IL-33 Antibody AF3625 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIL-33 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $539.00Supplier Page View Company Product Page
Catalog NumberAF3625
NCBI Gene AliasesC9orf26, NFEHEV, IL1F11, NF-HEV, DVS27
ApplicationsELISA Development,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Neutralization,ELISA Capture (Matched Antibody Pair),Immunofluorescence,Immunocytochemistry,Cell Culture,Dual RNAscope ISH-IHC Compatible,ELISA Capture
TargetIL-33
Conjugate/TagUnconjugated
IsotypeIgG
Quantity100 ug (also 25 ug)
HostGoat
ReactivityHuman,Mouse,Transgenic Mouse
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
NCBI Full Gene Nameinterleukin 33
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human IL-33, Ser112-Thr270, Accession # O95760
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human IL-33 in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant mouse (rm) IL-33 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this IL-33 antibody be used in immunohistochemistry for studying human tissues?

Yes, the IL-33 antibody has been utilized in immunohistochemistry to analyze human tissues, as demonstrated in studies investigating its role in conditions such as endometriosis and chronic periodontitis, where it was shown to be overexpressed in affected tissues (, ).

What species does this antibody react with, and how specific is it?

The IL-33 antibody is reactive with human (Homo sapiens) and mouse (Mus musculus) species. It has been shown to specifically detect human IL-33 in ELISAs and Western blots, with less than 0.05% cross-reactivity with recombinant mouse IL-33 in sandwich ELISAs.

Are there any notable applications of this antibody in recent research?

Yes, this antibody has been cited in various studies, including its role in promoting intestinal tumorigenesis in Apc Min/+ mice (), and its involvement in the immune response during COVID-19, where it was used to assess IL-33 levels in lung tissues ().

How has this antibody been validated in scientific literature?

The IL-33 antibody has been orthogonally validated and cited in at least 47 scientific articles, indicating its reliability and effectiveness in various applications such as Western blotting, ELISA, and immunofluorescence, as evidenced by its use in studies examining the cytokine's role in inflammatory and neoplastic processes (, ).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Dual and context-dependent role of the interleukin-33/soluble suppression of tumorigenicity 2 axis in obesity and adipose tissue inflammationMolecular MedicineMarch 12, 2026Marcos Casado, Javier Gómez-Ambrosi, Beatriz Ramírez, Sara Becerril, Amaia Rodríguez, Víctor Valentí, Rafael Moncada, Camilo Silva, Javier Escalada, Gema Frühbeck, et al.(1704159, Bio-Rad Laboratories) were incubated overnight at 4 °C with goat anti-human IL-33 antibody (AF3625, R&D Systems; 1:10,000). Detection was performed using horseradish peroxidase-conjugated anti-goat IgG

(2) Spatial Transcriptomics of TMJ Reveals a Remodeling Fibroblast‐Immune Microenvironment Driving Arthritis PainAdvanced ScienceMarch 1, 2026Ziying Lin, Supawadee Jariyasakulroj, Yang Shu, Jingyi Chen, Qing Chang, Pao‐Fen Ko, Yuyueyang Qiu, Feixiang Chen, David Ahn, Zhen Zhao, et al.system, AF‐291), Rabbit anti‐PDGFRα (Cell Signaling Technology, Cat #5241), Goat anti‐IL‐33 (R&D system, AF3625 and AF3626), Rabbit anti‐CD206 (Abcam, ab64693), Rabbit anti‐TUBB3 (Cell Signaling Technology, Cat #5666S),

(3) Fibroblast-derived alarmin promotes oral wound healing by activating regulatory T cells that relay pro-angiogenic and anti-inflammatory responsesCell ReportsJanuary 27, 2026Zhaoxu Chen, Zikeng Huang, William S. Kim, Rahul Debnath, Kawintip Prasongyuenyong, Brett P. DerGarabedian, Thao Do, MacKenzie A. Moore-Kosslow, Jonathan M. Korostoff, Chider Chen, et al.,Enzo Life Sciences,Cat. #: ALX-804-840-C100;RRID: AB_2051699 Goat polyclonal anti-human IL33,R&D Systems,Cat. #: AF3625;RRID: AB_1151900 Goat polyclonal anti-human PI16,R&D Systems,Cat. #: AF4980;RRID: AB_2163885 Mouse

(4) Expression Analysis of JCAD and IL-33 in Gingival Cancer Tumor AngiogenesisCancers (Basel)November 21, 2025Tatsuya Shirai, Yasumasa Kakei, Yumi Muraki, Tatsuya Nagano, Ratoe Suraya, Kaito Uryu, Daisuke Takeda, Manabu Shigeoka, Akira Kimoto, Takumi Hasegawa, et al.antibody (HPA017956, 1:500; Sigma-Aldrich, St. Louis, MO, USA), goat polyclonal anti-human IL-33 antibody (AF3625, 1:200; R&D Systems, Minneapolis, MN, USA), rabbit monoclonal anti-human CD105 (clone EP274, 1:500;

(5) Circulating innate lymphoid cells are dysregulated in patients with prostate cancerCell Mol Biol LettApril 18, 2025Daniela Claudia Maresca, Evelina La Civita, Benedetta Romano, Maria Rosaria Ambrosio, Fabio Somma, Tania Wyss, Bernardo Rocco, Valentina Rubino, Luigi Cari, Philippe Krebs, et al.for CD3 antigen. Sections were then stained with goat anti-human IL-33 (R&D Systems, polyclonal, # AF3625; dilution 1:400) and mouse anti-human CD3 (Diagnostics Biosystems, clone LN10, #NCL-L-CD3-565; dilution

(6) IL-33-activated ILC2s induce tertiary lymphoid structures in pancreatic cancerNatureJanuary 15, 2025Masataka Amisaki, Abderezak Zebboudj, Hiroshi Yano, Siqi Linsey Zhang, George Payne, Adrienne Kaya Chandra, Rebecca Yu, Pablo Guasp, Zachary M. Sethna, Akihiro Ohmoto, et al.used to block tissue sections for 30 min. The sections were incubated with anti-human IL-33 antibody (AF3625, R&D System) for 4 h, followed by 60 min with biotinylated rabbit anti-goat IgG (Vector labs) at 1:200

(7) Interleukin‐3 Modulates Macrophage Phagocytic Activity and Promotes Spinal Cord Injury RepairCNS Neuroscience & TherapeuticsDecember 19, 2024Jianjian Li, Meige Zheng, Fangru Ouyang, Jianan Ye, Jinxin Huang, Yuanzhe Zhao, Jingwen Wang, Fangli Shan, Ziyu Li, Shuishen Yu, et al.(1:10, 503902, Biolegend), rabbit anti‐IL‐3Rα (1:100, 141039, US Biological), goat anti‐CD31 (1:200, AF3625, R&D Systems), rat anti‐F4/80 (1:100, 14‐4801‐82, Invitrogen), goat anti‐PDGFRβ (1:100, AF1042‐SP, R&D Systems

(8) Oncogenic KRAS-Dependent Stromal Interleukin-33 Directs the Pancreatic Microenvironment to Promote Tumor GrowthCancer discoveryOctober 4, 2024Donahue KL, Watkoske HR, Kadiyala P, Du W, Brown K, Scales MK, Elhossiny AM, Espinoza CE, Lasse Opsahl EL, Griffith BD, et al.

(9) Activator protein transcription factors coordinate human IL-33 expression from noncanonical promoters in chronic airway diseaseJCI insightMarch 8, 2024Raphael HE, Hassan GF, Osorio OA, Cohen LS, Payne MD, Katz-Kiriakos E, Tata I, Hicks J, Byers DE, Zhang B, et al.

(10) Symbiotic microbiome Staphylococcus epidermidis restricts IL-33 production in allergic nasal epithelium via limiting the cellular necroptosisBMC microbiologyMay 26, 2023Jeon YJ, Gil CH, Won J, Jo A, Kim HJ.(Foster City, CA, USA). Western blot Human primary IL-33 antibody was purchased from R&D Systems (AF3625; Minneapolis, MN, USA). Human primary antibodies for RIPK3 (PA5-19956), phospho-MLKL (Ser358, PA5-105678),

(11) The IL-33:ST2 axis is unlikely to play a central fibrogenic role in idiopathic pulmonary fibrosisRespiratory researchMarch 23, 2023Stephenson KE, Porte J, Kelly A, Wallace WA, Huntington CE, Overed-Sayer CL, Cohen ES, Jenkins RG, John AE.previously described [29, 33]. Primary antibodies used for western blotting included goat anti-IL-33 (AF3625; R&D systems), goat anti-ST2 (AF523; R&D systems), mouse anti-α-Tubulin (TU-02; Santa Cruz Biotechnology,

(12) Imatinib inhibits pericyte-fibroblast transition and inflammation and promotes axon regeneration by blocking the PDGF-BB/PDGFRβ pathway in spinal cord injuryInflammation and regenerationSeptember 26, 2022Yao F, Luo Y, Liu YC, Chen YH, Li YT, Hu XY, You XY, Yu SS, Li ZY, Chen L, et al.0.3% Triton X-100 overnight at 4°C. The primary antibodies used were as follows: goat anti-CD31(1:100, AF3625, R&D Systems), rat anti-PDGFRβ (1:100, 14-1402-82, Invitrogen), goat anti-PDGFRβ (1:100, AF1042, R&D Systems

(13) Organoids capture tissue-specific innate lymphoid cell development in mice and humansCell reportsAugust 30, 2022Jowett GM, Read E, Roberts LB, Coman D, Vilà González M, Zabinski T, Niazi U, Reis R, Trieu TJ, Danovi D, et al.

(14) Fungal mycobiome drives IL-33 secretion and type 2 immunity in pancreatic cancerCancer cellFebruary 14, 2022Alam A, Levanduski E, Denz P, Villavicencio HS, Bhatta M, Alhorebi L, Zhang Y, Gomez EC, Morreale B, Senchanthisai S, et al.

(15) Nuclear IL-33 regulates cytokinesis and cell motility in normal human epidermal keratinocytesJournal of dermatological scienceFebruary 1, 2022Tsuda H, Tominaga SI, Ohtsuki M, Komine M.

(16) Eosinophils regulate intra-adipose axonal plasticityProceedings of the National Academy of Sciences of the United States of AmericaJanuary 18, 2022Meng X, Qian X, Ding X, Wang W, Yin X, Zhuang G, Zeng W.anti-mouse IL-33 (R&D Systems catalog no. AF3626, RRID: AB_884269), goat anti-human IL-33 (R&D Systems catalog no. AF3625, RRID: AB_1151900), mouse anti-human Siglec-8 (BioLegend catalog no. 347102, RRID: AB_2239311), rat

(17) Human Melanoma-Associated Mast Cells Display a Distinct Transcriptional Signature Characterized by an Upregulation of the Complement Component 3 That Correlates With Poor PrognosisFrontiers in immunologyJanuary 1, 2022Bahri R, Kiss O, Prise I, Garcia-Rodriguez KM, Atmoko H, Martínez-Gómez JM, Levesque MP, Dummer R, Smith MP, Wellbrock C, et al.blocking antibodies were used with their respective isotype controls: anti-human IL-33 (50 μg/ml) (AF3625), goat IgG control (AB-108-C), anti-human IL-1β (50 μg/ml) (clone: 8516, MAB201), and mouse IgG1 isotype

(18) IL-33 activates group 2 innate lymphoid cell expansion and modulates endometriosisJCI insightDecember 8, 2021Miller JE, Lingegowda H, Symons LK, Bougie O, Young SL, Lessey BA, Koti M, Tayade C.temperature. The following primary antibodies were used: Goat Anti-human IL-33 (1:40, R&D Systems, AF3625), Mouse Anti-human Vimentin (1:400, Cell Signaling Technology, clone D21H3), Mouse Anti-Pan cytokeratin

(19) The impact of airborne endotoxin exposure on rheumatoid arthritis-related joint damage, autoantigen expression, autoimmunity, and lung diseaseInternational immunopharmacologyNovember 1, 2021Mikuls TR, Gaurav R, Thiele GM, England BR, Wolfe MG, Shaw BP, Bailey KL, Wyatt TA, Nelson AJ, Duryee MJ, et al.(n=9) were stained with mouse anti-IL-33 (AF3626, 2.5 μg/100 μl; R&D Systems) or human anti-IL-33 (AF3625, 2.5 μg/100 μl) and anti-vimentin (Abcam, AB 92547, 1:200). Donkey anti-goat (AlexaFluorPlus555, A32816)

(20) IL-33 Depletion in COVID-19 LungsChestNovember 1, 2021Gaurav R, Anderson DR, Radio SJ, Bailey KL, England BR, Mikuls TR, Thiele GM, Strah HM, Romberger DJ, Wyatt TA, et al.transplant bio-bank through an honest broker. Sections were stained with human anti-IL-33 (R&D Systems, AF3625, Lot #YYZ0918051, 2.5 μg/100 μL), prosurfactant protein C (Millipore Sigma, AB3786, Lot #3466551, 1:200),

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(1)

12/12/2016Good Antibody To Detect IL33 By Immunostaining Sunita MeenaIn the present study, IL33 proteins were immunostained in the pancreatic sections of cholesterol induced diabetic and non-diabetic rats. Further, primary antibodies were identified by alexa fluor ... Read Review
(1)

6/24/2016Excellent Antibody to Detect IL-33 in Human Cells Raj KumarAim of the study was to test the role of specific growth factor on IL-33 expression in human smooth muscle cell. Proteins from each treatment were extracted and resolved in SDS PAGE. Resolved proteins... Read Review

IL-33 Antibody

IL-33 Antibody

IL-33 Antibody

IL-33 Antibody

IL-33 Antibody

IL-33 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (62)

Reviews

IL-33 Antibody from R&D Systems, a Bio-Techne Brand

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