Fig 1: SON depletion reduces but does not eliminate early centriole assembly. (A) SIM images of siRNA-treated, S phase-arrested cells induced for PLK4 expression and stained for centriole assembly factors. Scale bar = 1 μm. (B) Fluorescence intensity quantifications within a 1-µm radius circle of widefield microscopy images (Supplemental Figure S2A). N = GFP-Cetn: siControl, 851 centrosomes; siSON, 925 centrosomes; FLAG-PLK4: siControl, 111 centrosomes; siSON, 131 centrosomes; CEP152: siControl, 136 centrosomes; siSON, 143 centrosomes; CEP192: siControl, 124 centrosomes; siSON, 123 centrosomes; STIL: siControl, 136 centrosomes; siSON 138 centrosomes; SAS-6: siControl, 132 centrosomes; siSON, 148 centrosomes; CPAP: siControl, 120 centrosomes; siSON, 122 centrosomes; Cep135: siControl, 91 centrosomes; siSON, 119 centrosomes. Data were compiled from two biological replicates. Bar and error bars: mean and SD.