EPX Antibody (AHE-1) [Alexa Fluor® 488] NBP2-34551AF488 from Novus Biologicals, a Bio-Techne Brand

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Cell reports
July 26 2022

Fig 1: Eosinophils dominate the early granulocytic response in the airways of Mtb-infected rhesus macaques(A) Animal ID list of Mtb infections in rhesus macaques with representative FACS plots of EPX in BAL and quantification of eosinophils before and 1–2 weeks p.i. (n = 10, male [M] + female [F], matched Wilcoxon).(B) NHP granulocyte gating strategy and representative FACS plot with proportions of eosinophils (orange) and neutrophils (blue), alongside eosinophil/neutrophil ratio (E/N) in WB and BAL of uninfected rhesus macaques (n = 10, M + F, matched Wilcoxon).(C) NHP granulocyte gate frequency of eosinophils in BAL prior to and throughout Mtb infection (n = 3, ±SD).(D) E/N ratio in NHP BAL and WB throughout Mtb infection (n = 3; d7–d14 time points are highlighted in gray).
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Cell reports
July 26 2022

Fig 2: Eosinophils are recruited to lungs early after Mtb infection in mice and interact with Mtb-infected cells in the airways(A) Percentage and cell number of total lung eosinophils and neutrophils over time after aerosol infection (100–300 colony-forming units [CFU]) in WT mice (n = 6–25 per time point, M + F, 95% confidence interval [CI], SEM, 2 or 3 experiments per time point, *p = 0.04 and **p = 0.0032, unpaired t test).(B) Representative FACS plot of intravascular staining of eosinophils over time in mouse lung.(C) Percentage of lung parenchymal (CD45 i.v. negative) eosinophils and neutrophils over time after Mtb infection (n = 6–25 per time point, M + F, 95% CI, SEM, 2 or 3 experiments per time point, Mann-Whitney with indicated comparisons, *p = 0.0109, **p = 0.0032, and ****p < 0.0001).(D) Frequency of eosinophils in lung and whole blood after Mtb infection (n = 6–10 per time point, M + F, 95% CI, SEM, **p = 0.007, Mann-Whitney, 2 experiments).(E) Lung explant model of d13 Mtb-CFP (blue) infected mice expressing an EPX-eosinophil reporter (pink), representative still images (20 µm) from supplemental movie (2 experiments).(F) Representative FACS plots and quantification of H37Rv-mCherry-containing cells in lung on d14 (n = 18, M + F) of eosinophils (orange), neutrophils (blue), AMs (green) or gated on non-phagocytic, non-myeloid-lineage control cells (gray); dotted line is average signal of control cells (Wilcoxon-matched pairs test for eosinophil comparisons, ****p < 0.0001, 4 experiments).