PTEN (D4.3) XP ® Rabbit mAb 9188S from Cell Signaling Technology

Product Specs
ItemPTEN (D4.3) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $371.00 Inquire
Catalog Number9188S
Quantity100 µl
ApplicationsW, W-S, IP, IHC-Bond, IHC-P
ReactivityH, M, R, Mk, Dg
TargetPTEN
HostRabbit
NCBI Gene Aliases10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1
ClonalityMonoclonal
NCBI Full Gene Namephosphatase and tensin homolog
Research AreaPI3K / Akt Signaling
IsotypeIgG
CloneD4.3
Molecular Weight54

Questions and Answers

Can this antibody be used to study PTEN in mouse models, particularly in cancer research?

Yes, the PTEN (D4.3) XP® Rabbit mAb has been utilized in mouse models, as evidenced by its application in a study investigating the role of PTEN in prostate cancer using CRISPR/Cas9 technology. The antibody was employed in Western blotting to detect PTEN levels in mouse prostate tissues, highlighting its relevance in cancer research ().

Is this antibody effective for detecting PTEN in immunohistochemistry applications?

The PTEN (D4.3) XP® Rabbit mAb has been shown to work in immunohistochemistry applications, as indicated by its use in various studies that involved histochemical analysis of PTEN expression in tumor samples. This suggests that it is suitable for visualizing PTEN localization in tissue sections.

What species can this antibody detect, and is it suitable for studies involving human samples?

This antibody is reactive with several species, including human, mouse, rat, canine, and non-human primates. Its ability to detect endogenous levels of PTEN in human samples makes it a valuable tool for studies involving human cancer tissues and other biological samples.

Has this antibody been referenced in studies related to the PI3K/Akt signaling pathway?

Yes, the PTEN (D4.3) XP® Rabbit mAb has been cited in multiple studies focusing on the PI3K/Akt signaling pathway, which is crucial in cancer biology. For instance, it was used to assess PTEN levels in the context of PI3K/mTOR pathway inhibition in small cell lung cancer, demonstrating its importance in signaling pathway research ().

Cell Signaling Technology

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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) PTEN restrains SHH medulloblastoma growth through cell autonomous and nonautonomous mechanismseLifeApril 17, 2026Zhimin Lao, Salsabiel El Nagar, Yinwen Liang, Daniel N Stephen, Alexandra L JoynerLab,Cat# GFP-1010,IF (1:1000) Antibody,Rabbit monoclonal Pten (D4.3) XP,Cell Signalling Tech,Cat# 9188,IF (1:300) Antibody,Rabbit monoclonal Pten,Cell Signalling Tech,Cat# 9559,IF (1:200) Antibody,Rabbit

(2) Genome-wide profiling identifies the genetic dependencies of cell death following EGFR inhibitionJ Biol ChemApril 2, 2026Sydney A. Porto, Gavin A. Birdsall, Nicholas W. Harper, Megan E. Honeywell, Scott M. Leighow, Tiana E. Naylor, Kelly M. Ward, Mika K. Wesley, Justin R. Pritchard, Michael J. Lee3′ Immunoblotting Immunoblotting was performed using the LiCOR Odyssey CLx infrared scanner. PTEN (9188T), BIM (2933), Mcl-1 (5453S), and MYC (5605S) rabbit monoclonal antibodies were purchased from Cell Signaling Technologies

(3) Deficiency of PTEN Confers Hypersensitivity to Fatty Acid-Mediated ER Stress in Transformed HepatocytesInt J Mol SciMarch 19, 2026Olaya Yassin, Odai Darawshi, Fangfang Wang, Youwei Zhang, Ata Abbas, William C. Merrick, William Cheung, Antony Antoniou, Shakti P. Pattanayak, Boaz Tiroshlisted here: anti-PERK (CST #3192), anti-IRE1 (CST #3294), anti-pAKT (CST #13038), anti-PTEN (CST #9188), anti-Puromycin (Millipore Sigma, MABE341; St. Louis, MO, USA), anti-cMYC (abcam #ab32072), anti-P-S6-S240/244

(4) Suppression of PARP1 Enhances PTEN mRNA Therapy in Castration-Resistant Prostate Cancer by Glycolysis DisruptionMolecular Therapy OncologyMarch 19, 2026Bo Hu, Pengfei Wu, Lulu Zheng, Haiyin Yang, Yuchuan Fan, Jun Yang, Lu Tang, Sudong Kong, Chao Zhu, Minghui Yang, et al.(S3062), and Enhanced ATP Assay Kit (S0027) were purchased from Beyotime (Shanghai, China). PTEN mAb (9188), PARP mAb (9532), p-Akt (Ser 473) mAb (4060), PFKP mAb (8164), phospho-histone H2A.X (Ser139) mAb (9178),

(5) Ring finger protein 213 regulates B-cell receptor signaling, metabolism, and development in B lymphocytesSignal Transduction and Targeted TherapyFebruary 18, 2026Ziyin Zhang, Nanshu Xiang, Qian Liu, Yanfeng Li, Linhua Wang, Xinpu Yang, Ju Liu, Lichen Zhang, Liaoxun Lu, Yinming Liang, et al.(ABclonal, A20461), anti-GAPDH (Proteintech, 60004-1-Ig), anti-PIK3C3 (ABclonal, A12295), anti-PTEN (CST, 9188S), anti-pSHIP-1 (CST, 3941S), SHIP-1 (ABclonal, A3571), anti-NOTCH2 (ABclonal, A24877), anti-pHSL (CST,

(6) Targeting SH3GL1 for prognosis and immune response in breast cancerJournal of Pharmaceutical AnalysisJanuary 1, 2026Si Si, Hong Yu, Hao Zhang, Jianqiao Yin, Ziwei Li, Ning Wang, Xiaopeng Yurecombinant antibody (80570–1-RR, Proteintech, Chicago, IL, USA), P53 (sc-126, Santa, Dallas, TX, USA), PTEN (9188, Cell signaling, Danvers, MA, USA), RB1 (ab181616, Abcam, Cambridge, MA, USA), FDX1 (ab108257, Abcam,

(7) The microbial metabolite I3A inhibits ferroptosis and the effectiveness of redox-based cancer therapyJournal of Biological ChemistryJanuary 1, 2026Zixuan Guo, Lei Cui, Ruhui Yao, Yanxun Lin, Zining Wang, Huan Jin, Hui Guo, Chunyuan Xie, Lin Li, Peng Huang, et al.no.: 2920/4060), mTOR (CST; catalog no.: 2983), p53 (CST; catalog no.: 2524s), PTEN (CST; catalog no. 9188), HIF1A (CST; catalog no.: 36169), TFRC (Abcam; catalog no.: ab218544), ACTIN (Santa Cruz; catalog no.:

(8) PTEN-loss confers dependence on the guanylate synthesis enzyme IMPDH in T-cell acute lymphoblastic leukemiaBIORXIVDecember 24, 2025Rayees A. Padder, Thu Le Le, Emily Hyde, Maria E. D. Rubio, Eric Chiles, Namratha Sheshadri, Kelly Mulraney, Wei-Xing Zong, Xiaoyang Su, Daniel Herranz, et al.were performed using the following antibodies at 1:1000 dilution unless otherwise noted: PTEN (CST #9188, 1:2500), Cleaved Caspase- 3 (CST #9664), pH2AXS139 (CST #9718), CHK1 (CST #2360, 1:2000), pCHK1S345

(9) RNA and ChIP-sequencing analysis reveals SOX3 suppresses antiviral innate immunity through the AKT1-PTEN signaling axisVirologica SinicaDecember 5, 2025Tanzeel Yousaf, Jianwen Chen, Wajeeha Naz, Jiaqi Xu, Ying Liu, Junsong Huang, Siqi Yang, Jing Zhang, Iram Amin, Mingxiong Guo, et al.Phospho-AKT1 (Cell Signaling Technology, cat. no. 4060S), Phospho-PTEN (Cell Signaling Technology, cat. no. 9188S), Flag (Proteintech, cat. no. 20543-1-AP), GAPDH (Proteintech, cat. no. 10494-1-AP), and α-Tubulin (ABclonal,

(10) Developing a prognostic stratification model based on glutathione metabolism in thyroid cancer and validating RRM2’s tumor−promoting roleFrontiers in OncologyNovember 17, 2025Wei Ao, Teng-Hong Liu, De-Tao Yin, Wen-Xin Zhao(Cat.no. 4060)/Anti-Human PI3K Kinase p110α Antibody (Cat.no. 4249)/Anti-Human PTEN Antibody (Cat.no. 9188) were purchased from CST. Anti-Mouse PCNA (Cat.no. RMA-0145) and Anti-Mouse Ki-67 (Cat.no. RMA-0731)

(11) Schistosoma japonicum peptide SJMHE1 promotes peripheral nerve regeneration via macrophage migrasome-derived miR-26b-5p targeting the PTEN/AKT axisJournal of Translational MedicineNovember 11, 2025Qian Wang, Wei Zhang, Kai Zhao, Yue Liu, Shang Wang, Xingwen Chen, Dan Zhou, Juan Wang, Yu Feng, Tao Li, et al.16601-1-AP, Proteintech); β-actin (1:2000, 4970T, Cell Signaling Technology, Danvers, MA, USA); PTEN (1:1000, 9188T, Cell Signaling Technology), AKT (1:1000, 4691T, Cell Signaling Technology); phospho-AKT (1:1000, 4060T,

(12) PTEN restricts IL-21R signaling in GC B cells and suppresses their differentiation to plasma cellsThe Journal of ImmunologyNovember 1, 2025Alexander R Maldeney, Wenxia Jiang, Laura Conter, Daniel J Wikenheiser, William F Hawse, Anandkumar Patel, Weixun Peng, Mark J Shlomchik, Wei Luoclone 5E7), BLIMP1 (Al647, BioLegend Cat. no. 150004, clone 5E7), PTEN (Cell Signaling Technology, Cat: 9188, clone: D4.3), anti-Rabbit 2nd antibody (Cy3 conjugated, BioLegend Cat: 406402). For experiments using

(13) Sepsis-associated acute kidney injury in mice and humans elicits heterogeneous renal microvascular microRNA responsesSci RepOctober 2, 2025Matthijs Luxen, Tamar J. van der Aart, Rianne M. Jongman, Peter J. Zwiers, Jill Moser, Marianne Pultar, Susanna Skalicky, Andreas B. Diendorfer, Hjalmar R. Bouma, Jacqueline Koeze, et al.number Primary antibodies,Primary antibodies,Primary antibodies,Primary antibodies PTEN,0.007,Cell Signaling,9188 STAT3,0.044,Cell Signaling,9139 p-STAT3 (Y705),0.1,Cell Signaling,9145 GAPDH,0.04,SantaCruz,sc25778 Secondary

(14) PTEN loss and ERBB2/ERBB3-mediated AKT reactivation drive resistance to MET inhibition in MET-amplified hepatocellular carcinomaCell Oncol (Dordr)September 24, 2025Ruolan Qian, Yuchen Guo, Xiaolin Hu, Jing Ling, Haigang Geng, Qiaoqiao Ye, Linmeng Zhang, Shujie Zhan, Long Liao, Yang Ge, et al.(S1011), and Dacomitinib (S2727) were obtained from Selleck Chemicals. Primary antibodies targeting PTEN (9188), MET (8198), phospho-MET (3077), neurofibromin 1 (14623), phospho-AKT (4060), AKT (4685), ERBB2 (2165),

(15) Divergent FOXA1 mutations drive prostate tumorigenesis and therapy-resistant cellular plasticityScienceSeptember 4, 2025Sanjana Eyunni, Rahul Mannan, Yuping Zhang, Eleanor Young, Qiuyang Zhang, Jie Luo, Matthew Pang, Somnath Mahapatra, Jean Ching-Yi Tien, James M. George, et al.Signaling Technologies: 3638S); KLF5 (Proteintech: 21017–1-AP); PTEN (D4.3) (Cell Signaling Technology: 9188S); AKT (Cell Signaling Technology: 9272S); phospho-AKT (Ser473) (D9E) (Cell Signaling Technologies: 4060S);

(16) NF1 Loss Promotes EGFR Activation and Confers Sensitivity to EGFR Inhibition in NF1-Mutant MelanomaCancer ResearchSeptember 2, 2025Milad Ibrahim, Irineu Illa-Bochaca, George Jour, Eleazar Vega-Saenz de Miera, Joseph Fracasso, Kelly Ruggles, Iman Osman, Markus Schoberab140606), HSP90 (Cell Signaling Technology, 4877, RRID: AB_2233307), PTEN (Cell Signaling Technology, 9188, RRID: AB_2253290), and cPARP (Cell Signaling Technology, 5625, RRID:AB_10699459). Immunofluorescence

(17) PTEN deficiency in postnatally developing Purkinje cells disrupts metabolic signaling, leading to dendritic abnormalities and sex-specific behavioral deficitsSci RepJuly 8, 2025Lindsay J. Walsh, Izabella M. Espinal-San Miguel, Ana V. Rodriguez, Ursula M. Peña, Kiley E. Flynn, Will C. Remillard, Siena R. Brazier, Natalia I. Anderson, Aidan J. Clark, Tiffany A. De Varona, et al.and rat anti-CD107a (LAMP-1) (1:200, BioLegend, #121602), rabbit anti-PTEN (1:200, Cell signaling, #9188), guineapig anti-VGLUT2 (1:200, Synaptic Systems, #135 404), mouse anti-neurofilament (1:200, BioLegend,

(18) Early growth response 1 as a key regulator of PD-L1 expression and immune evasion in extranodal NK/T-cell lymphomaBlood Cancer JJune 13, 2025Ji Yun Lee, Kui-Jin Kim, Woochan Park, Jeongmin Seo, Minsu Kang, Eun Hee Jung, Sang-A Kim, Koung Jin Suh, Ji-Won Kim, Se Hyun Kim, et al.(ab78113), and PD-L1 (ab210931, ab213524) were purchased from Abcam (Cambridge, MA, USA). PTEN (cs#9188), p-AKT S473 (cs#4058), AKT (cs#9272), p-STAT3 T705 (cs#9145), β-actin (cs#4970), and vinculin (cs#9145)

(19) ER-α36 prevents high glucose-induced cellular senescence and apoptosis in renal tubular cellFront Endocrinol (Lausanne)June 9, 2025Dehai Yu, Ling Luo, Yu Liang, Huili Zhou, Yinghui Xiao, Xingna An, Yingzhao Wang, Zhonggao Xu, Weixia Sun, Wanning WangBcl-2 (1:1000, 3498S, Cell Signaling Technology), Bax (1:1000, 2772S, Cell Signaling Technology), PTEN (9188S, Cell Signaling Technology), PI3K (1:1000, 4249S, Cell Signaling Technology), phospho-PI3K (1:1000,

(20) Simvastatin overcomes the pPCK1‐pLDHA‐SPRINGlac axis‐mediated ferroptosis and chemo‐immunotherapy resistance in AKT‐hyperactivated intrahepatic cholangiocarcinomaCancer CommunicationsMay 29, 2025Jinghan Zhu, Yixiao Xiong, Yuxin Zhang, Huifang Liang, Kun Cheng, Yuanxiang Lu, Guangzhen Cai, Yang Wu, Yunhui Fan, Xiaoping Chen, et al.(MYMT2) (1:2,000, 13751‐1‐AP, Proteintech), anti‐phosphatase and tensin homolog (PTEN) (D4.3) (1:1,000, 9188, Cell Signaling Technology), anti‐SPRING (1:500, H00079794‐K, Abnova, Taipei, Taiwan), anti‐SREBP cleavage‐activating

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(1)

10/6/2020PTEN Antibody for Western Blot Sachin SamuchiwalI used this antibody to determine the levels of PTEN in 293T cells post transfection with siRNA against Akt.
(1)

2/25/2013PTEN XP Rabbit Monoclonal Antibody (D4.3) from Cell Signaling Technology Rachel Culpin, PhDPTEN is a tumor suppressor implicated in a wide variety of human cancers. It is a major negative regulator of the PI3K/Akt signaling pathway and also regulates p53 protein levels and activity. ... Read Review

PTEN (D4.3) XP ® Rabbit mAb

PTEN (D4.3) XP ® Rabbit mAb from Cell Signaling Technology

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PTEN (D4.3) XP ® Rabbit mAb from Cell Signaling Technology

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