Fig 2: FAM83A promoted LUAD cell migration ability. (a, b) The wound-healing assay showed significantly larger healing area in A549-shFAM83A and H1795-shFAM83A cells. (c, d) The transwell cell migration assay showed that in A549-shFAM83A and H1795-shFAM83A, more cells passed through the basement membrane. **P < 0.01, ***P < 0.001.

Fig 3: UALCAN analysis of TCGA showed FAM83A is overexpressed in LUAD. (a) UALCAN analysis showed FAM83A is overexpressed in lung adenocarcinoma (LUAD) and lung squamous carcinoma (LUSC), especially the former. (b, c) Cluster analysis showed a significant over expression of FAM83A in LUAD (tumor) than in normal lung tissue (normal). (d) Kaplan-Meier analysis showed that patients with high FAM83A expression had shorter overall survival, P < 0.0001.

Fig 4: FAM83A protein was overexpressed in LUAD and higher FAM83A expressions related to poor overall patient survival. (a) Representative hematein-eosin (HE) and immunohistochemistry (IHC) images of FAM83A expressions in adjacent normal tissues (NORMAL) and LUAD with low (LOW) and high (HIGH) FAM83A expressions. The short bar is equal to 50 microns; the long bar is equal to 100 microns. (b) The average optical density (AOD) of FAM83A in LUAD tissues and adjacent normal lung tissues. (c) AOD of FAM83A in LUAD tissues in stage I-II and stage III-IV. (d) Kaplan-Meier survival analysis showed a significant difference in 84 LUAD patients grouped by low and high FAM83A expression. *P < 0.05, **P < 0.01.

Fig 5: FAM83Apromoted proliferation of LUAD cells. (a, b) Western blotting analysis showed that FAM83A levels were higher in A549 and H1795 cells. (c) The reverse transcription-polymerase chain reaction (RT-PCR) assay demonstrated that A549 and H1795 cells transfected with FAM83A lentivirus (A549/H1795-shFAM83A), showed less FAM83A RNA expressions. (d) The cell proliferation assay showed no differences between A549-shFAM83A and A549-shRNA-NC cells at each time point. (e) The cell proliferation assay showed significant differences between H1795-shFAM83A and H1795-shRNA-NC cells. ***P < 0.001, ****P < 0.0001.