; RRID: AB_10733646 anti-Ubiquitin,Invitrogen,Cat#

(18) ALIX mediates reversible gasdermin-D pore formation via the endosomal pathway to limit pyroptosis by active membrane repairCell Death DisOctober 6, 2025Sylvia Otchere, Prafulla Shrestha, Himesh N. Parmar, Jadyn F. Perry, Brittany L. Hofmeister, Michelle Steyn, Radhey S. Kaushik, Adam D. Hoppe, Natalie W. Thiex, Ryan L. Hanson, et al.coverslips. The following dyes and primary antibodies were used: Anti-EEA1 (Cell Signaling Technology, 3288 T), Anti-CD81 (ABCAM, AB59477), Anti-N-terminal GSDMD (ABCAM, AB255983). Images were acquired using

(19) A Four Amino Acid Intracellular Motif of VISTA Blocks Growth Receptor Signaling in Cancer Cells to Induce Tumor SuppressionCancer ResearchSeptember 15, 2025Yan Zhao, Tina Andoh, Fatima Charles, Priyanka Reddy, Kristina Paul, Harsh Goar, Ishrat Durdana, Caiden J. Golder, Ashley N. Hardy, Marisa M. Juntilla, et al.α-caveolin (Cell Signaling Technology, #3267, RRID: AB_2275453), α-EEA1 (Cell Signaling Technology, #3288, RRID: AB_2096811), α-LAMP1 (Cell Signaling Technology, #9091, RRID: AB_2687579), α-CD4 (Cell Signaling Technology

(20) Fatty acid 2-hydroxylase facilitates rotavirus uncoating and endosomal escapeProceedings of the National Academy of SciencesSeptember 9, 2025Enkai Li, Ruochen Zang, Takahiro Kawagishi, Wei Zhang, Kruthika Iyer, Gaopeng Hou, Qiru Zeng, Rita M. Meganck, Susan R. Ross, Xin Wang, et al.permeabilized with 0.1% Triton X-100 and stained with the following primary antibodies: EEA1 (CST, #3288, 1:200), Rab7 (CST, #9367, 1:200), LAMP1 (CST, #9091, 1:200), and mAb159 (in-house, 1:200) (56). Stained

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5/11/2020Very Good EEA1 Antibody from CST Mark EUsed to detect endosomes with excellent signal.

EEA1 (C45B10) Rabbit mAb

EEA1 (C45B10) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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EEA1 (C45B10) Rabbit mAb from Cell Signaling Technology

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