AceCS1 (D19C6) Rabbit mAb 3658S from Cell Signaling Technology

Product Specs
ItemAceCS1 (D19C6) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number3658S
Quantity100 µl
ApplicationsW, W-S, IP
ReactivityH, M, R, Mk
Targetacetyl-CoA synthetase
HostRabbit
NCBI Gene AliasesACAS2, ACECS, ACS, ACSA, AceCS1, dJ1161H23.1
ClonalityMonoclonal
NCBI Full Gene Nameacyl-CoA synthetase short chain family member 2
Research AreaMetabolism
IsotypeIgG
CloneD19C6
Molecular Weight78

Questions and Answers

Can this antibody be used to study the role of acetyl-CoA synthetase 2 in cancer metabolism?

Yes, the AceCS1 (D19C6) Rabbit mAb has been shown to detect endogenous levels of acetyl-CoA synthetase 2 (ACSS2), which plays a crucial role in converting acetate to acetyl-CoA, a key metabolite in lipid synthesis and energy production. Studies have indicated that ACSS2 is elevated in certain cancers and is important for cell survival under metabolic stress, making it relevant for cancer metabolism research.

What experimental techniques can I use this antibody with?

The AceCS1 (D19C6) Rabbit mAb is suitable for use in Western blotting (WB) and immunoprecipitation (IP). These techniques have been utilized in various studies to analyze ACSS2 expression and its functional implications in different cellular contexts, including cancer cell lines.

Is this antibody effective in different species, and which ones?

The AceCS1 (D19C6) Rabbit mAb has been shown to react with multiple species, including humans, mice, rats, and non-human primates. This broad reactivity allows for comparative studies across different model organisms in metabolic research.

Are there any specific findings in the literature regarding the use of this antibody in cancer research?

Yes, the antibody has been cited in studies that explore the regulation of ACSS2 in response to treatments like 4-hydroxytamoxifen in breast cancer cells. Research indicates that ACSS2 is crucial for the survival of estrogen receptor-positive breast cancer cells under estrogen deprivation, highlighting its potential as a therapeutic target in cancer treatment strategies.

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Website: Technical Support

(1) Trifluoperazine upregulates ACSS2-related autophagy and promotes cerebral cognitive function in rats after sleep deprivationiScienceMay 15, 2026Caijun He, Biao Wang, Xuanyu Chen, Jiacheng Xu, Yaxin Yang, Mei YuanRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Calmodulin,sigma,Cat#05-173 ACSS2,CST,Cat#3658S Beta Tubulin,Proteintech,Cat#10068-1-AP Goat anti-mouse IgG (H + L),Proteintech,Cat#SA00001-1 Goat

(2) ACSS2-mediated lysine crotonylation attenuates senescence and enhances the therapeutic efficacy of adipose-derived stem cells in inflammatory bowel diseaseCell RegenMarch 1, 2026Ming Yuan, Senmao Li, Shaopeng Chen, Minghui Zhu, Runfeng Yu, Junfeng Huang, Guanzhan Liang, Chi Zhang, Xiaowen He, Ping Lan, et al.#23,200, 1:1000), β-actin (Servicebio, #GB15003, 1:3000), Pan-Kcr (PTMBIO, #PTM-501, 1:1000), ACSS2 (CST, #3658, 1:1000), H3K9cr (PTMBIO, #PTM-539, 1:1000), H3 (Proteintech, #17,168–1-AP, 1:1000), p65 (CST, #8242,

(3) METTL3 abrogation promotes glioma progression through regulating the ISG15-FASN axis-mediated lipid metabolism in macrophagesNature CommunicationsJanuary 8, 2026Huilong Yin, Xiaoyi Yu, Chenglong Hu, Yuhang Yang, Mengke Wang, Yating Xing, Baile Zuo, Chenxing Zhang, Weilong Yang, Yitong Li, et al.Cell Signaling Technology), rabbit anti-PPARγ (1:1000, 2435S, Cell Signaling Technology), rabbit anti-AceCS1 (1:1000, 3658S, Cell Signaling Technology), Mouse anti-ISG15 (1:1000, sc-166755, Santa Cruz Biotechnology), mouse anti-FLAG

(4) Molecular Insights into Central Core Disease: Proteomic Signatures and Potential Therapeutic Biomarkers in RYR1 I4895T MiceInternational Journal of Molecular SciencesNovember 26, 2025Lorenza Vantaggiato, Enxhi Shaba, Federica Fiore, Daniela Rossi, Vincenzo Sorrentino, Luca Bini, Claudia LandiWB 1:1000); Anti-LDHB (Cell Signaling, Cat. #56298, WB 1:1000); Anti-AceCS1 (Cell Signaling, Cat. #3658, WB 1:1000); Anti-PGC1 alpha Antibody (Antibodies, Cat. #A8783, WB 1:1000); Anti-NDUFS1 (Cell Signaling,

(5) Identification of a common ketohexokinase-dependent link driving alcohol intake and alcohol-associated liver disease in miceNature MetabolismNovember 10, 2025Ana Andres-Hernando, David J. Orlicky, Gabriela E. Garcia, Esteban C. Loetz, Richard Montoya, Vijay Kumar, Devin P. Effinger, Masanari Kuwabara, So Young Bae, Laura Lorenzo-Rebenaque, et al.12035; RRID: RRID:AB_2797805), ALDH2 (Cell Signaling 18818; RRID:AB_2798804), AceCS1 (Cell Signaling 3658; RRID: RRID:AB_2222710), FAS (Cell Signaling 4233; RRID:AB_2100359), ACC (Cell Signaling 3676; RRID:AB_2219397),

(6) ACSS2 mediates an epigenetic pathway to regulate β-cell adaptation during gestation in miceNat CommunMay 20, 2025Yu Zhang, Shuang He, Xi Wang, Xin Wang, Mao-Yang He, Xin-Xin Yu, Cheng-Ran Xu28518012, dilution: 1:400), anti-STAT3 (CST, Cat# 12640, Lot# 4, dilution: 1:500), anti-ACSS2 (CST, Cat# 3658S, Lot# 2, dilution: 1:500), anti-glucagon (Takara, Cat# M182, Lot# AL32797A, dilution: 1:2,000), anti-Serotonin

(7) KRAS G12V mutation-selective requirement for ACSS2 in colorectal adenoma formationCell ReportsApril 22, 2025Konstantin Budagyan, Alexa C. Cannon, Adam Chatoff, Dorothy Benton, Alison M. Kurimchak, Daniela Araiza-Olivera, Anastasiia Gerasimova, Nathaniel W. Snyder, James S. Duncan, Cristina Uribe-Alvarez, et al.,Cell Signaling Technology,Cat# 67648; RRID:AB_2910195; 1:1000 Rabbit anti AceCS1,Cell Signaling Technology,Cat# 3658; RRID:AB_2222710; 1:1000 Mouse anti ACLY,Cell Signaling Technology,Cat# 13390; RRID:AB_2798203; 1:1000 Rabbit

(8) ATF6β is not essential for the development of physiological cardiac hypertrophyPLoS OneApril 7, 2025Emery Davis, Mohammad-Reza Shokri, Mary B. Rowland, Thomas York, Caroline T. Cohen, Anna Grace Johnson, Patrick E. Moore, Saige Schweitzer, Jon Sin, Chuong Bui, et al.Signaling Technology); P-Akt (S473) (D9E) XP(R), 1:1,000 (Cell Signaling Technology); AceCS1 (D19C6) #3658, 1:1,000 (Cell Signaling Technology); Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) #11818, 1:1000

(9) HSD17B4 deficiency causes dysregulation of primary cilia and is alleviated by acetyl-CoANat CommunMarch 18, 2025Ji-Eun Bae, Soyoung Jang, Joon Bum Kim, Na Yeon Park, Doo Sin Jo, Hyejin Hyung, Pansoo Kim, Min-Seon Kim, Hong-Yeoul Ryu, Hyun-Shik Lee, et al.T7451, Sigma-Aldrich), and anti-ACTA1 (1:10000, MAB1501, Millipore, Temecula, CA), anti-ACSS2 (1:3000, #3658S, Cell signaling). For protein detection, the membranes were incubated with horseradish peroxidase (HRP)-conjugated

(10) Class I histone deacetylases catalyze lysine lactylationbioRxivMarch 1, 2025Takeshi Tsusaka, Mohd. Altaf Najar, Isha Sharma, Mariola M. Marcinkiewicz, Claudia Veronica Da Silva Da Silva Crispim, Nathaniel W. Snyder, George M. Burslem, Emily L. Goldberg5113), anti-HDAC3 (1:500, CST, 3949), anti-FLAG (1:3000, Sigma-aldrich, F3165), anti-ACSS2 (1:1000, CST, 3658), Goat anti-Rabbit IgG HRP (1:5000, Thermo Scientific, 31460), Goat anti-Mouse IgG HRP (1:5000, Thermo Scientific

(11) ACSS2 drives senescence-associated secretory phenotype by limiting purine biosynthesis through PAICS acetylationNature communicationsFebruary 28, 2025Yang L, You J, Yang X, Jiao R, Xu J, Zhang Y, Mi W, Zhu L, Ye Y, Ren R, et al.western blots: anti-ACSS2 (Boster, cat. no. BM4830; 2 μg per immunoprecipitation, Cell Signaling, cat. no. 3658; 1:1000 for western blots), anti-ACLY (Cell Signaling, cat. no. 4332; 1:1000 for western blots), anti-LC3

(12) Acetyl-CoA synthesis in the skin is a key determinant of systemic lipid homeostasisCell ReportsFebruary 25, 2025Phuong T.T. Nguyen, Mia Shiue, Nina Kuprasertkul, Pedro Costa-Pinheiro, Luke T. Izzo, Laura V. Pinheiro, Hayley A. Affronti, Gabriel Gugiu, Shivani Ghaisas, Joyce Y. Liu, et al.(immunofluorescent staining),Abcam,Cat#ab40793; RRID: AB_722533 Rabbit monoclonal anti-AceCS1 (ACSS2),Cell Signaling Technology,Cat#3658; RRID: AB_2222710 Rabbit polyclonal anti-acetylated histone H3,Millipore,Cat#06-599; RRID: AB_2115283 Rabbit

(13) A genetically encoded fluorescent biosensor for visualization of acetyl-CoA in live cellsCell Chemical BiologyFebruary 20, 2025Joseph J. Smith, Taylor R. Valentino, Austin H. Ablicki, Riddhidev Banerjee, Adam R. Colligan, Debra M. Eckert, Gabrielle A. Desjardins, Katharine L. Diehlanti-ACLY mAb,Cell Signaling Technologies,Cat#13390 Rabbit anti-ACSS2 mAb,Cell Signaling Technologies,Cat#3658 Rabbit anti-PDH mAb,Cell Signaling Technologies,Cat#3205 Rabbit anti-CrAT pAb,Proteintech,Cat#15170-1-AP Rabbit

(14) Bempedoic acid suppresses diet-induced hepatic steatosis independently of ATP-citrate lyaseCell MetabolismJanuary 7, 2025Joyce Y. Liu, Ramya S. Kuna, Laura V. Pinheiro, Phuong T. T. Nguyen, Jaclyn E. Welles, Jack M. Drummond, Nivitha Murali, Prateek V. Sharma, Julianna G. Supplee, Mia Shiue, et al.sample. Antibodies used in this study were: ACLY (Proteintech 15421-1-AP), ACSS2 (Cell Signaling Technology 3658S), ribosomal protein S6 (Cell Signaling Technology 2217S), tubulin (Cell Signaling Technology 2144S),

(15) ACSS1-dependent acetate utilization rewires mitochondrial metabolism to support AML and melanoma tumor growth and metastasisCell ReportsDecember 1, 2024Sabina I. Hlavaty, Kelsey N. Salcido, Katherine A. Pniewski, Dzmitry Mukha, Weili Ma, Toshitha Kannan, Joel Cassel, Yellamelli V.V. Srikanth, Qin Liu, Andrew Kossenkov, et al.directed against ACSS1 (ProteinTech, Cat.No. 17138–1-AP) and ACSS2 (Cell Signaling Technology, Cat.No. 3658) were used at a 1:1000 dilution. The primary antibody against beta-actin (ProteinTech, Cat.No. 66009)

(16) Novel Choline-Deficient and 0.1%-Methionine-Added High-Fat Diet Induces Burned-Out Metabolic-Dysfunction-Associated Steatohepatitis with Inflammation by Rapid Immune Cell Infiltration on Male MiceNutrientsNovember 29, 2024Takatoshi Sakaguchi, Yasuharu Nagahama, Nanako Hamada, Shailendra Kumar Singh, Hayato Mikami, Kazuhiko Maeda, Shizuo AkiraAbcam (Cambridge, UK); and PGC-1β (#22378-1-AP) from Proteintech (Rosemont, IL, USA); and AceCS1 (#3658), ACL (#4332), p-ACL (#4331), ACC (#3676), p-ACC (#11818), Fas (#3180), AMPK (#5831), p-AMPK (#2535),

(17) Cancer-associated fibroblast-derived acetate promotes pancreatic cancer development by altering polyamine metabolism via the ACSS2-SP1-SAT1 axisNature cell biologyApril 1, 2024Murthy D, Attri KS, Shukla SK, Thakur R, Chaika NV, He C, Wang D, Jha K, Dasgupta A, King RJ, et al.utilized the following primary antibodies: anti-ACLY (Cell Signaling, 4332), anti-ACeCS1 (Cell Signaling, 3658), anti-SAT1 (Cell Signaling, 61586), anti-SP1 (Cell Signaling, 9389), anti-acetyl-lysine (Cell Signaling,

(18) Transcriptional and metabolic effects of aspartate-glutamate carrier isoform 1 (AGC1) downregulation in mouse oligodendrocyte precursor cells (OPCs)Cellular & molecular biology lettersMarch 29, 2024Balboni N, Babini G, Poeta E, Protti M, Mercolini L, Magnifico MC, Barile SN, Massenzio F, Pignataro A, Giorgi FM, et al.antibodies: FASN (Proteintech; cat. 10624-2-AP, RRID:AB_2100801), ACSS1 (Cell Signaling Technology; cat. 3658, RRID:AB_2222710), SREBP1 (Novus; cat. NB600-582, RRID:AB_10001575), and GAPDH (Santa Cruz Biotechnology;

(19) KRAS mutation-selective requirement for ACSS2 in colorectal adenoma formationResearch SquareFebruary 23, 2024Konstantin Chernoff, Konstantin Budagyan, Alexa Chatoff, Nathaniel W. Chatoff, Nathaniel Kurimchak, James S. Kurimchak, Jonathan Chernoff(Cell Signaling #13038), pERK (Cell Signaling #4370), ERK (Cell Signaling #4695), AceCS1 (Cell Signaling #3658), pACLY (Cell Signaling #4331), ACLY (Cell Signaling #13390), SREBP (Santa Cruz #sc-13551), pACC (Cell Signaling

(20) Chronic hypoxia stabilizes 3βHSD1 via autophagy suppressionCell reportsJanuary 23, 2024Qin L, Berk M, Chung YM, Cui D, Zhu Z, Chakraborty AA, Sharifi N.anti-Acetyl-Histone H3 (Lys27),Cell Signaling,8173;RRID:AB_10949503 Rabbit monoclonal anti-ACSS2,Cell Signaling,3658;RRID:AB_2222710 Goat anti-rabbit IgG,Cell Signaling,7074;RRID:AB_2099233 Horse anti-mouse IgG,Cell Signaling

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AceCS1 (D19C6) Rabbit mAb

AceCS1 (D19C6) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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AceCS1 (D19C6) Rabbit mAb from Cell Signaling Technology

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