KAP-1 (C42G12) Rabbit mAb 4124S from Cell Signaling Technology

Product Specs
ItemKAP-1 (C42G12) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number4124S
Quantity100 µl
ApplicationsW, IHC-P, IF-IC, FC-FP
ReactivityH, M, R, Mk
TargetTIF1b
HostRabbit
NCBI Gene AliasesKAP1, PPP1R157, RNF96, TF1B, TIF1B, TIF1beta
ClonalityMonoclonal
NCBI Full Gene Nametripartite motif containing 28
Research AreaCell Cycle / Checkpoint Control
CloneC42G12
Molecular Weight100

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) TRIM28 is an essential regulator of three-dimensional chromatin state underpinning CD8+ T cell activationNat CommunJanuary 16, 2025Kun Wei, Ruifeng Li, Xiaohong Zhao, Bowen Xie, Tian Xie, Qinli Sun, Yongzhen Chen, Peng Wei, Wei Xu, Xinyi Guo, et al.analysis. The following antibodies were used for immunoblotting: anti-TRIM28 antibody (CST, catalog 4124), anti-CTCF (CST, catalog 3418), Anti-SMC1 (Bethyl Laboratories, A300-055A). Retrovirus packaging and

(2) Werner syndrome RECQ helicase participates in and directs maintenance of the protein complexes of constitutive heterochromatin in proliferating human cellsAgingOctober 17, 2024Lazarchuk P, Nguyen MM, Curca CM, Pavlova MN, Oshima J, Sidorova JM.(Novus), rabbit α-HP1α Cat. No. 2616 (CST); rabbit α-V5 Cat. No. 3202 (CST); rabbit α-KAP1 Cat. No. 4124 (CST); mouse α-HDAC2 Cat. No. 5113 (CST); rabbit α-HDAC2 Cat. No. 57156 (CST); rabbit α-LBR Cat. No.

(3) Wybutosine hypomodification of tRNAphe activates HERVK and impairs neuronal differentiationiScienceMay 17, 2024Sun C, Guo R, Ye X, Tang S, Chen M, Zhou P, Yang M, Liao C, Li H, Lin B, et al.antibodies used are as following: anti-TYW1(customized), anti-SMARCAD1(A06049-1,boster), anti-KAP1(4124s, CST),SETDB1(nbp-2-20321,Novus). RT-qPCR Total RNA was extracted using RNA purification kit (EZBioscience)

(4) ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustionNature communicationsJune 19, 2023Menolfi D, Lee BJ, Zhang H, Jiang W, Bowen NE, Wang Y, Zhao J, Holmes A, Gershik S, Rabadan R, et al.Lot.7), pKAP1 S824 (1:1000, A300-767A, Bethyl Laboratories), KAP1 (1:1000, TIF1β, C42G12 Cell Signaling, #4124), pCHK1 Ser245 (1:1000, 133D3 Cell Signaling, #2348), CHK1 (1:1000, 2G1D5 Cell Signaling, #2360), pRPA32

(5) Human DUX4 and mouse Dux interact with STAT1 and broadly inhibit interferon-stimulated gene inductioneLifeApril 24, 2023Spens AE, Sutliff NA, Bennett SR, Campbell AE, Tapscott SJ.application Antibody,Anti-TIF1 (TRIM28) [C42G12] (rabbit monoclonal),Cell Signaling Technology,Cat# 4124; RRID:AB_2209886,See 'Materials and methods' for dilution by application Antibody,Anti-rabbit secondary

(6) Exploration of nuclear body-enhanced sumoylation reveals that PML represses 2-cell features of embryonic stem cellsNature communicationsSeptember 29, 2022Tessier S, Ferhi O, Geoffroy MC, González-Prieto R, Canat A, Quentin S, Pla M, Niwa-Kawakita M, Bercier P, Rérolle D, et al.Cat#AB3875, Millipore); anti-SUMO-2 (1/1000; Cat#Ab81371, Millipore), anti-TRIM28/Kap1 (1/1000; Cat#4124; Cell signaling), anti-UBC9 (1/1000; Cat#610749, BD Biosciences), anti-Ubiquitin (1/1000; Cat#BML-PW8810,

(7) Postnatal expression of cell cycle promoter Fam64a causes heart dysfunction by inhibiting cardiomyocyte differentiation through repression of Klf15iScienceMay 20, 2022Hashimoto K, Kodama A, Ohira M, Kimoto M, Nakagawa R, Usui Y, Ujihara Y, Hanashima A, Mohri S.et al., 2017). Primary antibodies used were for Klf15 (NBP2-24635, Novus Biologicals, USA), Trim28 (#4124, Cell Signaling Technology, USA), GR (sc-393232, Santa Cruz Biotechnology, USA), Myh7 (A4963, ABclonal,

(8) A FBXO7/EYA2-SCF(FBXW7) axis promotes AXL-mediated maintenance of mesenchymal and immune evasion phenotypes of cancer cellsMolecular cellMarch 17, 2022Shen JZ, Qiu Z, Wu Q, Zhang G, Harris R, Sun D, Rantala J, Barshop WD, Zhao L, Lv D, et al.tag,Thermo Fisher Scientific,Cat# 71-5500; RRID:AB_2533988 Rabbit anti-human KAP1,Cell Signaling,Cat# 4124; RRID:AB_2209886 "Mouse anti-human PE-HLA-A, B, C",BioLegend,Cat# 311405; RRID:AB_314874 Mouse anti-mouse

(9) SNHG8 Promotes the Progression of Epstein–Barr Virus-Associated Gastric Cancer via Sponging miR-512-5p and Targeting TRIM28Frontiers in OncologyOctober 15, 2021Changyan Zou et al.incubated with 1:1,000 primary antibodies (purchased from CST, Danvers, MA, USA) of TRIM28 (Cat No. #4124), VIM (Cat No. 5741S), CCND1 (Cat No. 55506s), PCNA (Cat No. D3H8P), Bcl-2 (Cat No. 5071S), CDH1 (Cat

(10) Differences between intrinsic and acquired nucleoside analogue resistance in acute myeloid leukaemia cellsJournal of experimental & clinical cancer research : CROctober 12, 2021Rothenburger T, Thomas D, Schreiber Y, Wratil PR, Pflantz T, Knecht K, Digianantonio K, Temple J, Schneider C, Baldauf HM, et al.Chk2 (Cell Signaling, 2662S, 1:1000), pChk2 (Cell Signaling, 2661S, 1:1000), TIF-1β (Cell Signaling, 4124S, 1:1000), pTIF-1β (Cell Signaling, 4127S, 1:1000). Visualisation and quantification were performed using

(11) FATC Domain Deletion Compromises ATM Protein Stability, Blocks Lymphocyte Development, and Promotes LymphomagenesisJournal of immunology (Baltimore, Md. : 1950)March 15, 2021Milanovic M, Shao Z, Estes VM, Wang XS, Menolfi D, Lin X, Lee BJ, Xu J, Cupo OM, Wang D, et al.the study are: anti-ATM (Sigma, A1106), anti-pKAP1 S824 (Abcam, ab70369), anti-KAP1 (Cell Signaling, 4124), anti-CHK2 (BD Biosciences, 611570), anti-pH2AX Ser139 (Cell Signaling, 9718S), anti-H2AX (Millipore,

(12) Mapping of the contraction-induced phosphoproteome identifies TRIM28 as a significant regulator of skeletal muscle size and functionCell reportsMarch 2, 2021Steinert ND, Potts GK, Wilson GM, Klamen AM, Lin KH, Hermanson JB, McNally RM, Coon JJ, Hornberger TA.RRID:AB_2269803 "Anti-Rabbit Total Tif1 β (Kap-1, TRIM28) (C42G12)","Cell Signaling Technologies (Danvers, MA, USA)",Cat# 4124S; RRID: N/A Anti-Rabbit Total S6 Ribosomal Protein (5G10),"Cell Signaling Technologies (Danvers, MA,

(13) Cold-inducible RNA binding protein promotes breast cancer cell malignancy by regulating Cystatin C levelsRNAFebruary 1, 2021Alberto Indacochea, Santiago Guerrero, Macarena Ureña, Ferrán Araujo, Olga Coll, Matilde E. LLeonart, Fátima Gebaueranti-FLAG (Sigma F3615, 1:5000), anti-SLC25A10 (Sigma HPA023048, 1:500), anti-TRIM28 (Cell Signaling 4124S, 1:500). RNA-immunoprecipitation (RIP) Cells were washed with PBS and resuspended in lysis buffer (100 mM

(14) The Cancer-Associated ATM R3008H Mutation Reveals the Link between ATM Activation and Its ExchangeCancer researchJanuary 15, 2021Milanovic M, Houghton LM, Menolfi D, Lee JH, Yamamoto K, Li Y, Lee BJ, Xu J, Estes VM, Wang D, et al.anti-pATM S1981 (Cell Signaling, 4526), anti-pKAP1 S824 (Abcam, ab70369), anti-KAP1 (Cell Signaling, 4124), anti-CHK2 (BD Biosciences, 611570), anti-pH2AX Ser139 (Cell Signaling, 9718S), anti-H2AX (Millipore,

(15) Long Noncoding RNA AFAP1-AS1 Is a Critical Regulator of Nasopharyngeal Carcinoma TumorigenicityFrontiers in OncologyNovember 23, 2020Min Fang, Minjun Zhang, Yiqing Wang, Fangqiang Wei, Jianhui Wu, Xiaozhou Mou, Yigan Zhang, Xiaodong Liang, Jianming TangTechnology), Histone H3 (#4499, 1:1000, Cell Signaling Technology), TIF1α (ab38264, 1:1000, Abcam), TIF1β (#4124, 1:1000, Cell Signaling Technology), TIF1γ (#13387, 1:1000, Cell Signaling Technology), NCOA1 (#20301,

(16) RAD51-Mediated DNA Homologous Recombination Is Independent of PTEN Mutational StatusCancers (Basel)October 29, 2020Asha Sinha et al.(Cell Signaling Technology, cat# 4127, 1:1000), rabbit anti-KAP1 mAb (Cell Signaling Technology, cat# 4124, 1:1000), rabbit anti-p-ATMS1981 mAb (Cell Signaling Technology, cat# 13050, 1:1000), rabbit anti-ATM

(17) TRIM28 is a distinct prognostic biomarker that worsens the tumor immune microenvironment in lung adenocarcinomaAging (Albany NY)October 22, 2020Jie Liu, Xiao Han, Lijuan Chen, Dong Han, Xiaoqian Mu, Xiufeng Hu, Hongbo Wu, Huijuan Wu, Wenjing Liu, Yanqiu Zhaofor one hour. The primary antibodies used for immunodetection were rabbit monoclonal anti-TRIM28 (#4124, Cell Signaling Technology, USA), rabbit polyclonal anti-IRF5 (#20261, Cell Signaling Technology), rabbit

(18) Antibody RING-Mediated Destruction of Endogenous ProteinsMolecular cellJuly 2, 2020Ibrahim AFM, Shen L, Tatham MH, Dickerson D, Prescott AR, Abidi N, Xirodimas DP, Hay RT.2019",N/A Rabbit monoclonal anti NEDD8,Epitomics,1571-1 Rabbit monoclonal anti TRIM28,Cell Signaling Technology,4124 ,, "Chemicals, Peptides, and Recombinant Proteins","Chemicals, Peptides, and Recombinant Proteins","Chemicals,

(19) Dual-Color Plasmonic Nanosensor for Radiation DosimetryACS Applied Materials & InterfacesMay 20, 2020Yu Tao, Mingqiang Li, Xiangyu Liu, Kam W. Leong, Jean Gautier, Shan Zhaaccording to standard procedures using primary antibodies specific for KAP1 (TIF1β, C42G12 Cell Signaling, #4124), phosphor-KAP1 (A300-767A, Bethyl Laboratories), and α-tubulin (CP06, Calbiochem). HRP-conjugated antimouse

(20) The lncRNA PVT1 regulates nasopharyngeal carcinoma cell proliferation via activating the KAT2A acetyltransferase and stabilizing HIF-1αCell death and differentiationFebruary 1, 2020Wang Y, Chen W, Lian J, Zhang H, Yu B, Zhang M, Wei F, Wu J, Jiang J, Jia Y, et al.(#4499, 1:1000, Cell Signaling Technology), TIF1γ (#13387, 1:1000, Cell Signaling Technology), TIF1β (#4124, 1:1000, Cell Signaling Technology), TIF1α (ab38264, 1:1000, Abcam), NCOA1 (#20301, 1:1000, Cell Signaling Technology

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TIF1beta (C42G12) Rabbit mAb

KAP-1 (C42G12) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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KAP-1 (C42G12) Rabbit mAb from Cell Signaling Technology

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